Berk2006-Oligos: Difference between revisions
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|BBa_G00101 || Reverse sequencing of pSB1A* plasmids || attaccgcctttgagtgagc | |BBa_G00101 || Reverse sequencing of pSB1A* plasmids || attaccgcctttgagtgagc | ||
|- | |- | ||
| ca997F || EIPCR for Biobrick p15A/CmR plasmid || ctcgaGGATCCTGCAGctagaaatattttatctgatt | |ca850R || Reverse EcoRI for FRT-Kan-Frt at P1 || cacttGAATTCgtgtaggctggagctgcttc | ||
|- | |||
|ca994F || forward Xba1 library for Key3 || CCTATtctagaGACCCAAAAGCANGAGGNNNNNNTAGTNGGTGGTTAATGAAAATTAACTTACTTACTAG | |||
|- | |||
|ca997F || EIPCR for Biobrick p15A/CmR plasmid || ctcgaGGATCCTGCAGctagaaatattttatctgatt | |||
|- | |- | ||
|ca998 || Forward Sequencing of pSB1A2 || gtatcacgaggcagaatttcag | |ca998 || Forward Sequencing of pSB1A2 || gtatcacgaggcagaatttcag | ||
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|ca1002R || Reverse PstI oligo to make SpeI blunter cassette || gaagcctgcagcggccgctTctagAatataaacgcag | |ca1002R || Reverse PstI oligo to make SpeI blunter cassette || gaagcctgcagcggccgctTctagAatataaacgcag | ||
|- | |- | ||
|- | |- | ||
| | |ca1002F || Forward EcoRI oligo to make XbaI blunter cassette || cttctggaattcgcggccgcaactagtgtccctatcag | ||
|- | |||
|ca1002R || Reverse PstI oligo to make SpeI blunter cassette || gaagcctgcagcggccgctTctagAatataaacgcag | |||
|- | |||
|ca1010F || Forward (universal biobrick) EcoRI for FRT || GGACTgaattcgcggccgcttctagag | |||
|- | |||
|ca1010R || Reverse SpeI oligo for FRT || cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg | |||
|- | |||
|ca1016F || Forward EcoRI to biobrick CAT of pKD3 || gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg | |||
|- | |||
|ca1016R || Reverse SpeI to biobrick CAT of pKD3 || ccgctactagtggcgcgcctacctgtgacgg | |||
|- | |||
|ca1020F || Forward SpeI oligo for X-rbsRFP-P plasmid || gcactACTAGTgaaagaggagaaatactagatggc | |||
|- | |||
|ca1020R || Reverse PstI oligo for X-rbsRFP-P plasmid || ccggactgcagcggccgcttctagtatataaacg | |||
|- | |||
|ca1021R || Reverse b0034 XbaI || AGCCATCTAGAATTTCTCCTCTTTCTCTAG | |||
|- | |||
|ca1022R || Reverse SpeI for promoter library || gacttACTAGTAGCTAGCAYWRTMCCTAGGACTGAGCTAGCYRTMARCTCTAGAAGCGGCCGCGAA | |||
|- | |||
|ca1025F || Forward quickchange for GFP[S56AGGA] || gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac | |||
|- | |||
|ca1025R || Reverse quickchange for GFP[S56AGGA] || gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc | |||
|- | |- | ||
| | |ca1027R || Reverse SpeI to fix lock3 variant rbs spacing || gaagccaactagtatttctcctctt | ||
|- | |- | ||
| | |ca1028F || Forward XbaI to extend lock3 variants || gcatgTCTAGAGtgtagaccgAACTAGAATCACCTCTTGC | ||
