Berk2006-Oligos: Difference between revisions
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Return to [http://www.openwetware.org/wiki/IGEM:UC_Berkeley/2006 main page] | Return to [http://www.openwetware.org/wiki/IGEM:UC_Berkeley/2006 main page] | ||
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{| border="1" cellpadding="2" <tt> | |||
{| border="1" cellpadding="2" | !width="40"|Name | ||
!width=" | !width="700"|Description | ||
!width=" | !width="200"|Sequence | ||
!width=" | |||
|- | |- | ||
|2005iGEM1 || RlamFwd || caatcacgcgcaccccccggccgttttagcggctaaaaaagtcatgtgtaggctggagctgcttc | |2005iGEM1 || RlamFwd || caatcacgcgcaccccccggccgttttagcggctaaaaaagtcatgtgtaggctggagctgcttc | ||
Line 21: | Line 13: | ||
|- | |- | ||
|2005iGEM4 || TraJ-F-For || ATGTATCCGATGGATCGTATTCAAC | |2005iGEM4 || TraJ-F-For || ATGTATCCGATGGATCGTATTCAAC | ||
|- | |-|2005iGEM5 || TraJF-BBRev || CACGAACTGCAGCGGCCGCTACTAGTAttaacgcgtatttatgatacacatagc | ||
|2005iGEM5 || TraJF-BBRev || CACGAACTGCAGCGGCCGCTACTAGTAttaacgcgtatttatgatacacatagc | |||
|- | |- | ||
|2005iGEM6 || FCtra-rev || CCATAGATATTCCCTGCCCTG | |2005iGEM6 || FCtra-rev || CCATAGATATTCCCTGCCCTG | ||
Line 109: | Line 100: | ||
|- | |- | ||
|2005iGEM48 || BPredForrward || CCTAGGAATTCGCGGCCGCTTCTAGAGATGGCGAGCAGTGAGGACATCATC | |2005iGEM48 || BPredForrward || CCTAGGAATTCGCGGCCGCTTCTAGAGATGGCGAGCAGTGAGGACATCATC | ||
|- | |||
|BBa_G00101 || Reverse sequencing of pSB1A* plasmids || attaccgcctttgagtgagc | |||
|- | |||
|ca850R || Reverse EcoRI for FRT-Kan-Frt at P1 || cacttGAATTCgtgtaggctggagctgcttc | |||
|- | |||
|ca994F || forward Xba1 library for Key3 || CCTATtctagaGACCCAAAAGCANGAGGNNNNNNTAGTNGGTGGTTAATGAAAATTAACTTACTTACTAG | |||
|- | |||
|ca997F || EIPCR for Biobrick p15A/CmR plasmid || ctcgaGGATCCTGCAGctagaaatattttatctgatt | |||
|- | |||
|ca998 || Forward Sequencing of pSB1A2 || gtatcacgaggcagaatttcag | |||
|- | |||
|ca999 || Reverse sequencing of pSB1A2 (fails, don't use) || ccgtattaccgcctttgagtg | |||
|- | |- | ||
|ca1000 || Forward sequencing of pSB3C6 (pAC997) || cagggcagggtcgttaaatagc | |ca1000 || Forward sequencing of pSB3C6 (pAC997) || cagggcagggtcgttaaatagc | ||
Line 114: | Line 117: | ||
|ca1001 || Reverse sequencing of pSB3C6 (pAC997) || ggcggttttttcgttttcagagc | |ca1001 || Reverse sequencing of pSB3C6 (pAC997) || ggcggttttttcgttttcagagc | ||
|- | |- | ||
| | |ca1002F || Forward EcoRI oligo to make XbaI blunter cassette || cttctggaattcgcggccgcaactagtgtccctatcag | ||
|- | |||
|ca1002R || Reverse PstI oligo to make SpeI blunter cassette || gaagcctgcagcggccgctTctagAatataaacgcag | |||
|- | |||
|- | |- | ||
|ca1002F || Forward EcoRI oligo to make XbaI blunter cassette || cttctggaattcgcggccgcaactagtgtccctatcag | |ca1002F || Forward EcoRI oligo to make XbaI blunter cassette || cttctggaattcgcggccgcaactagtgtccctatcag | ||
Line 120: | Line 126: | ||
|ca1002R || Reverse PstI oligo to make SpeI blunter cassette || gaagcctgcagcggccgctTctagAatataaacgcag | |ca1002R || Reverse PstI oligo to make SpeI blunter cassette || gaagcctgcagcggccgctTctagAatataaacgcag | ||
|- | |- | ||
| | |ca1010F || Forward (universal biobrick) EcoRI for FRT || GGACTgaattcgcggccgcttctagag | ||
|- | |||
|ca1010R || Reverse SpeI oligo for FRT || cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg | |||
|- | |||
|ca1016F || Forward EcoRI to biobrick CAT of pKD3 || gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg | |||
|- | |||
|ca1016R || Reverse SpeI to biobrick CAT of pKD3 || ccgctactagtggcgcgcctacctgtgacgg | |||
