Berk2006-ConjugationTeam3: Difference between revisions
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==[[User:JCAnderson|JCAnderson]] 22:35, 20 September 2006 (EDT)== | ==[[User:JCAnderson|JCAnderson]] 22:35, 20 September 2006 (EDT)== | ||
Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells. | Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.<br> | ||
[[Image:JCA092006-gel.jpg]] | [[Image:JCA092006-gel.jpg|100]] | ||
==JL_09/18/06== | ==JL_09/18/06== | ||
Revision as of 19:35, 20 September 2006
JCAnderson 22:35, 20 September 2006 (EDT)
Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.
JL_09/18/06
Today I have to:
- Grow up J23074 and J23075 (4 clones each)
- Grow and spot J23055 (43 degrees J23015 x pCP20)
- Run PCR of J23069 with your traG 20mers