|- | |- | ||
|ca1029R || Reverse oligo for Pcon || gacttActagtaGCTAGCattataCCTAGGactgaGCTAGCtgtcaactctagAAGCGGCCGCGAA | |||
|KB001 || Forward EcoRI to construct Ptet part-making biobrick plasmid || ttctggaattcgcggccgcaactagagccaggcatc | |KB001 || Forward EcoRI to construct Ptet part-making biobrick plasmid || ttctggaattcgcggccgcaactagagccaggcatc | ||
|- | |- |
Revision as of 10:04, 10 September 2006
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Name | Description | Sequence | |||
---|---|---|---|---|---|
2005iGEM1 | RlamFwd | caatcacgcgcaccccccggccgttttagcggctaaaaaagtcatgtgtaggctggagctgcttc | |||
2005iGEM2 | RlamRev | acagcagggaagcagcgcttttccgctgcataaccctgcttcgggatgggaattagccatggtcc | |||
2005iGEM3 | TraJ-F-Rev | TTAACGCGTATTTATGATACACATAGC | |||
2005iGEM4 | TraJ-F-For | ATGTATCCGATGGATCGTATTCAAC | |||
2005iGEM6 | FCtra-rev | CCATAGATATTCCCTGCCCTG | |||
2005iGEM7 | FCtra-fwd | CTCAGTCCTCATGTCTCCGG | |||
2005iGEM8 | OriTBackwardBB | CATTAGAGATTACCTTTTTTGAAC | |||
2005iGEM9 | oriTforwardF | AAGGCTCAACAGGTTGGTGGTTC | |||
2005iGEM10 | TraJF-BBFor | gcagcaGAATTCGCGGCCGCTTCTAGAGatgtatccgatggatcgtattcaac | |||
2005iGEM11 | TraJR-BBRev | CACGAACTGCAGCGGCCGCTACTAGTAtcatggctctgccctcg | |||
2005iGEM12 | TraJR-BBFor | gcagcaGAATTCGCGGCCGCTTCTAGAGatggctgatgaaaccaagcc | |||
2005iGEM13 | FTJiamR | CAGAAAAGACGGAAAGTCTATATTTTATTAACACCTTATTAACGCG ATGGGAATTAGCCATGGTCC | |||
2005iGEM14 | OriT-R-Rev | TTTTCCGCTGCATAACCCTGC | |||
2005iGEM15 | TraJ-R-For | ATGGCTGATGAAAGCAAGCC | |||
2005iGEM16 | TraJ-R-Rev | TCATGGCTCTGCCTCG | |||
2005iGEM17 | OriT-R-For | GCCGCCTTTTCCTCAATCG | |||
2005iGEM18 | FTJiamF | ggtgacagtacgaaagataattagtatattaattacgtggttaat gtgtaggctggagctgcttc | |||
2005iGEM19 | FORlamF | tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc | |||
2005iGEM20 | FORlamR | ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa | |||
2005iGEM21 | tabbR | CTGCAGCGGCCGCTACTAGTAATTATAG... | |||
2005iGEM22 | tabb1R | CTGCAGCGGCCGCTACTAGTATCTGCAG... | |||
2005iGEM23 | tabb2R | CTGCAGCGGCCGCTACTAGTAATAACGT... | |||
2005iGEM24 | crbb1R | CTGCAGCGGCCGCTACTAGTATCTCCTC... | |||
2005iGEM25 | crbb2R | CTGCAGCGGCCGCTACTAGTATCTCCTC... | |||
2005iGEM26 | tabb2F | GAATTCGCGGCCGCTTCTAGAGACCCAA... | |||
2005iGEM27 | crR12wBBR | AGCAGCCTGCAGCGGCCGCTACTAGTAG... | |||
2005iGEM28 | rrnBP1F | GCAGCAGAATTCGCGGCCGCTTCTAGAG... | |||
2005iGEM29 | crbb2F | GAATTCCGGCCGCTTCTAGAGAACTAG... | |||
2005iGEM30 | rrnBP1R | AGCAGCCTGCAGCGGCCGCTACTAGTAG... | |||
2005iGEM31 | crR12wBBF | GCAGCAGAATTCGCGGCCGCTTCTAGAG... | |||
2005iGEM32 | Vlad-fwd | ATACTCGAGATGGCGAGCAGTGAGGACA... | |||
2005iGEM33 | vlad-rev | ATAGAATTCTACAGGAAGAGGTGGTGG | |||
2005iGEM34 | crbb1F | GAATTCGCGGCCGCTTCTAGAGAACTAG... | |||