|- | |||
|ca1020F || Forward SpeI oligo for X-rbsRFP-P plasmid || gcactACTAGTgaaagaggagaaatactagatggc | |||
|- | |||
|ca1020R || Reverse PstI oligo for X-rbsRFP-P plasmid || ccggactgcagcggccgcttctagtatataaacg | |||
|- | |||
|ca1021R || Reverse b0034 XbaI || AGCCATCTAGAATTTCTCCTCTTTCTCTAG | |||
|- | |||
|ca1022R || Reverse SpeI for promoter library || gacttACTAGTAGCTAGCAYWRTMCCTAGGACTGAGCTAGCYRTMARCTCTAGAAGCGGCCGCGAA | |||
|- | |||
|ca1025F || Forward quickchange for GFP[S56AGGA] || gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac | |||
|- | |||
|ca1025R || Reverse quickchange for GFP[S56AGGA] || gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc | |||
|- | |||
|ca1027R || Reverse SpeI to fix lock3 variant rbs spacing || gaagccaactagtatttctcctctt | |||
|- | |||
|ca1028F || Forward XbaI to extend lock3 variants || gcatgTCTAGAGtgtagaccgAACTAGAATCACCTCTTGC | |||
|- | |||
|ca1029R || Reverse oligo for Pcon || gacttActagtaGCTAGCattataCCTAGGactgaGCTAGCtgtcaactctagAAGCGGCCGCGAA | |||
|KB001 || Forward EcoRI to construct Ptet part-making biobrick plasmid || ttctggaattcgcggccgcaactagagccaggcatc | |||
|- | |||
|KB002 || Reverse XbaI to construct Ptet part-making biobrick plasmid || ccgctTctagAagtgctcagtatc | |||
|- | |||
|KB003 || Forward XbaI oligo for part3b || ctccatctagagACCCAAAAGCAAGAGGTGATTCTAGTTtac | |||
|- | |||
|KB004 || Reverse PstI oligo for part3b || gcgaactgcagcggccgctactagtaAACTAGAATCACCTCTTG | |||
|- | |||
|KB005 || Forward XbaI oligo for part 3c || ccatatctagagACCCAAAAGCAAGAGGTGATTCTAGTTggtggttaatgaaaattaacttacttactagaaat | |||
|- | |||
|KB006 || Reverse PstI oligo for part 3c || catctctgcagcggccgctactagtaaagccggattaataatctggctttttagagatatttctagtaagtaagttaa | |||
|- | |||
|KB007 || Forward XbaI oligo for part 3d || TctagAgcgggcgggcgggcgggcgggatccataaaaaaaaaACCCAAAAGCAAGAGGTGATTCTAGTTaaa | |||
|- | |||
|KB008 || Reverse PstI oligo for part 3d || ctgcaggcggccgctactagtacgggcgggcgggcgggcgggaagctttttttttttAACTAGAATCACCTCTT | |||
|- | |||
|KB009 || Forward Ptet screening oligo || tccctatcagtgatagagat | |||
|- | |||
|KB010 || Forward Lock2 screening oligo || aactagaatcacctctagtg | |||
|- | |||
|KB011 || Forward mRFP1 screening oligo || atggcttcctccgaagacgt | |||
|- | |||
|KB012 || KB009 5' add on || CACTGTctagAgcgggcgggcgggc | |||
|- | |||
|KB013 || KB010 3' add on || CCAAActgcaggcggccgctactag | |||
|- | |||
|KB014 || Lock3 4 base add-on to 3' end of hairpin loop forward oligo || GTCGATCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGAC | |||
|- | |||
|KB015 || Lock3 1 base add-on to 3' end of hairpin loop reverse oligo || CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTGTCTTACCCAAAAGCAAGAG | |||
|- | |||
|KB016 || Lock3 2 base add-on to 3' end of hairpin loop reverse oligo || CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTCGTCTTACCCAAAAGCAAGAG | |||
|- | |||
|KB017 || Lock3 4 base add-on to 3' end of hairpin loop reverse oligo || CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTCTGTCTTACCCAAAAGCAAGAG | |||
|- | |||
|KB018 || Lock3 one point mutation on the 5' end of the 3' homology region forward oligo || GTCGATCTAGAGAACTAGAATCAACTCTTGCTTTTGGGTAAGAAAGAGG | |||
|- | |||
|KB019 || Lock3 one point mutation on the 5' end of the 3' homology region reverse oligo || CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTTCTTACCCAAAAGC | |||
|- | |||
|KB020 || Lock3 two consecutive point mutations on the 5' end of the 3' homology region forward oligo || GTCGATCTAGAGAACTAGAATCAATTCTTGCTTTTGGGTAAGAAAGAGG | |||
