Berk2006-LocksAndKeysTeam

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KAD_06/19/06

  • Ran analytical gel on PCR products (A (the 994 key3 library) and B (J01093)) made 06/17/06. Visible bands present for each sample confirmed success of PCR.
EIPCR of 994 key3 library
  • Digested and recircularized 994 key3 library created by PCR.
  • Transformed 994 key3 library into GeneHogs and TGI with pSB3C6-J01122 by electroboration. Plated products.
  • Cotransformed J01122 and J01129 by heat shock. Plated product.
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