2005iGEM35 | tabbF | GAATTCGCGGCCGCTTCTAGAGAGAAAA... | |||
2005iGEM36 | tabbF | GAATTCGCGGCCGCTTCTAGAGACCCAA... | |||
2005iGEM37 | ChrrF | GCAGCAGAATTCGCGGCCGCTTCTAGAGATGACGATCCGGCACCATGT | |||
2005iGEM38 | ChrR Reverse | CACGAACTGCAGCGGCCGCTACTAGTATCAGATGCGGAAGAAGGGCT | |||
2005iGEM39 | Predreverse | TCGACTTCTGCAGCGGCCGCTACTAGTA... | |||
2005iGEM40 | RpoEreverse | TCGACTTCTGCAGCGGCCGCTACTAGTATCAACTCATATGCTGGCGCAGACG | |||
2005iGEM41 | gfp_r | GCGGCGAAGCTTTTCCATGCC | |||
2005iGEM42 | gfp_f1 | GCGGCGGGTACCCAGAACT | |||
2005iGEM43 | RpoEforward | CCTAGGAATTCGCGGCCGCTTCTAGAGGTGACGGACAAGAGTGACAGGAC | |||
2005iGEM44 | RpoEp1reverse | TCGACTTCTGCAGCGGCCGCTACTAGTACCGCCCGGAGGTGAAGATAG | |||
2005iGEM45 | RpoEp1forward | CCTAGGAATTCGCGGCCGCTTCTAGAGTTTTTCCCGCCTCCCGCCTG | |||
2005iGEM46 | spoIIEreverse | TCGACTTCTGCAGCGGCCGCTACTAGTATCCTCTCATCTCCCACCTGTTATAT | |||
2005iGEM47 | SpoIIEforward | CCTAGGAATTCGCGGCCGCTTCTAGAGCGGGTCATCCTAACAAATCGGTTTC | |||
2005iGEM48 | BPredForrward | CCTAGGAATTCGCGGCCGCTTCTAGAGATGGCGAGCAGTGAGGACATCATC | |||
BBa_G00101 | Reverse sequencing of pSB1A* plasmids | attaccgcctttgagtgagc | |||
ca850R | Reverse EcoRI for FRT-Kan-Frt at P1 | cacttGAATTCgtgtaggctggagctgcttc | |||
ca994F | forward Xba1 library for Key3 | CCTATtctagaGACCCAAAAGCANGAGGNNNNNNTAGTNGGTGGTTAATGAAAATTAACTTACTTACTAG | |||
ca997F | EIPCR for Biobrick p15A/CmR plasmid | ctcgaGGATCCTGCAGctagaaatattttatctgatt | |||
ca998 | Forward Sequencing of pSB1A2 | gtatcacgaggcagaatttcag | |||
ca999 | Reverse sequencing of pSB1A2 (fails, don't use) | ccgtattaccgcctttgagtg | |||
ca1000 | Forward sequencing of pSB3C6 (pAC997) | cagggcagggtcgttaaatagc | |||
ca1001 | Reverse sequencing of pSB3C6 (pAC997) | ggcggttttttcgttttcagagc | |||
ca1002F | Forward EcoRI oligo to make XbaI blunter cassette | cttctggaattcgcggccgcaactagtgtccctatcag | |||
ca1002R | Reverse PstI oligo to make SpeI blunter cassette | gaagcctgcagcggccgctTctagAatataaacgcag | |||
ca1002F | Forward EcoRI oligo to make XbaI blunter cassette | cttctggaattcgcggccgcaactagtgtccctatcag | |||
ca1002R | Reverse PstI oligo to make SpeI blunter cassette | gaagcctgcagcggccgctTctagAatataaacgcag | |||
ca1010F | Forward (universal biobrick) EcoRI for FRT | GGACTgaattcgcggccgcttctagag | |||
ca1010R | Reverse SpeI oligo for FRT | cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg | |||
ca1016F | Forward EcoRI to biobrick CAT of pKD3 | gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg | |||
ca1016R | Reverse SpeI to biobrick CAT of pKD3 | ccgctactagtggcgcgcctacctgtgacgg | |||
ca1020F | Forward SpeI oligo for X-rbsRFP-P plasmid | gcactACTAGTgaaagaggagaaatactagatggc | |||
ca1020R | Reverse PstI oligo for X-rbsRFP-P plasmid | ccggactgcagcggccgcttctagtatataaacg | |||
