Berk2006-Samantha's Notebook - Revision history

Samanthaliang at 22:40, 11 December 2006

2006-12-11T22:40:53Z

←Older revision		Revision as of 22:40, 11 December 2006
Line 1:		Line 1:
		+	'''12-12-06'''
		+	*Assayed all tRNA-like linker keys
		+	TGI 90
		+	1122 109
		+	1022 13189
		+	J23201 738
		+	J23202 107
		+	J23203 374
		+	J23204 82
		+	J23205 231
		+	J23206 439
		+	J23207 153
		+	J23208 214
		+	J23262 206
		+	J23270 712
		+
		+
	'''12-08-06'''		'''12-08-06'''
	*miniprepped J23202 and transformed in into J01122 (in incubator now)		*miniprepped J23202 and transformed in into J01122 (in incubator now)

Samanthaliang at 08:02, 10 December 2006

2006-12-10T08:02:22Z

←Older revision		Revision as of 08:02, 10 December 2006
Line 2:		Line 2:
	*miniprepped J23202 and transformed in into J01122 (in incubator now)		*miniprepped J23202 and transformed in into J01122 (in incubator now)
	*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)		*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)
-	*Assayed all keys except for J23202, but Tecan was being weird. The following results were the best I could get and were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202. It's weird how the different gains give different results.	+	*Assayed all keys except for J23202
-	TGI ~~1 84~~	+	TGI 93
-	1122 ~~40 59~~	+	1122 89
-	1022 ~~1230 2622~~	+	1022 9765
-	J23201 ~~95 62~~	+	J23201 510
-	J23203 ~~1 484~~	+	J23203 386
-	J23204 ~~52 1~~	+	J23204 281
-	J23205 ~~79 35~~	+	J23205 478
-	J23206 ~~17 375~~	+	J23206 342
-	J23207 ~~92 85~~	+	J23207 323
-	J23208 ~~1 224~~	+	J23208 313
-	*Nevermind ignore above values...will update with correct ones later	+

Samanthaliang at 01:03, 9 December 2006

2006-12-09T01:03:18Z

←Older revision		Revision as of 01:03, 9 December 2006
Line 13:		Line 13:
	J23207 92 85		J23207 92 85
	J23208 1 224		J23208 1 224
		+	*Nevermind ignore above values...will update with correct ones later

Samanthaliang at 00:35, 9 December 2006

2006-12-09T00:35:24Z

←Older revision		Revision as of 00:35, 9 December 2006
Line 2:		Line 2:
	*miniprepped J23202 and transformed in into J01122 (in incubator now)		*miniprepped J23202 and transformed in into J01122 (in incubator now)
	*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)		*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)
-	*Assayed all keys except for J23202, but Tecan was being weird. The following results were the best I could get and were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202.	+	*Assayed all keys except for J23202, but Tecan was being weird. The following results were the best I could get and were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202. It's weird how the different gains give different results.
	TGI 1 84		TGI 1 84
	1122 40 59		1122 40 59

Samanthaliang at 00:34, 9 December 2006

2006-12-09T00:34:23Z

←Older revision		Revision as of 00:34, 9 December 2006
Line 2:		Line 2:
	*miniprepped J23202 and transformed in into J01122 (in incubator now)		*miniprepped J23202 and transformed in into J01122 (in incubator now)
	*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)		*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)
-	*Assayed all keys except for J23202, but Tecan was being ~~wierd and wouldn't give good numbers even though I changed both the integration time and the gain like 20 times~~. The following results were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202.	+	*Assayed all keys except for J23202, but Tecan was being weird. The following results were the best I could get and were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202.
	TGI 1 84		TGI 1 84
	1122 40 59		1122 40 59

Samanthaliang at 00:26, 9 December 2006

2006-12-09T00:26:45Z

←Older revision		Revision as of 00:26, 9 December 2006
Line 2:		Line 2:
	*miniprepped J23202 and transformed in into J01122 (in incubator now)		*miniprepped J23202 and transformed in into J01122 (in incubator now)
	*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)		*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)
-	*Assayed all keys except for J23202, but Tecan was being wierd and wouldn't give good numbers even though I changed both the integration time and the gain like 20 times. The following results were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s ~~rather than the 10000s~~. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202.	+	*Assayed all keys except for J23202, but Tecan was being wierd and wouldn't give good numbers even though I changed both the integration time and the gain like 20 times. The following results were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202.
	TGI 1 84		TGI 1 84
	1122 40 59		1122 40 59

