Berk2006-ConjugationTeam3: Difference between revisions
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==JL_09/22/06== | |||
Today's to do list: | |||
#stock and mini pSB1AG0-J23078, map EcoRI/SpeI | |||
# grow up J23055 #1 for electro comp | |||
#PCR with JL16/JL17 genomic J23016 (trbC::Cm) for the knockout | |||
#Rlam/J23057 x pSB1A2-J01003 redo | |||
==JL_09/20/06== | |||
Today I... | |||
- mini-preped J23074 and J23075, and pSB1AKG0-b0015 | |||
- gen. mini and PCR J23055 | |||
- grew up Rlam/J23057 | |||
- analytical digest of J23074 and J23075 | |||
==[[User:JCAnderson|JCAnderson]] 22:35, 20 September 2006 (EDT)== | ==[[User:JCAnderson|JCAnderson]] 22:35, 20 September 2006 (EDT)== | ||
[[Image:JCA092006-gel.jpg|100px|right]] | [[Image:JCA092006-gel.jpg|100px|right]] | ||
Revision as of 11:12, 22 September 2006
JL_09/22/06
Today's to do list:
- stock and mini pSB1AG0-J23078, map EcoRI/SpeI
- grow up J23055 #1 for electro comp
- PCR with JL16/JL17 genomic J23016 (trbC::Cm) for the knockout
- Rlam/J23057 x pSB1A2-J01003 redo
JL_09/20/06
Today I... - mini-preped J23074 and J23075, and pSB1AKG0-b0015 - gen. mini and PCR J23055 - grew up Rlam/J23057 - analytical digest of J23074 and J23075
JCAnderson 22:35, 20 September 2006 (EDT)
Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.
JL_09/18/06
Today I have to:
- Grow up J23074 and J23075 (4 clones each)
- Grow and spot J23055 (43 degrees J23015 x pCP20)
- Run PCR of J23069 with your traG 20mers