|- | |||
|KB021 || Lock3 one point mutation on the 3' end of the 3' homology region forward oligo || GTCGATCTAGAGAACTAGAATCACCTCTAGCTTTTGGGTAAGAAAGAGG | |||
|- | |||
|KB022 || Lock3 two consecutive point mutations on the 3' end of the 3' homology region forward oligo || GTCGATCTAGAGAACTAGAATCACCTCAAGCTTTTGGGTAAGAAAGAGG | |||
|- | |||
|JL1 || Forward EcoRI to make spectinomycin resistance biobrick | |||
|| gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc | |||
|- | |||
|JL2 || Reverse SpeI to make spectinomycin resistance biobrick | |||
||ccgctactagtCAGCAACGATGTTACGCAGC | |||
|- | |||
|JL3 ||forward oligo to characterize oriTF/TraM region | |||
|| gatggcattaacccggtaac | |||
|- | |||
|JL4 || reverse oligo to characterize oriTF/TraM region+50bp | |||
|| ctaattatctttcgtactgtc | |||
|- | |||
|JL5 || Forward EcoRI oligo to bioBrick TraM | |||
|| CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc | |||
|- | |||
|JL6 || Reverse SpeI oligo to bioBrick TraM | |||
|| gacttACTAGTgtcaaatttcgtttattcatc | |||
|- | |||
|JL7 || Reverse knockout oligo for oriT/traM | |||
|| cataaaaactctgatatttgaacgaagtcaaatttcgtttATGGGAATTAGCCATGGTCC | |||
|- | |||
|JL8 || Forward oligo to knockout traG-R|| atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag | |||
|- | |||
|JL9 || Reverse oligo to knockout traG-R|| tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg | |||
|- | |||
|JL10 || Forward EcoRI oligo to bioBrick TraG *Internal NotI site|| CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc | |||
|- | |||
|JL11 || Reverse SpeI oligo to bioBrick TraG *internal NotI site|| gacttACTAGTgaagcggctcatatcgtgatc | |||
|- | |||
|JL12 || Forward oligo to knockout trbC|| | |||
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag | |||
|- | |||
|JL13 || Reverse oligo to knockout trbC|| tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatatg | |||
|- | |||
|JL14 || Forward EcoRI oligo to bioBrick trbC *internal PstI site|| CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc | |||
|- | |||
|JL15 || Reverse SpeI oligo to bioBrick trbC *internal PstI site|| gacttACTAGTcgtttcatttcccggaatctc | |||
|- | |||
|JL16 || Forward trbC 20mers || gctggcgttaccagatggtg | |||
|- | |||
|JL17 || Reverse trbC 20mers || gccctgaatactgctgcttcc | |||
|- | |||
|JL18 || Forward TraG 20mers || ctgattatgttggtgcgctg | |||
|- | |||
|Jl19 || Reverse TraG 20mers || cattcgatttccagtacaggc | |||
|- | |||
|JL20 || Forward BglII to BamHI for traG-5' knockout cassette || GAGTCAGATCTgattatgttggtgcgctg | |||
|- | |||
|JL21 || Reverse MfeI to EcoRI for traG-5' knockout cassette || gctggCAATTGcattcgatttccagtacaggc | |||
|- | |||
|JL22 || Reverse EcoRI IPCR of traG || gctgcGAATTCgatactggaaggaatgggc | |||
|- | |||
|JL23 || Forward PstI IPCR of traG || cattgCTGCAGacggcagcattgaagaggcg | |||
|- | |||
|bh001|| forward XbaI oligo for RBS modification of J01022 || gatcgTCTAGAaaagaggagatactagATGgcttcctccgaa | |||
|- | |||
|bh002F|| forward extension oligo to construct J23082 || gatcgggatccATAAAAaagaggtgattctagttcggtct | |||
|- | |||
|bh002R|| reverse extension oligo to construct J23082 || ctagcaagcttTTTTgtgtagaccgaactagaatcacctc | |||
|- | |||
|bh003F|| forward extension oligo to construct J23083 || gatcgggatccATAAAAaaagcaagaggtgattctagttc | |||
|- | |||
|bh003R|| reverse extension oligo to construct J23083 || catcgaagcttTTTTgaccgaactagaatcacctcttgct | |||
|- | |||
|bh004F|| forward extension oligo to construct J23084|| gatcgggatccATAAAAgtcttacccaaaagcaagaggtg | |||