Samanthaliang at 00:21, 9 December 2006

2006-12-09T00:21:46Z

←Older revision		Revision as of 00:21, 9 December 2006
Line 13:		Line 13:
	J23207 92 85		J23207 92 85
	J23208 1 224		J23208 1 224
		+
		+

	'''12-06-06'''		'''12-06-06'''

Samanthaliang at 00:21, 9 December 2006

2006-12-09T00:21:19Z

←Older revision		Revision as of 00:21, 9 December 2006
Line 2:		Line 2:
	*miniprepped J23202 and transformed in into J01122 (in incubator now)		*miniprepped J23202 and transformed in into J01122 (in incubator now)
	*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)		*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)
-	*Assayed all keys except for J23202, but Tecan was being wierd and wouldn't give good numbers even though I changed both the integration time and the gain like 20 times. The following results were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s rather than the 10000s. Might want to repeat this assay sometime.	+	*Assayed all keys except for J23202, but Tecan was being wierd and wouldn't give good numbers even though I changed both the integration time and the gain like 20 times. The following results were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s rather than the 10000s. Note the keys are all transformed into J01122. Might want to repeat this assay sometime with j23202.
	TGI 1 84		TGI 1 84
	1122 40 59		1122 40 59

Samanthaliang at 00:20, 9 December 2006

2006-12-09T00:20:29Z

←Older revision		Revision as of 00:20, 9 December 2006
Line 1:		Line 1:
		+	'''12-08-06'''
		+	*miniprepped J23202 and transformed in into J01122 (in incubator now)
		+	*sequenced J23202, J23204, J23205, J23206, J23207, and J23208 (check J23205 and J23207 carefully)
		+	*Assayed all keys except for J23202, but Tecan was being wierd and wouldn't give good numbers even though I changed both the integration time and the gain like 20 times. The following results were taken at a gain of 180 and 200 with the max integration time, and the OnRFP is still only in the 1000s rather than the 10000s. Might want to repeat this assay sometime.
		+	TGI 1 84
		+	1122 40 59
		+	1022 1230 2622
		+	J23201 95 62
		+	J23203 1 484
		+	J23204 52 1
		+	J23205 79 35
		+	J23206 17 375
		+	J23207 92 85
		+	J23208 1 224
		+
	'''12-06-06'''		'''12-06-06'''
	*sequencing results for the old keys came out nicely - J23201 & J23203 were perfect, but J23205 and J23207 were switched. Not too sure if the minipreps are actually reversed or if I just messed up while sequencing...any way to do figure out besides sequencing again?		*sequencing results for the old keys came out nicely - J23201 & J23203 were perfect, but J23205 and J23207 were switched. Not too sure if the minipreps are actually reversed or if I just messed up while sequencing...any way to do figure out besides sequencing again?

Samanthaliang at 00:25, 7 December 2006

2006-12-07T00:25:27Z

←Older revision		Revision as of 00:25, 7 December 2006
Line 1:		Line 1:
		+	'''12-06-06'''
		+	*sequencing results for the old keys came out nicely - J23201 & J23203 were perfect, but J23205 and J23207 were switched. Not too sure if the minipreps are actually reversed or if I just messed up while sequencing...any way to do figure out besides sequencing again?
		+	*Assuming all the even keys are most likely right as well (will sequence them later), I transformed all the keys into J01122 (lock3) except for J23202 because the miniprep has disappeared for some reason
		+	*Grew up a culture of J23202 to miniprep - will send all the even keys out for sequencing once I get J23202.
		+
		+
		+
	'''12-01-06'''		'''12-01-06'''
	*a little scared that the backbone digest of J23061 may have been wierd because Chris has been getting wierd results with it		*a little scared that the backbone digest of J23061 may have been wierd because Chris has been getting wierd results with it