Berk2006-ConjugationTeam3: Difference between revisions
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==[[User:JCAnderson|JCAnderson]] 22:34, 20 September 2006 (EDT)== | |||
Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells. | |||
[[Image:JCA092006-gel]] | |||
==JL_09/18/06== | ==JL_09/18/06== | ||
Today I have to: | Today I have to: | ||
Revision as of 19:34, 20 September 2006
JCAnderson 22:34, 20 September 2006 (EDT)
Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells. File:JCA092006-gel
JL_09/18/06
Today I have to:
- Grow up J23074 and J23075 (4 clones each)
- Grow and spot J23055 (43 degrees J23015 x pCP20)
- Run PCR of J23069 with your traG 20mers