|- | |||
|bh004R|| reverse extension oligo to construct J23084 || cagctaagcttTTTTgaatcacctcttgcttttgggtaag | |||
|- | |||
|SIL01|| Forward BamH oligo to construct J23201 || GTCATggatccataaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL02|| Reverse HindIII oligo to construct J23201 || GATACaagctttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL03|| Forward BamH oligo to construct J23202 || GTCATggatccataaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL04|| Reverse HindIII oligo to construct J23202 || GATACaagctttttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL05|| Forward BamH oligo to construct J23203 || GTCATggatccataaaaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL06|| Reverse HindIII oligo to construct J23203 || GATACaagctttttttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL07|| Forward BamH oligo to construct J23204 || GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL08|| Reverse HindIII oligo to construct J23204 || GATACaagctttttttttttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL09|| Forward BamH oligo to construct J23205 || GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL10|| Reverse HindIII oligo to construct J23205 || GATACaagctttttttttttttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL11|| Forward BamH oligo to construct J23206 || GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL12|| Reverse HindIII oligo to construct J23206 || GATACaagctttttttttttttttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL13|| Forward BamH oligo to construct J23207|| GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL14|| Reverse HindIII oligo to construct J23207 || GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg | |||
|- | |||
|SIL15|| Forward BamH oligo to construct J23208 || GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
|- | |||
|SIL16|| Reverse HindIII oligo to construct J23208 || GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg | |||
|- | |||
|bh005F|| forward extension oligo to construct j23098 || ggctaggatccATAAAAtgtcttacccaaaagcaagaggt | |||
|- | |||
|bh005R|| reverse extension oligo to construct j23098 || ccgataagcttTTTTaatcacctcttgcttttgggtaaga | |||
|- | |||
|bh006F|| forward extension oligo to construct j23099 || ggctaggatccATAAAActgtcttacccaaaagcaagagg | |||
|- | |||
|bh006R|| reverse extension oligo to construct j23099 || ccatgaagcttTTTTatcacctcttgcttttgggtaagac | |||
|- | |||
|bh007F|| forward extension oligo to construct j23122 || ggctaggatccATAAAAtctgtcttacccaaaagcaagag | |||
|- | |||
|bh007R|| reverse extension oligo to construct j23122 || ccgataagcttTTTTtcacctcttgcttttgggtaagaca | |||
|- | |||
|bh008F|| forward extension oligo to construct j23123 || ggctaggatccATAAAAttctgtcttacccaaaagcaaga | |||
|- | |||
|bh008R|| reverse extension oligo to construct j23123 || ccgataagcttTTTTcacctcttgcttttgggtaagacag | |||
|- | |||
|bh009F|| forward extension oligo to construct j230124 || ggctaggatccATAAAAtcttacccaaaagcaagaggtga | |||
|- | |||
|bh009R|| reverse extension oligo to construct j23124 || ccgataagcttTTTTagaatcacctcttgcttttgggtaa | |||
|- | |||
|bh010F|| forward extension oligo to construct j23125 || ggctaggatccATAAAActtacccaaaagcaagaggtgat | |||
|- | |||
|bh010R|| reverse extension oligo to construct j23125 || ccgataagcttTTTTtagaatcacctcttgcttttgggta | |||
|- | |- | ||
| | |oligo|| name || sequence | ||
|} | |} |
Latest revision as of 22:14, 21 November 2006
