Berk2006-LocksAndKeysTeam: Difference between revisions
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KAD_06/19/06 | KAD_06/19/06 | ||
* Ran analytical gel on PCR products (A (the 994 key3 library) and B (J01093)) made 06/17/06. Visible bands present for each sample confirmed success of PCR. | * Ran analytical gel on PCR products (A (the 994 key3 library) and B (J01093)) made 06/17/06. Visible bands present for each sample confirmed success of PCR. | ||
[[Image:060618gel.jpg|thumb|EIPCR of 994 key3 library]] | |||
* Digested and recircularized 994 key3 library created by PCR. | * Digested and recircularized 994 key3 library created by PCR. | ||
* Transformed 994 key3 library into GeneHogs and TGI with pSB3C6-J01122 by electroboration. Plated products. | * Transformed 994 key3 library into GeneHogs and TGI with pSB3C6-J01122 by electroboration. Plated products. | ||
* Cotransformed J01122 and J01129 by heat shock. Plated product. | * Cotransformed J01122 and J01129 by heat shock. Plated product. | ||
Revision as of 20:55, 19 June 2006
KAD_06/19/06
- Ran analytical gel on PCR products (A (the 994 key3 library) and B (J01093)) made 06/17/06. Visible bands present for each sample confirmed success of PCR.
- Digested and recircularized 994 key3 library created by PCR.
- Transformed 994 key3 library into GeneHogs and TGI with pSB3C6-J01122 by electroboration. Plated products.
- Cotransformed J01122 and J01129 by heat shock. Plated product.
