Berglund:Large Scale: 10ml: Difference between revisions
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*2mM spermidine | *2mM spermidine | ||
*radioactive nucleotide? | *radioactive nucleotide? | ||
*T7 RNA poly | |||
==Procedure== | ==Procedure== |
Latest revision as of 17:04, 24 September 2008
Overview
Protocol from Mary, as yet untested in our lab.
Materials
- 20µg/mL linearized plasmid DNA
- 40mM Tris pH 8.0
- 10mM DTT
- 10mM MgCl2
- 1mM each rNTP
- 2mM spermidine
- radioactive nucleotide?
- T7 RNA poly
Procedure
- Incubate at 37°C for 5 hours.
- PAGE purify.
Notes
UNTESTED. Consult with Amy before proceeding.