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Name | Description | Sequence | |||
---|---|---|---|---|---|
2005iGEM1 | RlamFwd | caatcacgcgcaccccccggccgttttagcggctaaaaaagtcatgtgtaggctggagctgcttc | |||
2005iGEM2 | RlamRev | acagcagggaagcagcgcttttccgctgcataaccctgcttcgggatgggaattagccatggtcc | |||
2005iGEM3 | TraJ-F-Rev | TTAACGCGTATTTATGATACACATAGC | |||
2005iGEM4 | TraJ-F-For | ATGTATCCGATGGATCGTATTCAAC | |||
2005iGEM6 | FCtra-rev | CCATAGATATTCCCTGCCCTG | |||
2005iGEM7 | FCtra-fwd | CTCAGTCCTCATGTCTCCGG | |||
2005iGEM8 | OriTBackwardBB | CATTAGAGATTACCTTTTTTGAAC | |||
2005iGEM9 | oriTforwardF | AAGGCTCAACAGGTTGGTGGTTC | |||
2005iGEM10 | TraJF-BBFor | gcagcaGAATTCGCGGCCGCTTCTAGAGatgtatccgatggatcgtattcaac | |||
2005iGEM11 | TraJR-BBRev | CACGAACTGCAGCGGCCGCTACTAGTAtcatggctctgccctcg | |||
2005iGEM12 | TraJR-BBFor | gcagcaGAATTCGCGGCCGCTTCTAGAGatggctgatgaaaccaagcc | |||
2005iGEM13 | FTJiamR | CAGAAAAGACGGAAAGTCTATATTTTATTAACACCTTATTAACGCG ATGGGAATTAGCCATGGTCC | |||
2005iGEM14 | OriT-R-Rev | TTTTCCGCTGCATAACCCTGC | |||
2005iGEM15 | TraJ-R-For | ATGGCTGATGAAAGCAAGCC | |||
2005iGEM16 | TraJ-R-Rev | TCATGGCTCTGCCTCG | |||
2005iGEM17 | OriT-R-For | GCCGCCTTTTCCTCAATCG | |||
2005iGEM18 | FTJiamF | ggtgacagtacgaaagataattagtatattaattacgtggttaat gtgtaggctggagctgcttc | |||
2005iGEM19 | FORlamF | tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc | |||
2005iGEM20 | FORlamR | ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa | |||
2005iGEM21 | tabbR | CTGCAGCGGCCGCTACTAGTAATTATAG... | |||
2005iGEM22 | tabb1R | CTGCAGCGGCCGCTACTAGTATCTGCAG... | |||
2005iGEM23 | tabb2R | CTGCAGCGGCCGCTACTAGTAATAACGT... | |||
2005iGEM24 | crbb1R | CTGCAGCGGCCGCTACTAGTATCTCCTC... | |||
2005iGEM25 | crbb2R | CTGCAGCGGCCGCTACTAGTATCTCCTC... | |||
2005iGEM26 | tabb2F | GAATTCGCGGCCGCTTCTAGAGACCCAA... | |||
2005iGEM27 | crR12wBBR | AGCAGCCTGCAGCGGCCGCTACTAGTAG... | |||
2005iGEM28 | rrnBP1F | GCAGCAGAATTCGCGGCCGCTTCTAGAG... | |||
2005iGEM29 | crbb2F | GAATTCCGGCCGCTTCTAGAGAACTAG... | |||
2005iGEM30 | rrnBP1R | AGCAGCCTGCAGCGGCCGCTACTAGTAG... | |||
2005iGEM31 | crR12wBBF | GCAGCAGAATTCGCGGCCGCTTCTAGAG... | |||
2005iGEM32 | Vlad-fwd | ATACTCGAGATGGCGAGCAGTGAGGACA... | |||
2005iGEM33 | vlad-rev | ATAGAATTCTACAGGAAGAGGTGGTGG | |||
2005iGEM34 | crbb1F | GAATTCGCGGCCGCTTCTAGAGAACTAG... | |||
2005iGEM35 | tabbF | GAATTCGCGGCCGCTTCTAGAGAGAAAA... | |||
2005iGEM36 | tabbF | GAATTCGCGGCCGCTTCTAGAGACCCAA... | |||
2005iGEM37 | ChrrF | GCAGCAGAATTCGCGGCCGCTTCTAGAGATGACGATCCGGCACCATGT | |||
2005iGEM38 | ChrR Reverse | CACGAACTGCAGCGGCCGCTACTAGTATCAGATGCGGAAGAAGGGCT | |||
2005iGEM39 | Predreverse | TCGACTTCTGCAGCGGCCGCTACTAGTA... | |||
2005iGEM40 | RpoEreverse | TCGACTTCTGCAGCGGCCGCTACTAGTATCAACTCATATGCTGGCGCAGACG | |||
2005iGEM41 | gfp_r | GCGGCGAAGCTTTTCCATGCC | |||
2005iGEM42 | gfp_f1 | GCGGCGGGTACCCAGAACT | |||
2005iGEM43 | RpoEforward | CCTAGGAATTCGCGGCCGCTTCTAGAGGTGACGGACAAGAGTGACAGGAC | |||
2005iGEM44 | RpoEp1reverse | TCGACTTCTGCAGCGGCCGCTACTAGTACCGCCCGGAGGTGAAGATAG | |||
2005iGEM45 | RpoEp1forward | CCTAGGAATTCGCGGCCGCTTCTAGAGTTTTTCCCGCCTCCCGCCTG | |||
2005iGEM46 | spoIIEreverse | TCGACTTCTGCAGCGGCCGCTACTAGTATCCTCTCATCTCCCACCTGTTATAT | |||
2005iGEM47 | SpoIIEforward | CCTAGGAATTCGCGGCCGCTTCTAGAGCGGGTCATCCTAACAAATCGGTTTC | |||
2005iGEM48 | BPredForrward | CCTAGGAATTCGCGGCCGCTTCTAGAGATGGCGAGCAGTGAGGACATCATC | |||
BBa_G00101 | Reverse sequencing of pSB1A* plasmids | attaccgcctttgagtgagc | |||
ca850R | Reverse EcoRI for FRT-Kan-Frt at P1 | cacttGAATTCgtgtaggctggagctgcttc | |||
