Berglund:Protocols: Difference between revisions
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<big><big>Gels</big></big> | <big><big>Gels</big></big> | ||
---- | ---- | ||
[[Berglund:Agarose|<h3><font color=#000000>Agarose</font></h3>]] | horizontal gels | ||
[[Berglund:SDS-PAGE|<h3><font color=#000000>SDS-PAGE</font></h3>]] | :[[Berglund:Agarose|<h3><font color=#000000>Agarose</font></h3>]] | ||
[[Berglund:Acrylamide|<h3><font color=#000000>Acrylamide</font></h3>]] | :[[Berglund:Denaturing Agarose|<h3><font color=#000000>Denaturing Agarose</font></h3>]] | ||
[[Berglund:Native Acrylamide|<h3><font color=#000000>Native Acrylamide</font></h3>]] | vertical gels | ||
[[Berglund:Acrylamide-Agarose|<h3><font color=#000000>Acrylamide-Agarose</font></h3>]] | :[[Berglund:SDS-PAGE|<h3><font color=#000000>SDS-PAGE</font></h3>]] | ||
:[[Berglund:Acrylamide|<h3><font color=#000000>Denaturing Acrylamide</font></h3>]] | |||
:[[Berglund:Native Acrylamide|<h3><font color=#000000>Native Acrylamide</font></h3>]] | |||
:[[Berglund:Acrylamide-Agarose|<h3><font color=#000000>Acrylamide-Agarose</font></h3>]] | |||
:[[Berglund:Native gels for in vivo splicing|<h3><font color=#000000>Native gels for in vivo splicing</font></h3>]] | |||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Nucleic Acid</big></big> | |||
[[Berglund:PCR: Commonly Used Primers|<h3><font color=#000000>PCR: Commonly Used Primers</font></h3>]] | ---- | ||
[[Berglund:PCR: Various Methods|<h3><font color=#000000>PCR: Various Methods</font></h3>]] | DNA | ||
[[Berglund:Transcription: Various Methods|<h3><font color=#000000>Transcription: Various Methods</font></h3>]] | :[[Berglund:PCR: Commonly Used Primers|<h3><font color=#000000>PCR: Commonly Used Primers</font></h3>]] | ||
[[Berglund: | :[[Berglund:Plasmids|<h3><font color=#000000>Plasmids</font></h3>]] | ||
[[Berglund: | :[[Berglund:PCR: Various Methods|<h3><font color=#000000>PCR: Various Methods</font></h3>]] | ||
[[Berglund: | RNA | ||
[[Berglund: | :[[Berglund:Transcription: Various Methods|<h3><font color=#000000>Transcription: Various Methods</font></h3>]] | ||
:[[Berglund: calculating RNA molecular weight|<h3><font color=#000000>calculating RNA molecular weight</font></h3>]] | |||
:[[Berglund:RNA column purification|<h3><font color=#000000>RNA column purification</font></h3>]] | |||
:[[Berglund: making rNTP|<h3><font color=#000000>making rNTP</font></h3>]] | |||
Labelling and Cleanup | |||
:[[Berglund:Kinasing|<h3><font color=#000000>Kinasing</font></h3>]] | |||
:[[Berglund:Phenol/Ethanol Extraction|<h3><font color=#000000>Phenol/Ethanol Extraction</font></h3>]] | |||
Blots | |||
:[[Berglund: Northern Blot|<h3><font color=#000000>Northern Blot</font></h3>]] | |||
</div> | </div> | ||
|width="33%" style="border: 1px solid #ff3333; color: #000; background-color: #FFFFFF"| | |width="33%" style="border: 1px solid #ff3333; color: #000; background-color: #FFFFFF"| | ||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Structure</big></big> | |||
---- | |||
[[Berglund:Footprinting|<h3><font color=#000000>Footprinting</font></h3>]] | [[Berglund:Footprinting|<h3><font color=#000000>Footprinting</font></h3>]] | ||
[[Berglund:UV crosslinking|<h3><font color=#000000>UV crosslinking</font></h3>]] | [[Berglund:UV crosslinking|<h3><font color=#000000>UV crosslinking</font></h3>]] | ||
[[Berglund: | [[Berglund:SHAPE2|<h3><font color=#000000>SHAPE (finished protocol)</font></h3>]] | ||
