Berglund:Protocols

http://openwetware.org/wiki/Berglund_Lab

Gels Agarose SDS-PAGE Acrylamide Native Acrylamide Acrylamide-Agarose	Nucleic Acid PCR: Commonly Used Primers Plasmids PCR: Various Methods Transcription: Various Methods Kinasing Phenol/Ethanol Extraction RNA column purification making rNTP calculating RNA molecular weight	Structure Footprinting UV crosslinking SHAPE Crystallography Analytical computer programs
Protein Protein Buffers Protein Purification: Various Methods Binding Gels: Various Methods Protein Concentration Assay: Various Methods Western Blot Coomassie Blue Staining Silverstaining	Cloning and Bacteria Cells Available Making Competent Cells: Various Methods Chemical Transformation Electroporation Cloning: Various Methods	General Buffers Machines Available for Use Formerly Used Protocols Worm Fix
Antibody Work List of Antibodies Available Antibody Storage Antibody Purification Antibody Making/Ordering	Splicing In Vivo In Vivo Cells Available General Cell Culture Methods Solutions Methods of Analysis In Vitro Background Complex Formation Splicing	Nanoparticle Work Reagents Method A Method B Using the TEM Equipment available