Berk2006-ConjugationTeam3

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(JCAnderson 22:35, 20 September 2006 (EDT))
(JL_09/22/06)
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#stock and mini pSB1AG0-J23078, map EcoRI/SpeI  
#stock and mini pSB1AG0-J23078, map EcoRI/SpeI  
# grow up J23055 #1 for electro comp
# grow up J23055 #1 for electro comp
-
#PCR with JL16/JL17 genomic J23016 (trbC::Cm)  for the knockout
+
#grow up J23016 (trbC::Cm)  for the knockout (later gen. mini and PCR with JL16/17)
#Rlam/J23057 x pSB1A2-J01003 redo
#Rlam/J23057 x pSB1A2-J01003 redo

Revision as of 15:12, 22 September 2006

Contents

JL_09/22/06

Today's to do list:

  1. stock and mini pSB1AG0-J23078, map EcoRI/SpeI
  2. grow up J23055 #1 for electro comp
  3. grow up J23016 (trbC::Cm) for the knockout (later gen. mini and PCR with JL16/17)
  4. Rlam/J23057 x pSB1A2-J01003 redo

JL_09/20/06

Today I... - mini-preped J23074 and J23075, and pSB1AKG0-b0015 - gen. mini and PCR J23055 - grew up Rlam/J23057 - analytical digest of J23074 and J23075


JCAnderson 22:35, 20 September 2006 (EDT)

Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.

JL_09/18/06

Today I have to:

  1. Grow up J23074 and J23075 (4 clones each)
  2. Grow and spot J23055 (43 degrees J23015 x pCP20)
  3. Run PCR of J23069 with your traG 20mers
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