Berk2006-Oligos: Difference between revisions
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|KB008 || Reverse PstI oligo for part 3d || ctgcaggcggccgctactagtacgggcgggcgggcgggcgggaagctttttttttttAACTAGAATCACCTCTT | |KB008 || Reverse PstI oligo for part 3d || ctgcaggcggccgctactagtacgggcgggcgggcgggcgggaagctttttttttttAACTAGAATCACCTCTT | ||
|- | |||
|JL1 || Forward EcoRI to make spectinomycin resistance biobrick | |||
|| gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc | |||
|- | |||
|JL2 || Reverse SpeI to make spectinomycin resistance biobrick | |||
||ccgctactagtCAGCAACGATGTTACGCAGC | |||
|- | |||
|JL3 ||forward oligo to characterize oriTF/TraM region | |||
|| gatggcattaacccggtaac | |||
|- | |||
|JL4 || reverse oligo to characterize oriTF/TraM region+50bp | |||
|| ctaattatctttcgtactgtc | |||
|- | |||
|JL5 || Forward EcoRI oligo to bioBrick TraM | |||
|| CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc | |||
|- | |||
|JL6 || Reverse SpeI oligo to bioBrick TraM | |||
|| gacttACTAGTgtcaaatttcgtttattcatc | |||
|- | |||
|JL7 || Reverse knockout oligo for oriT/traM | |||
|| cataaaaactctgatatttgaacgaagtcaaatttcgtttATGGGAATTAGCCATGGTCC | |||
|- | |||
|JL8 || Forward oligo to knockout traG-R|| atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag | |||
|- | |||
|JL9 || Reverse oligo to knockout traG-R|| tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg | |||
|- | |||
|JL10 || Forward EcoRI oligo to bioBrick TraG *Internal NotI site|| CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc | |||
|- | |||
|JL11 || Reverse SpeI oligo to bioBrick TraG *internal NotI site|| gacttACTAGTgaagcggctcatatcgtgatc | |||
|- | |||
|JL12 || Forward oligo to knockout trbC|| | |||
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag | |||
|- | |||
|JL13 || Reverse oligo to knockout trbC|| tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatatg | |||
|- | |||
|JL14 || Forward EcoRI oligo to bioBrick trbC *internal PstI site|| CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc | |||
|- | |||
|JL15 || Reverse SpeI oligo to bioBrick trbC *internal PstI site|| gacttACTAGTcgtttcatttcccggaatctc | |||
|} | |} | ||
Revision as of 14:18, 17 July 2006
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| Name | Description | Sequence | Sites |
|---|---|---|---|
| ca997F | EIPCR for Biobrick p15A/CmR plasmid | ctcgaGGATCCTGCAGctagaaatattttatctgatt | BamHI,PstI |
| ca998 | Forward Sequencing of pSB1A2 | gtatcacgaggcagaatttcag | |
| ca999 | Reverse sequencing of pSB1A2 (fails, don't use) | ccgtattaccgcctttgagtg | |
| 2005iGEM1 | RlamFwd | caatcacgcgcaccccccggccgttttagcggctaaaaaagtcatgtgtaggctggagctgcttc | |
| 2005iGEM2 | RlamRev | acagcagggaagcagcgcttttccgctgcataaccctgcttcgggatgggaattagccatggtcc | |
| 2005iGEM3 | TraJ-F-Rev | TTAACGCGTATTTATGATACACATAGC | |
| 2005iGEM4 | TraJ-F-For | ATGTATCCGATGGATCGTATTCAAC | |
| 2005iGEM5 | TraJF-BBRev | CACGAACTGCAGCGGCCGCTACTAGTAttaacgcgtatttatgatacacatagc | |