ca994F | forward Xba1 library for Key3 | CCTATtctagaGACCCAAAAGCANGAGGNNNNNNTAGTNGGTGGTTAATGAAAATTAACTTACTTACTAG | |||
ca997F | EIPCR for Biobrick p15A/CmR plasmid | ctcgaGGATCCTGCAGctagaaatattttatctgatt | |||
ca998 | Forward Sequencing of pSB1A2 | gtatcacgaggcagaatttcag | |||
ca999 | Reverse sequencing of pSB1A2 (fails, don't use) | ccgtattaccgcctttgagtg | |||
ca1000 | Forward sequencing of pSB3C6 (pAC997) | cagggcagggtcgttaaatagc | |||
ca1001 | Reverse sequencing of pSB3C6 (pAC997) | ggcggttttttcgttttcagagc | |||
ca1002F | Forward EcoRI oligo to make XbaI blunter cassette | cttctggaattcgcggccgcaactagtgtccctatcag | |||
ca1002R | Reverse PstI oligo to make SpeI blunter cassette | gaagcctgcagcggccgctTctagAatataaacgcag | |||
ca1002F | Forward EcoRI oligo to make XbaI blunter cassette | cttctggaattcgcggccgcaactagtgtccctatcag | |||
ca1002R | Reverse PstI oligo to make SpeI blunter cassette | gaagcctgcagcggccgctTctagAatataaacgcag | |||
ca1010F | Forward (universal biobrick) EcoRI for FRT | GGACTgaattcgcggccgcttctagag | |||
ca1010R | Reverse SpeI oligo for FRT | cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg | |||
ca1016F | Forward EcoRI to biobrick CAT of pKD3 | gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg | |||
ca1016R | Reverse SpeI to biobrick CAT of pKD3 | ccgctactagtggcgcgcctacctgtgacgg | |||
ca1020F | Forward SpeI oligo for X-rbsRFP-P plasmid | gcactACTAGTgaaagaggagaaatactagatggc | |||
ca1020R | Reverse PstI oligo for X-rbsRFP-P plasmid | ccggactgcagcggccgcttctagtatataaacg | |||
ca1021R | Reverse b0034 XbaI | AGCCATCTAGAATTTCTCCTCTTTCTCTAG | |||
ca1022R | Reverse SpeI for promoter library | gacttACTAGTAGCTAGCAYWRTMCCTAGGACTGAGCTAGCYRTMARCTCTAGAAGCGGCCGCGAA | |||
ca1025F | Forward quickchange for GFP[S56AGGA] | gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac | |||
ca1025R | Reverse quickchange for GFP[S56AGGA] | gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc | |||
ca1027R | Reverse SpeI to fix lock3 variant rbs spacing | gaagccaactagtatttctcctctt | |||
ca1028F | Forward XbaI to extend lock3 variants | gcatgTCTAGAGtgtagaccgAACTAGAATCACCTCTTGC | |||
ca1029R | Reverse oligo for Pcon | gacttActagtaGCTAGCattataCCTAGGactgaGCTAGCtgtcaactctagAAGCGGCCGCGAA | KB001 | Forward EcoRI to construct Ptet part-making biobrick plasmid | ttctggaattcgcggccgcaactagagccaggcatc |
KB002 | Reverse XbaI to construct Ptet part-making biobrick plasmid | ccgctTctagAagtgctcagtatc | |||
KB003 | Forward XbaI oligo for part3b | ctccatctagagACCCAAAAGCAAGAGGTGATTCTAGTTtac | |||
KB004 | Reverse PstI oligo for part3b | gcgaactgcagcggccgctactagtaAACTAGAATCACCTCTTG | |||
KB005 | Forward XbaI oligo for part 3c | ccatatctagagACCCAAAAGCAAGAGGTGATTCTAGTTggtggttaatgaaaattaacttacttactagaaat | |||
KB006 | Reverse PstI oligo for part 3c | catctctgcagcggccgctactagtaaagccggattaataatctggctttttagagatatttctagtaagtaagttaa | |||
KB007 | Forward XbaI oligo for part 3d | TctagAgcgggcgggcgggcgggcgggatccataaaaaaaaaACCCAAAAGCAAGAGGTGATTCTAGTTaaa | |||
KB008 | Reverse PstI oligo for part 3d | ctgcaggcggccgctactagtacgggcgggcgggcgggcgggaagctttttttttttAACTAGAATCACCTCTT | |||
KB009 | Forward Ptet screening oligo | tccctatcagtgatagagat | |||
KB010 | Forward Lock2 screening oligo | aactagaatcacctctagtg | |||
KB011 | Forward mRFP1 screening oligo | atggcttcctccgaagacgt | |||
KB012 | KB009 5' add on | CACTGTctagAgcgggcgggcgggc | |||
KB013 | KB010 3' add on | CCAAActgcaggcggccgctactag | |||
KB014 | Lock3 4 base add-on to 3' end of hairpin loop forward oligo | GTCGATCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGAC | |||