ca1021R | Reverse b0034 XbaI | AGCCATCTAGAATTTCTCCTCTTTCTCTAG | |||
ca1022R | Reverse SpeI for promoter library | gacttACTAGTAGCTAGCAYWRTMCCTAGGACTGAGCTAGCYRTMARCTCTAGAAGCGGCCGCGAA | |||
ca1025F | Forward quickchange for GFP[S56AGGA] | gaaacagcatgactttttcaagAGGAgccatgcccgaaggttatgtac | |||
ca1025R | Reverse quickchange for GFP[S56AGGA] | gtacataaccttcgggcatggcTCCTcttgaaaaagtcatgctgtttc | |||
ca1027R | Reverse SpeI to fix lock3 variant rbs spacing | gaagccaactagtatttctcctctt | |||
ca1028F | Forward XbaI to extend lock3 variants | gcatgTCTAGAGtgtagaccgAACTAGAATCACCTCTTGC | |||
ca1029R | Reverse oligo for Pcon | gacttActagtaGCTAGCattataCCTAGGactgaGCTAGCtgtcaactctagAAGCGGCCGCGAA | KB001 | Forward EcoRI to construct Ptet part-making biobrick plasmid | ttctggaattcgcggccgcaactagagccaggcatc |
KB002 | Reverse XbaI to construct Ptet part-making biobrick plasmid | ccgctTctagAagtgctcagtatc | |||
KB003 | Forward XbaI oligo for part3b | ctccatctagagACCCAAAAGCAAGAGGTGATTCTAGTTtac | |||
KB004 | Reverse PstI oligo for part3b | gcgaactgcagcggccgctactagtaAACTAGAATCACCTCTTG | |||
KB005 | Forward XbaI oligo for part 3c | ccatatctagagACCCAAAAGCAAGAGGTGATTCTAGTTggtggttaatgaaaattaacttacttactagaaat | |||
KB006 | Reverse PstI oligo for part 3c | catctctgcagcggccgctactagtaaagccggattaataatctggctttttagagatatttctagtaagtaagttaa | |||
KB007 | Forward XbaI oligo for part 3d | TctagAgcgggcgggcgggcgggcgggatccataaaaaaaaaACCCAAAAGCAAGAGGTGATTCTAGTTaaa | |||
KB008 | Reverse PstI oligo for part 3d | ctgcaggcggccgctactagtacgggcgggcgggcgggcgggaagctttttttttttAACTAGAATCACCTCTT | |||
KB009 | Forward Ptet screening oligo | tccctatcagtgatagagat | |||
KB010 | Forward Lock2 screening oligo | aactagaatcacctctagtg | |||
KB011 | Forward mRFP1 screening oligo | atggcttcctccgaagacgt | |||
KB012 | KB009 5' add on | CACTGTctagAgcgggcgggcgggc | |||
KB013 | KB010 3' add on | CCAAActgcaggcggccgctactag | |||
KB014 | Lock3 4 base add-on to 3' end of hairpin loop forward oligo | GTCGATCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGAC | |||
KB015 | Lock3 1 base add-on to 3' end of hairpin loop reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTGTCTTACCCAAAAGCAAGAG | |||
KB016 | Lock3 2 base add-on to 3' end of hairpin loop reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTCGTCTTACCCAAAAGCAAGAG | |||
KB017 | Lock3 4 base add-on to 3' end of hairpin loop reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTCTGTCTTACCCAAAAGCAAGAG | |||
KB018 | Lock3 one point mutation on the 5' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCAACTCTTGCTTTTGGGTAAGAAAGAGG | |||
KB019 | Lock3 one point mutation on the 5' end of the 3' homology region reverse oligo | CCGATCTGCAGCGGCCGCTACTAGTATCTCCTCTTTCTTACCCAAAAGC | |||
