Berk2006-ConjugationTeam3

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(JCAnderson 22:35, 20 September 2006 (EDT))
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==[[User:JCAnderson|JCAnderson]] 22:35, 20 September 2006 (EDT)==
==[[User:JCAnderson|JCAnderson]] 22:35, 20 September 2006 (EDT)==
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[[Image:JCA092006-gel.jpg|100px|right]]
Four clones were spotted the other day, all 4 were ampS and camS.  The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2.  Clone 1 looks correct, it was stocked and replated to make comp cells.<br>
Four clones were spotted the other day, all 4 were ampS and camS.  The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2.  Clone 1 looks correct, it was stocked and replated to make comp cells.<br>
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[[Image:JCA092006-gel.jpg|100px]]
 
==JL_09/18/06==
==JL_09/18/06==

Revision as of 22:37, 20 September 2006

JCAnderson 22:35, 20 September 2006 (EDT)

Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.

JL_09/18/06

Today I have to:

  1. Grow up J23074 and J23075 (4 clones each)
  2. Grow and spot J23055 (43 degrees J23015 x pCP20)
  3. Run PCR of J23069 with your traG 20mers
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