KB015 | Lock3 1 base add-on to 3' end of hairpin loop reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTGTCTTACCCAAAAGCAAGAG | |||
KB016 | Lock3 2 base add-on to 3' end of hairpin loop reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTCGTCTTACCCAAAAGCAAGAG | |||
KB017 | Lock3 4 base add-on to 3' end of hairpin loop reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTCTGTCTTACCCAAAAGCAAGAG | |||
KB018 | Lock3 one point mutation on the 5' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCAACTCTTGCTTTTGGGTAAGAAAGAGG | |||
KB019 | Lock3 one point mutation on the 5' end of the 3' homology region reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTTCTTACCCAAAAGC | |||
KB020 | Lock3 two consecutive point mutations on the 5' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCAATTCTTGCTTTTGGGTAAGAAAGAGG | |||
KB021 | Lock3 one point mutation on the 3' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCACCTCTAGCTTTTGGGTAAGAAAGAGG | |||
KB022 | Lock3 two consecutive point mutations on the 3' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCACCTCAAGCTTTTGGGTAAGAAAGAGG | |||
JL1 | Forward EcoRI to make spectinomycin resistance biobrick | gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc | |||
JL2 | Reverse SpeI to make spectinomycin resistance biobrick | ccgctactagtCAGCAACGATGTTACGCAGC | |||
JL3 | forward oligo to characterize oriTF/TraM region | gatggcattaacccggtaac | |||
JL4 | reverse oligo to characterize oriTF/TraM region+50bp | ctaattatctttcgtactgtc | |||
JL5 | Forward EcoRI oligo to bioBrick TraM | CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc | |||
JL6 | Reverse SpeI oligo to bioBrick TraM | gacttACTAGTgtcaaatttcgtttattcatc | |||
JL7 | Reverse knockout oligo for oriT/traM | cataaaaactctgatatttgaacgaagtcaaatttcgtttATGGGAATTAGCCATGGTCC | |||
JL8 | Forward oligo to knockout traG-R | atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag | |||
JL9 | Reverse oligo to knockout traG-R | tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg | |||
JL10 | Forward EcoRI oligo to bioBrick TraG *Internal NotI site | CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc | |||
JL11 | Reverse SpeI oligo to bioBrick TraG *internal NotI site | gacttACTAGTgaagcggctcatatcgtgatc | |||
JL12 | Forward oligo to knockout trbC |
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag | |||
JL13 | Reverse oligo to knockout trbC | tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatatg | |||
JL14 | Forward EcoRI oligo to bioBrick trbC *internal PstI site | CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc | |||
JL15 | Reverse SpeI oligo to bioBrick trbC *internal PstI site | gacttACTAGTcgtttcatttcccggaatctc | |||
JL16 | Forward trbC 20mers | gctggcgttaccagatggtg | |||
JL17 | Reverse trbC 20mers | gccctgaatactgctgcttcc | |||
JL18 | Forward TraG 20mers | ctgattatgttggtgcgctg | |||
Jl19 | Reverse TraG 20mers | cattcgatttccagtacaggc | |||
JL20 | Forward BglII to BamHI for traG-5' knockout cassette | GAGTCAGATCTgattatgttggtgcgctg | |||
JL21 | Reverse MfeI to EcoRI for traG-5' knockout cassette | gctggCAATTGcattcgatttccagtacaggc | |||
JL22 | Reverse EcoRI IPCR of traG | gctgcGAATTCgatactggaaggaatgggc | |||
JL23 | Forward PstI IPCR of traG | cattgCTGCAGacggcagcattgaagaggcg | |||
bh001 | forward XbaI oligo for RBS modification of J01022 | gatcgTCTAGAaaagaggagatactagATGgcttcctccgaa | |||
