Berk2006-LocksAndKeysTeam

KAD_06/19/06

• Ran analytical gel on PCR products (A (the 994 key3 library) and B (J01093)) made 06/17/06. Visible bands present for each sample confirmed success of PCR.

EIPCR of 994 key3 library

• Digested and recircularized 994 key3 library created by PCR.
• Transformed 994 key3 library into GeneHogs and TGI with pSB3C6-J01122 by electroboration. Plated products.
• Cotransformed J01122 and J01129 by heat shock. Plated product.

KAD_06/22/06
1. We grew cultures of pSB3C6-J01022, pSB3C6-J01122-A, pSB3C6-J01093, and to saturdation.
2. Put samples in tecan
3. Measured the fluorescence:

• pSB3C6-J01122; at saturation: 20, at midlog: 31
• pSB3C6-J01022; at saturation: 2002, at midlog: 267
• pSB3C6-J01093: at saturation: 24, at midlog: 19
• pSB3C6-J01122/pSBIA2-J01129; at saturation: 2484, at midlog: 311