[[Berglund:Analytical computer programs|<h3><font color=#000000>Analytical computer programs</font></h3>]] | [[Berglund:Analytical computer programs|<h3><font color=#000000>Analytical computer programs</font></h3>]] | ||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Protein</big></big> | |||
[[Berglund:Protein Buffers|<h3><font color=#000000>Protein Buffers</font></h3>]] | ---- | ||
[[Berglund:Protein Purification: Various Methods|<h3><font color=#000000>Protein Purification: Various Methods</font></h3>]] | preparing your proteins | ||
[[Berglund: | :[[Berglund:Protein Buffers|<h3><font color=#000000>Protein Buffers</font></h3>]] | ||
[[Berglund: | :[[Berglund:Protein Purification: Various Methods|<h3><font color=#000000>Protein Purification: Various Methods</font></h3>]] | ||
[[Berglund: | :[[Berglund:Protein Concentration Assay: Various Methods|<h3><font color=#000000>Protein Concentration Assay: Various Methods</font></h3>]] | ||
[[Berglund: | staining gels | ||
[[Berglund: | :[[Berglund:Coomassie Blue Staining|<h3><font color=#000000>Coomassie Blue Staining</font></h3>]] | ||
:[[Berglund:Silverstaining|<h3><font color=#000000>Silverstaining</font></h3>]] | |||
using your protein | |||
:[[Berglund:GST pulldown example|<h3><font color=#000000>GST pulldown example</font></h3>]] | |||
:[[Berglund:Binding Gels: Various Methods|<h3><font color=#000000>Binding Gels: Various Methods</font></h3>]] | |||
:[[Berglund:Western Blot|<h3><font color=#000000>Western Blot</font></h3>]] | |||
</div> | </div> | ||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Cloning and Bacteria</big></big> | |||
---- | |||
[[Berglund:Cells Available|<h3><font color=#000000>Cells Available</font></h3>]] | [[Berglund:Cells Available|<h3><font color=#000000>Cells Available</font></h3>]] | ||
[[Berglund:Making Competent Cells: Various Methods|<h3><font color=#000000>Making Competent Cells: Various Methods</font></h3>]] | [[Berglund:Making Competent Cells: Various Methods|<h3><font color=#000000>Making Competent Cells: Various Methods</font></h3>]] | ||
[[Berglund:Chemical Transformation|<h3><font color=#000000>Chemical Transformation</font></h3>]] | [[Berglund:Chemical Transformation|<h3><font color=#000000>Chemical Transformation</font></h3>]] | ||
[[Berglund:Cloning: Various Methods|<h3><font color=#000000>Cloning: Various Methods</font></h3>]] | [[Berglund:Cloning: Various Methods|<h3><font color=#000000>Cloning: Various Methods</font></h3>]] | ||
[[Berglund:Plasmids|<h3><font color=#000000>Plasmids</font></h3>]] | |||
[[Berglund:NEB sheets for enzyme cutting|<h3><font color=#000000>NEB sheets for enzyme cutting</font></h3>]] | |||
</div> | </div> | ||
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<div style="clear: right; text-align: left; float: left; padding: .1em 1.5em .9em"> | <div style="clear: right; text-align: left; float: left; padding: .1em 1.5em .9em"> | ||
<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<!--------------------------------General----------------------------------> | <!--------------------------------General Recipes----------------------------------> | ||
<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>General Recipes</big></big> | |||
---- | |||
[[Berglund:Buffers|<h3><font color=#000000>Buffers</font></h3>]] | [[Berglund:Buffers|<h3><font color=#000000>Buffers</font></h3>]] | ||
[[Berglund:Worm Fix|<h3><font color=#000000>Worm Fix</font></h3>]] | [[Berglund:Worm Fix|<h3><font color=#000000>Worm Fix</font></h3>]] | ||
