Berglund:Kristy Method-U2AF65

Materials

10X Binding Buffer C (BBC)
250mM Tris pH 7.5
500mM NaCl
10mM DTT
10mM EDTA

10X Binding Buffer D (BBD)
500 mM Tris pH 7.5
50 mM MgCl2
1 M NaCl
0.1% Triton X-100
10mM DTT

10X native dye
200µL 5X TBE or TB
400µL 100% glycerol
250µL 2% bromophenol blue
150µL H2O

recombinant purified U2AF65
kinased RNA of your choice

Method