bh002F | forward extension oligo to construct J23082 | gatcgggatccATAAAAaagaggtgattctagttcggtct | |||
bh002R | reverse extension oligo to construct J23082 | ctagcaagcttTTTTgtgtagaccgaactagaatcacctc | |||
bh003F | forward extension oligo to construct J23083 | gatcgggatccATAAAAaaagcaagaggtgattctagttc | |||
bh003R | reverse extension oligo to construct J23083 | catcgaagcttTTTTgaccgaactagaatcacctcttgct | |||
bh004F | forward extension oligo to construct J23084 | gatcgggatccATAAAAgtcttacccaaaagcaagaggtg | |||
bh004R | reverse extension oligo to construct J23084 | cagctaagcttTTTTgaatcacctcttgcttttgggtaag | |||
SIL01 | Forward BamH oligo to construct J23201 | GTCATggatccataaaacccaaaagcaagaggtgattc | |||
SIL02 | Reverse HindIII oligo to construct J23201 | GATACaagctttttaactagaatcacctcttgcttttg | |||
SIL03 | Forward BamH oligo to construct J23202 | GTCATggatccataaaaaacccaaaagcaagaggtgattc | |||
SIL04 | Reverse HindIII oligo to construct J23202 | GATACaagctttttttaactagaatcacctcttgcttttg | |||
SIL05 | Forward BamH oligo to construct J23203 | GTCATggatccataaaaaaaacccaaaagcaagaggtgattc | |||
SIL06 | Reverse HindIII oligo to construct J23203 | GATACaagctttttttttaactagaatcacctcttgcttttg | |||
SIL07 | Forward BamH oligo to construct J23204 | GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc | |||
SIL08 | Reverse HindIII oligo to construct J23204 | GATACaagctttttttttttttaactagaatcacctcttgcttttg | |||
SIL09 | Forward BamH oligo to construct J23205 | GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
SIL10 | Reverse HindIII oligo to construct J23205 | GATACaagctttttttttttttttaactagaatcacctcttgcttttg | |||
SIL11 | Forward BamH oligo to construct J23206 | GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
SIL12 | Reverse HindIII oligo to construct J23206 | GATACaagctttttttttttttttttaactagaatcacctcttgcttttg | |||
SIL13 | Forward BamH oligo to construct J23207 | GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
SIL14 | Reverse HindIII oligo to construct J23207 | GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg | |||
SIL15 | Forward BamH oligo to construct J23208 | GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | |||
SIL16 | Reverse HindIII oligo to construct J23208 | GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg | |||
bh005F | forward extension oligo to construct j23098 | ggctaggatccATAAAAtgtcttacccaaaagcaagaggt | |||
bh005R | reverse extension oligo to construct j23098 | ccgataagcttTTTTaatcacctcttgcttttgggtaaga | |||
bh006F | forward extension oligo to construct j23099 | ggctaggatccATAAAActgtcttacccaaaagcaagagg | |||
bh006R | reverse extension oligo to construct j23099 | ccatgaagcttTTTTatcacctcttgcttttgggtaagac | |||
bh007F | forward extension oligo to construct j23122 | ggctaggatccATAAAAtctgtcttacccaaaagcaagag | |||
bh007R | reverse extension oligo to construct j23122 | ccgataagcttTTTTtcacctcttgcttttgggtaagaca | |||
bh008F | forward extension oligo to construct j23123 | ggctaggatccATAAAAttctgtcttacccaaaagcaaga | |||
bh008R | reverse extension oligo to construct j23123 | ccgataagcttTTTTcacctcttgcttttgggtaagacag | |||
bh009F | forward extension oligo to construct j230124 | ggctaggatccATAAAAtcttacccaaaagcaagaggtga | |||
bh009R | reverse extension oligo to construct j23124 | ccgataagcttTTTTagaatcacctcttgcttttgggtaa | |||
bh010F | forward extension oligo to construct j23125 | ggctaggatccATAAAActtacccaaaagcaagaggtgat | |||
bh010R | reverse extension oligo to construct j23125 | ccgataagcttTTTTtagaatcacctcttgcttttgggta | |||
oligo | name | sequence |