[[Berglund:2xDenaturing Dye|<h3><font color=#000000>2xDenaturing Dye</font></h3>]] | |||
[[Berglund:Spec and Qubit calculators|<h3><font color=#000000>Spec and Qubit calculators</font></h3>]] | |||
[[Berglund:Calculating solutions|<h3><font color=#000000>Calculating solutions</font></h3>]] | |||
[[Berglund:Calculating protein concentrations|<h3><font color=#000000>Calculating protein concentrations</font></h3>]] | |||
</div> | </div> | ||
|- | |- | ||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Antibody Work</big></big> | |||
---- | |||
[[Berglund:List of Antibodies Available|<h3><font color=#000000>List of Antibodies Available</font></h3>]] | [[Berglund:List of Antibodies Available|<h3><font color=#000000>List of Antibodies Available</font></h3>]] | ||
[[Berglund:Antibody Storage|<h3><font color=#000000>Antibody Storage</font></h3>]] | [[Berglund:Antibody Storage|<h3><font color=#000000>Antibody Storage</font></h3>]] | ||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Splicing</big></big> | |||
---- | |||
'''In Vivo'''<br> | '''In Vivo'''<br> | ||
[[Berglund:In Vivo Cells Available|<h3><font color=#000000>In Vivo Cells Available</font></h3>]] | :[[Berglund:Cell culture guide|<h3><font color=##CA0000>Proper cell culture technique</font></h3>]] | ||
[[Berglund:General Cell Culture Methods|<h3><font color=#000000>General Cell Culture Methods</font></h3>]] | :[[Berglund:In Vivo Cells Available|<h3><font color=#000000>In Vivo Cells Available</font></h3>]] | ||
[[Berglund: | :[[Berglund:General Cell Culture Methods|<h3><font color=#000000>General Cell Culture Methods</font></h3>]] | ||
[[Berglund: | :[[Berglund:Harvesting RNA|<h3><font color=#000000>Harvesting RNA</font></h3>]] | ||
:[[Berglund:Post-Harvesting RNA|<h3><font color=#000000>Post-Harvesting RNA</font></h3>]] | |||
:[[Berglund:Freezing cells|<h3><font color=#000000>Freezing cells</font></h3>]] | |||
'''In Vitro'''<br> | '''In Vitro'''<br> | ||
[[Berglund:Background|<h3><font color=#000000>Background</font></h3>]] | :[[Berglund:Background|<h3><font color=#000000>Background</font></h3>]] | ||
[[Berglund:Complex Formation|<h3><font color=#000000>Complex Formation</font></h3>]] | :[[Berglund:Complex Formation|<h3><font color=#000000>Complex Formation</font></h3>]] | ||
[[Berglund:Splicing|<h3><font color=#000000>Splicing</font></h3>]] | :[[Berglund:Splicing|<h3><font color=#000000>Splicing</font></h3>]] | ||
</div> | </div> | ||
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<!-------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------> | ||
<big><big>Equipment</big></big> | |||
---- | |||
[[Berglund:Using the TEM|<h3><font color=#000000>Using the TEM</font></h3>]] | [[Berglund:Using the TEM|<h3><font color=#000000>Using the TEM</font></h3>]] | ||
[[Berglund:Equipment available|<h3><font color=#000000>Equipment available</font></h3>]] | [[Berglund:Equipment available|<h3><font color=#000000>Equipment available</font></h3>]] |
Revision as of 09:43, 7 March 2014
http://openwetware.org/wiki/Berglund_Lab
Gels horizontal gels vertical gels
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Nucleic Acid DNA RNA Labelling and Cleanup Blots |
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Protein preparing your proteins staining gels using your protein |
Cloning and Bacteria Cells AvailableMaking Competent Cells: Various MethodsChemical TransformationCloning: Various MethodsPlasmidsNEB sheets for enzyme cutting
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