KB020 | Lock3 two consecutive point mutations on the 5' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCAATTCTTGCTTTTGGGTAAGAAAGAGG | |||
KB021 | Lock3 one point mutation on the 3' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCACCTCTAGCTTTTGGGTAAGAAAGAGG | |||
KB022 | Lock3 two consecutive point mutations on the 3' end of the 3' homology region forward oligo | GTCGATCTAGAGAACTAGAATCACCTCAAGCTTTTGGGTAAGAAAGAGG | |||
JL1 | Forward EcoRI to make spectinomycin resistance biobrick | gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc | |||
JL2 | Reverse SpeI to make spectinomycin resistance biobrick | ccgctactagtCAGCAACGATGTTACGCAGC | |||
JL3 | forward oligo to characterize oriTF/TraM region | gatggcattaacccggtaac | |||
JL4 | reverse oligo to characterize oriTF/TraM region+50bp | ctaattatctttcgtactgtc | |||
JL5 | Forward EcoRI oligo to bioBrick TraM | CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc | |||
JL6 | Reverse SpeI oligo to bioBrick TraM | gacttACTAGTgtcaaatttcgtttattcatc | |||
JL7 | Reverse knockout oligo for oriT/traM | cataaaaactctgatatttgaacgaagtcaaatttcgtttATGGGAATTAGCCATGGTCC | |||
JL8 | Forward oligo to knockout traG-R | atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag | |||
JL9 | Reverse oligo to knockout traG-R | tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg | |||
JL10 | Forward EcoRI oligo to bioBrick TraG *Internal NotI site | CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc | |||
JL11 | Reverse SpeI oligo to bioBrick TraG *internal NotI site | gacttACTAGTgaagcggctcatatcgtgatc | |||
JL12 | Forward oligo to knockout trbC |
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag | |||
JL13 | Reverse oligo to knockout trbC | tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatatg | |||
JL14 | Forward EcoRI oligo to bioBrick trbC *internal PstI site | CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc | |||
JL15 | Reverse SpeI oligo to bioBrick trbC *internal PstI site | gacttACTAGTcgtttcatttcccggaatctc | |||
JL16 | Forward trbC 20mers | gctggcgttaccagatggtg | |||
JL17 | Reverse trbC 20mers | gccctgaatactgctgcttcc | |||
JL18 | Forward TraG 20mers | ctgattatgttggtgcgctg | |||
Jl19 | Reverse TraG 20mers | cattcgatttccagtacaggc | |||
JL20 | Forward BglII to BamHI for traG-5' knockout cassette | GAGTCAGATCTgattatgttggtgcgctg | |||
JL21 | Reverse MfeI to EcoRI for traG-5' knockout cassette | gctggCAATTGcattcgatttccagtacaggc | |||
JL22 | Reverse EcoRI IPCR of traG | gctgcGAATTCgatactggaaggaatgggc | |||
JL23 | Forward PstI IPCR of traG | cattgCTGCAGacggcagcattgaagaggcg | |||
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