Berglund:Protocols

http://openwetware.org/wiki/Berglund_Lab

Gels horizontal gels Agarose Denaturing Agarose vertical gels SDS-PAGE Denaturing Acrylamide Native Acrylamide Acrylamide-Agarose Native gels for in vivo splicing	Nucleic Acid DNA PCR: Commonly Used Primers Plasmids PCR: Various Methods RNA Transcription: Various Methods calculating RNA molecular weight RNA column purification making rNTP Labelling and Cleanup Kinasing Phenol/Ethanol Extraction Blots Northern Blot	Structure Footprinting UV crosslinking SHAPE (finished protocol) Analytical computer programs
Protein preparing your proteins Protein Buffers Protein Purification: Various Methods Protein Concentration Assay: Various Methods staining gels Coomassie Blue Staining Silverstaining using your protein GST pulldown example Binding Gels: Various Methods Western Blot	Cloning and Bacteria Cells Available Making Competent Cells: Various Methods Chemical Transformation Cloning: Various Methods Plasmids NEB sheets for enzyme cutting	General Recipes Buffers Worm Fix 2xDenaturing Dye Spec and Qubit calculators Calculating solutions Calculating protein concentrations
Antibody Work List of Antibodies Available Antibody Storage Antibody Purification Antibody Making/Ordering Cell Culture FISH protocol	Splicing In Vivo Proper cell culture technique In Vivo Cells Available General Cell Culture Methods Harvesting RNA Post-Harvesting RNA PCR primers for splicing Freezing cells In Vitro Background Complex Formation Splicing	Equipment Using the TEM Equipment available