Berglund:Protocols

http://openwetware.org/wiki/Berglund_Lab

Gels Agarose Denaturing Agarose SDS-PAGE Denaturing Acrylamide Native Acrylamide Acrylamide-Agarose Native gels for in vivo splicing	Nucleic Acid PCR: Commonly Used Primers Plasmids PCR: Various Methods Transcription: Various Methods Kinasing Northern Blot Phenol/Ethanol Extraction RNA column purification making rNTP calculating RNA molecular weight	Structure Footprinting UV crosslinking SHAPE (finished protocol) Analytical computer programs
Protein Protein Buffers Protein Purification: Various Methods Binding Gels: Various Methods Protein Concentration Assay: Various Methods Western Blot Coomassie Blue Staining Silverstaining GST pulldown example	Cloning and Bacteria Cells Available Making Competent Cells: Various Methods Chemical Transformation Cloning: Various Methods Plasmids NEB sheets for enzyme cutting	General Recipes Buffers Worm Fix 2xDenaturing Dye Spec and Qubit calculators Calculating solutions Calculating protein concentrations
Antibody Work List of Antibodies Available Antibody Storage Antibody Purification Antibody Making/Ordering	Splicing In Vivo In Vivo Cells Available General Cell Culture Methods Harvesting RNA Post-Harvesting RNA In Vitro Background Complex Formation Splicing	Equipment Using the TEM Equipment available