| 2005iGEM6 | FCtra-rev | CCATAGATATTCCCTGCCCTG | |
| 2005iGEM7 | FCtra-fwd | CTCAGTCCTCATGTCTCCGG | |
| 2005iGEM8 | OriTBackwardBB | CATTAGAGATTACCTTTTTTGAAC | |
| 2005iGEM9 | oriTforwardF | AAGGCTCAACAGGTTGGTGGTTC | |
| 2005iGEM10 | TraJF-BBFor | gcagcaGAATTCGCGGCCGCTTCTAGAGatgtatccgatggatcgtattcaac | |
| 2005iGEM11 | TraJR-BBRev | CACGAACTGCAGCGGCCGCTACTAGTAtcatggctctgccctcg | |
| 2005iGEM12 | TraJR-BBFor | gcagcaGAATTCGCGGCCGCTTCTAGAGatggctgatgaaaccaagcc | |
| 2005iGEM13 | FTJiamR | CAGAAAAGACGGAAAGTCTATATTTTATTAACACCTTATTAACGCG ATGGGAATTAGCCATGGTCC | |
| 2005iGEM14 | OriT-R-Rev | TTTTCCGCTGCATAACCCTGC | |
| 2005iGEM15 | TraJ-R-For | ATGGCTGATGAAAGCAAGCC | |
| 2005iGEM16 | TraJ-R-Rev | TCATGGCTCTGCCTCG | |
| 2005iGEM17 | OriT-R-For | GCCGCCTTTTCCTCAATCG | |
| 2005iGEM18 | FTJiamF | ggtgacagtacgaaagataattagtatattaattacgtggttaat gtgtaggctggagctgcttc | |
| 2005iGEM19 | FORlamF | tttatccgtaaataatttaacccactccacaaaaaggctcaacag gtgtaggctggagctgcttc | |
| 2005iGEM20 | FORlamR | ggaccatggctaattcccat ctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaa | |
| 2005iGEM21 | tabbR | CTGCAGCGGCCGCTACTAGTAATTATAG... | |
| 2005iGEM22 | tabb1R | CTGCAGCGGCCGCTACTAGTATCTGCAG... | |
| 2005iGEM23 | tabb2R | CTGCAGCGGCCGCTACTAGTAATAACGT... | |
| 2005iGEM24 | crbb1R | CTGCAGCGGCCGCTACTAGTATCTCCTC... | |
| 2005iGEM25 | crbb2R | CTGCAGCGGCCGCTACTAGTATCTCCTC... | |
| 2005iGEM26 | tabb2F | GAATTCGCGGCCGCTTCTAGAGACCCAA... | |
| 2005iGEM27 | crR12wBBR | AGCAGCCTGCAGCGGCCGCTACTAGTAG... | |
| 2005iGEM28 | rrnBP1F | GCAGCAGAATTCGCGGCCGCTTCTAGAG... | |
| 2005iGEM29 | crbb2F | GAATTCCGGCCGCTTCTAGAGAACTAG... | |
| 2005iGEM30 | rrnBP1R | AGCAGCCTGCAGCGGCCGCTACTAGTAG... | |
| 2005iGEM31 | crR12wBBF | GCAGCAGAATTCGCGGCCGCTTCTAGAG... | |
| 2005iGEM32 | Vlad-fwd | ATACTCGAGATGGCGAGCAGTGAGGACA... | |
| 2005iGEM33 | vlad-rev | ATAGAATTCTACAGGAAGAGGTGGTGG | |
| 2005iGEM34 | crbb1F | GAATTCGCGGCCGCTTCTAGAGAACTAG... | |
| 2005iGEM35 | tabbF | GAATTCGCGGCCGCTTCTAGAGAGAAAA... | |
| 2005iGEM36 | tabbF | GAATTCGCGGCCGCTTCTAGAGACCCAA... | |
| 2005iGEM37 | ChrrF | GCAGCAGAATTCGCGGCCGCTTCTAGAGATGACGATCCGGCACCATGT | |
| 2005iGEM38 | ChrR Reverse | CACGAACTGCAGCGGCCGCTACTAGTATCAGATGCGGAAGAAGGGCT | |
| 2005iGEM39 | Predreverse | TCGACTTCTGCAGCGGCCGCTACTAGTA... | |
| 2005iGEM40 | RpoEreverse | TCGACTTCTGCAGCGGCCGCTACTAGTATCAACTCATATGCTGGCGCAGACG | |
| 2005iGEM41 | gfp_r | GCGGCGAAGCTTTTCCATGCC | |
| 2005iGEM42 | gfp_f1 | GCGGCGGGTACCCAGAACT | |
| 2005iGEM43 | RpoEforward | CCTAGGAATTCGCGGCCGCTTCTAGAGGTGACGGACAAGAGTGACAGGAC | |
| 2005iGEM44 | RpoEp1reverse | TCGACTTCTGCAGCGGCCGCTACTAGTACCGCCCGGAGGTGAAGATAG | |
| 2005iGEM45 | RpoEp1forward | CCTAGGAATTCGCGGCCGCTTCTAGAGTTTTTCCCGCCTCCCGCCTG | |
| 2005iGEM46 | spoIIEreverse | TCGACTTCTGCAGCGGCCGCTACTAGTATCCTCTCATCTCCCACCTGTTATAT | |
| 2005iGEM47 | SpoIIEforward | CCTAGGAATTCGCGGCCGCTTCTAGAGCGGGTCATCCTAACAAATCGGTTTC | |
| 2005iGEM48 | BPredForrward | CCTAGGAATTCGCGGCCGCTTCTAGAGATGGCGAGCAGTGAGGACATCATC | |
| ca1000 | Forward sequencing of pSB3C6 (pAC997) | cagggcagggtcgttaaatagc | |
