Berk2006-Sequences: Difference between revisions

Revision as of 12:20, 23 August 2006

Sequence files in ApE format

download ApE

File:PAC997.str
File:PSB1A2.str
File:PSB1A3.str
File:PSB1AK3.str

#F54D70 #5DFC0A #48D1CC #FF5333 #CC00FF #EEC900

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To Do<o:p></o:p>

Ogel MC600u x M1/P1 (2 clones), pBACr-90* x MG984, pMF19, MG1655 x MC600u/P1 (2 clones plus dust?)<o:p></o:p>

<o:p> </o:p>

Comp MGu x UG874<o:p></o:p>

<o:p> </o:p>

sub pSB3C6-Bca1020 (for r0040)<o:p></o:p>

Sub pMP908<o:p></o:p>

<o:p> </o:p>

Sub new locks<o:p></o:p>

Sub new promoter J23119<o:p></o:p>

<o:p> </o:p>

Grow to assay J23066, x locks (<st1:time Minute="30" Hour="17"><span style='color:windowtext;

text-decoration:none;text-underline:none'>5:30</span></span></st1:time><span

class=MsoHyperlink>)<o:p></o:p>

Stock, seq 62, M1, UG874<o:p></o:p>

Outgrow UG874 x MC600u<o:p></o:p>

<o:p> </o:p>

Riboregulators<o:p></o:p>

To do: keys that anneal further upstream, repairing the rbs in lock, cytometry on the above.<o:p></o:p>

Make pSB3C6-I13521<o:p></o:p>

<o:p> </o:p>

Conjugation<o:p></o:p>

Screen the trbC mutants by PCR/phenotype, clear pKD46<o:p></o:p>

oriTf mutant x 1064, test phenotype<o:p></o:p>

Rlam traG mutant, screen colonies<o:p></o:p>

trbC/traG complementation plasmids (Pcon-rbs is good, maybe need to reprep)<o:p></o:p>

<o:p> </o:p>

<o:p> </o:p>

<o:p> </o:p>

LB + 5 mM CaCl2 and 0.2% glucose<o:p></o:p>

LB + 100 mM MgSO4 + 5 mM CaCl2<o:p></o:p>

200 µL 1 M Na-Citrate (pH 5.5)<o:p></o:p>

chloroform<o:p></o:p>

<o:p> </o:p>

Berk prop/email adam<o:p></o:p>

Meet w/ Wendell,Shaffer,other bioE<o:p></o:p>

<o:p> </o:p>

Solid phase on plate<o:p></o:p>

Sequence pCP20, clone it into new pMP plasmid<o:p></o:p>

UCSF Safety<o:p></o:p>

K99<o:p></o:p>

Mouse serum/Balb/c<o:p></o:p>

Venter<o:p></o:p>

<o:p> </o:p>

########### #########################<o:p></o:p>

<o:p> </o:p>

<o:p> </o:p>

Inject BO828/pBACr-CyoWbb / pAC-TetInv / pBR-GFP into tumors, tetR library, fdhF-tetR inverter<o:p></o:p>

<o:p> </o:p>

Email Jamie for protocols<o:p></o:p>

Email Jack about tailvein injections<o:p></o:p>

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Berk2006-Sequences: Difference between revisions

Revision as of 12:20, 23 August 2006

Sequence files in ApE format

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