Berk2006-ConjugationTeam3

JL_09/22/06

Today's to do list:

1. stock and mini pSB1AG0-J23078, map EcoRI/SpeI
2. grow up J23055 #1 for electro comp
3. PCR with JL16/JL17 genomic J23016 (trbC::Cm) for the knockout
4. Rlam/J23057 x pSB1A2-J01003 redo

JL_09/20/06

Today I... - mini-preped J23074 and J23075, and pSB1AKG0-b0015 - gen. mini and PCR J23055 - grew up Rlam/J23057 - analytical digest of J23074 and J23075

JCAnderson 22:35, 20 September 2006 (EDT)

Four clones were spotted the other day, all 4 were ampS and camS. The JL3/4 PCRs were done on genomic preps today. Jen setup and sam ran PCRs of J23055 clones 1 and 2. Clone 1 looks correct, it was stocked and replated to make comp cells.

JL_09/18/06

Today I have to:

1. Grow up J23074 and J23075 (4 clones each)
2. Grow and spot J23055 (43 degrees J23015 x pCP20)
3. Run PCR of J23069 with your traG 20mers