| ca1001 | Reverse sequencing of pSB3C6 (pAC997) | ggcggttttttcgttttcagagc | |
| BBa_G00101 | Reverse sequencing of pSB1A* plasmids | attaccgcctttgagtgagc | |
| ca1002F | Forward EcoRI oligo to make XbaI blunter cassette | cttctggaattcgcggccgcaactagtgtccctatcag | |
| ca1002R | Reverse PstI oligo to make SpeI blunter cassette | gaagcctgcagcggccgctTctagAatataaacgcag | |
| ca994F | forward Xba1 library for Key3 | CCTATtctagaGACCCAAAAGCANGAGGNNNNNNTAGTNGGTGGTTAATGAAAATTAACTTACTTACTAG | |
| KB001 | Forward EcoRI to construct Ptet part-making biobrick plasmid | ttctggaattcgcggccgcaactagagccaggcatc | |
| KB002 | Reverse XbaI to construct Ptet part-making biobrick plasmid | ccgctTctagAagtgctcagtatc | |
| KB003 | Forward XbaI oligo for part3b | ctccatctagagACCCAAAAGCAAGAGGTGATTCTAGTTtac | |
| KB004 | Reverse PstI oligo for part3b | gcgaactgcagcggccgctactagtaAACTAGAATCACCTCTTG | |
| KB005 | Forward XbaI oligo for part 3c | ccatatctagagACCCAAAAGCAAGAGGTGATTCTAGTTggtggttaatgaaaattaacttacttactagaaat | |
| KB006 | Reverse PstI oligo for part 3c | catctctgcagcggccgctactagtaaagccggattaataatctggctttttagagatatttctagtaagtaagttaa | |
| KB007 | Forward XbaI oligo for part 3d | TctagAgcgggcgggcgggcgggcgggatccataaaaaaaaaACCCAAAAGCAAGAGGTGATTCTAGTTaaa | |
| KB008 | Reverse PstI oligo for part 3d | ctgcaggcggccgctactagtacgggcgggcgggcgggcgggaagctttttttttttAACTAGAATCACCTCTT | |
| JL1 | Forward EcoRI to make spectinomycin resistance biobrick | gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc | |
| JL2 | Reverse SpeI to make spectinomycin resistance biobrick | ccgctactagtCAGCAACGATGTTACGCAGC | |
| JL3 | forward oligo to characterize oriTF/TraM region | gatggcattaacccggtaac | |
| JL4 | reverse oligo to characterize oriTF/TraM region+50bp | ctaattatctttcgtactgtc | |
| JL5 | Forward EcoRI oligo to bioBrick TraM | CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc | |
| JL6 | Reverse SpeI oligo to bioBrick TraM | gacttACTAGTgtcaaatttcgtttattcatc | |
| JL7 | Reverse knockout oligo for oriT/traM | cataaaaactctgatatttgaacgaagtcaaatttcgtttATGGGAATTAGCCATGGTCC | |
| JL8 | Forward oligo to knockout traG-R | atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag | |
| JL9 | Reverse oligo to knockout traG-R | tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg | |
| JL10 | Forward EcoRI oligo to bioBrick TraG *Internal NotI site | CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc | |
| JL11 | Reverse SpeI oligo to bioBrick TraG *internal NotI site | gacttACTAGTgaagcggctcatatcgtgatc | |
| JL12 | Forward oligo to knockout trbC |
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag | |
| JL13 | Reverse oligo to knockout trbC | tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatatg | |
| JL14 | Forward EcoRI oligo to bioBrick trbC *internal PstI site | CCTAGGAATTCGCGGCCGCTTCTAGatgaagctgagtatgaaatc | |
| JL15 | Reverse SpeI oligo to bioBrick trbC *internal PstI site | gacttACTAGTcgtttcatttcccggaatctc |
