Berk2006-Constructs

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Construction Files

JCA060206-Construction of pAC997
JCA061306-Construction of pSB3C6-J01122
JCA061306-Construction of pSB1A2-J23000
JCA061306-Construction of pSB3C6-J01093
JCA061306-Construction of pSB3C6-J01024
JCA061306-Construction of pSB3C6-J01022
JCA061306-Construction of pSB1A2-J01129X
JCA061606-Construction of key3 library 994
JCA062206-Construction of pSB1A2-J23003
JCA062306-Construction of pJ23004
KAD062806-Construction of J23006-J23007
KAD062806-Construction of J23006
BCH070505-Construction of pJ23006-J23007
BCH070505-Construction of pJ23006-J23008
BCH070505-Construction of pJ23006-J23009
BCH070505-Construction of pJ23006-I13522
SIL070706-Construction of pSB1A2-J23010
BCH071006-Construction of pJ23006-I13522-994Library

SIL071106-Construction of pSB1A2-J23011
JL071106-Construction of pSB1A2-J23012
JL071106-Knockout of oriTF/traM of pOX38
BCH071306-Construction of pSB1A2-J023019
BCH071306-Construction of pSB1A2-J01068
BCH071306-Contruction of pSB3C6-J01068
BCH071806-Construction of pJ23006-J23020
BCH071806-Construction of pJ23006-J23021
BCH071806-Construction of pJ23006-J23022
BCH071806-Construction of pSb1A2-J23023
SIL071906-Construction of pSB1A2-J23013
SIL071906-Construction of pSB1A2-J23014
SIL072006-Construction of J23015
SIL072006-Construction of J23016
SIL072006-Construction of J23017
BCH072006-Construction of pSB1A2-J23018
KAD072106-Construction of pSB1A2-J23029
KAD072106-Construction of pSB1A2-J23024
KAD072106-Construction of pSB1A2-J23025
SIL072506-Construction of pSB1A3-J23026
SIL072506-Construction of pSB1A3-J23027
SIL072506-Construction of pSB1A3-J23028
BCH072506-Construction of pSB3C6-J23018
BCH072506-Construction of pJ23006-J23030
BCH072506-Construction of pJ23006-J23031

BCH072506-Construction of pJ23006-J23032
BCH072506-Construction of pJ23006-J23033
BCH072506-Construction of pJ23006-J23034
BCH072506-Construction of pJ23006-J23035
BCH072506-Construction of pJ23006-J23036
BCH072506-Construction of pSB3C6-J23030
BCH072506-Construction of pSB3C6-J23031
BCH072506-Construction of pSB3C6-J23032
BCH072506-Construction of pSB3C6-J23033
BCH072506-Construction of pSB3C6-J23034
BCH072506-Construction of pSB3C6-J23035
BCH072506-Construction of pSB3C6-J23036
KAD072606-Construction of pSB1A2-J23037
SIL080106-Construction of pSB1A2-J23038
WRB080106-Construction of pSB1A2-J23039
WRB080106-Construction of pSB1A0-J23040
KAD080206-Construction of pSB1A2-J23063
BCH080306-Construction of pSB1A2-J23041
BCH080306-Construction of pSB1A2-J23042
BCH080306-Construction of pSB1A2-J23043
BCH080306-COnstruction of pSB1A2-J23044
JCA080306-COnstruction of pSB3C6-Bca1025 = pSB3C6-J23045
KAD080706-Construction of pSB1A2-J23054
SIL080706-Construction of J23055
SIL080706-Construction of J23056
SIL080706-Construction of J23057
KAD080706-Construction of pSB1A2-J23064
BCH080806-Construction of pJ23006-J23058

BCH080806-Construction of pJ23006-J23059
BCH080806-Construction of pJ23006-J23060
BCH081006-Construction of pJ23006-J23061 key/tRNA
BCH081006-Construction of pJ23006-J23062 key/tRNA
SIL081106-Construction of pSB3C6-J23065
BCH081106-COnstruction of pJ23006-I13500
BCH081106-Construction of pJ23006-J23066
JL081406-Construction of J23067,J23068,J23069 for traG knockout
BCH081606-Construction of pSB3CG0-E1010
JCA082006-Construction of lock3i, lock3j (J23071,J23072)
JCA082006-Construction of lock3k (J23073)
JCA082006-Construction of Pcon (J23119)
JCA082006-Construction of pJ23076 (pSB3C6-Bca1020/r0040)
SIL082406-Construction of pSB3C6-I13521
SIL082506-Construction of pSB1AK3-J23074
SIL082506-Construction of pSB1AK3-J23075
JCA091306-Construction of lock3l (J23077)
JCA091306-Construction of lock3i basic part (J23078)
BCH092106-Construction of pSB3C6-J23079 (J01022 with same RBS spacing as J01122)
BCH092406-Construction of pJ23006-J23081
BCH092406-Construction of pJ23006-J23082, 83, 84 (tRNA homology frameshifts)
BCH092406-Construction of pJ23006-J23085
JCA092906-Construction of pJ23006-J23086
JCA100506-Construction of pSB1AG0-J23088
WRB100806-Construction of pSB1AK3-J23120
WRB100806-Construction of pSB1A2-J23121
WRB100806-construction of pSB1C3-J23121
BCH112106-construction of pJ23006-J23098, 99, 122, 123, 124, 125

SIL112706-Construction of pJ23006-J23201, 202, 203, 204, 205, 206, 207, 208

Construction of J23090 (lock3m variant with wt rbs spacing and extended 5')
Construction of J23093 (lock3n: wt rbs spacing, tight BamHI lock, extended 5')

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