Berk2006-StockBoxes

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(Box2)
 
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'''Procedure:''' Combine equal volumes of cells and glycerol (50% cells, 50%glycerol).  Freeze in cryo vial.
'''Procedure:''' Combine equal volumes of cells and glycerol (50% cells, 50%glycerol).  Freeze in cryo vial.
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'''Box1<br>'''
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==Box1==
{| border="1" cellpadding="2"
{| border="1" cellpadding="2"
!width="10"|A
!width="10"|A
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|J01001||J01002||J01003||J01109||J01021||J01022||J01023||J01124||J01126||
|J01001||J01002||J01003||J01109||J01021||J01022||J01023||J01124||J01126||
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|-
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|J01127||J01066||pSB3C6-J01022-B||BW20767|| ||-||pSB3C6-J01093 (TGI/Clone 1)||pSB3C6-J01024 (TGI/Clone 1)|| TG1 ||
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|J01127||J01066||pSB3C6-J01022-B||BW20767||pJ23006-J23009 ||-||pSB3C6-J01093 (TGI/Clone 1)||pSB3C6-J01024 (TGI/Clone 1)|| TG1 ||
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||pJ23006-I13522||pJ23006-J23007||pJ23006-J23008||pSB1A2-J23012||||pSB1A2-J23018-2||"pSB1A2"-J01007||"pSB1A2"-J01006|||
||pJ23006-I13522||pJ23006-J23007||pJ23006-J23008||pSB1A2-J23012||||pSB1A2-J23018-2||"pSB1A2"-J01007||"pSB1A2"-J01006|||
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|-
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||pJ23006-J23030-1||pJ23006-J23031-1||pJ23006-J23032-1||pJ23006-J23033-1||pJ23006-J23034-3||pJ23006-J23035-1||pJ23006-J23035-1|||||
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||pJ23006-J23030-1||pJ23006-J23031-1||pJ23006-J23032-1||pJ23006-J23033-1||pJ23006-J23034-3||pJ23006-J23035-1||pJ23006-J23036-1||pJ23006-J23043-1|||
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||pJ23006-J23044-1||pJ23006-J23022-1||pJ23006-J23070-1||pJ23006-J23066-1||pJ23006-J23062(trna key)-1||pSB1AT3||J23087 (dOriTf,dTrbC)||J23089 (dOriTr,dTraG)|||
|}
|}
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==Box2==
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'''Box2<br>'''
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{| border="1" cellpadding="2"
{| border="1" cellpadding="2"
!width="10"|A
!width="10"|A
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|pSpoIIE Amp||pSpoIIE kan||pSB1A2-I13522 in JM109||pSB1A2-S03155||994 Library 06/27/06||pSB1A2-T9002 in TGI||pSB1A2-P0412 in TGI||pSB1A2-I13603 in TGI||pSB1A2-F2620 in TGI||
|pSpoIIE Amp||pSpoIIE kan||pSB1A2-I13522 in JM109||pSB1A2-S03155||994 Library 06/27/06||pSB1A2-T9002 in TGI||pSB1A2-P0412 in TGI||pSB1A2-I13603 in TGI||pSB1A2-F2620 in TGI||
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|-
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|pSB1A2-I13601 in TGI||pSB1AK3-F1610 in TGI||pSB1A2-R0040+F1610#1_07/25/06||pSB1A2-F2620+P0412#1_07/25/06||J23016 (pOX38 trbCf KO)||J23015 (pOX38 OriT KO)||||||||||
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|pSB1A2-I13601 in TGI||pSB1AK3-F1610 in TGI||pSB1A2-R0040+F1610#1_07/25/06||pSB1A2-F2620+P0412#1_07/25/06||J23016 (pOX38 trbCf KO)CamR||J23015 (pOX38 OriT KO)CamR||J23056 (CamS pOX38 trbCf KO)-1||J23056 (CamS pOX38 trbCf KO)-2||||||
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|[lock 3n] J23093||pSB1A2-J23038 #4||pJ23006-J23061||pSB3C6-J23090 #1||||J23039 || J23040 ||pSB3C6-J23071-2||pSB3C6-J23072-2||
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|pJ23019-J23072 #5||J23057 (TraG KO clone#2)||pBca1020-J23038 #2||pSB3C6-J23077 #1||pSB1A2-J23078 #1||J23055 #1 (JCA_092006)||psB1AG0-J23078||||||
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|pSB3C6-Bca9109-2|| pSB3C6-Bca9114-1||pSB3C6-Bca9114-2||pSB3C6-Bca9115-1||pSB3C6-Bca9115-2||||||||||
|}
|}
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'''Box3<br>'''
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==Box3==
{| border="1" cellpadding="2"
{| border="1" cellpadding="2"
!width="10"|A
!width="10"|A
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|-
|| pBca1020-Bca1022 lib hit F12 || pBca1020-Bca1022 lib hit A2 || pBca1020-Bca1022 lib hit E8  
|| pBca1020-Bca1022 lib hit F12 || pBca1020-Bca1022 lib hit A2 || pBca1020-Bca1022 lib hit E8  
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|| pBca1020-Bca1022 lib A || pBca1020-Bca1022 lib B ||  || pBca1020-Bca1022 lib D || || ||
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|| pBca1020-Bca1022 lib A || pBca1020-Bca1022 lib B ||  || pBca1020-Bca1022 lib D || pJ23076 in TG1 || pSB1A2-J23119 ||
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||pSB3C6-Bca1025-1||pSB3C6-Bca1025-2|||||||||||||||
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||pSB3C6-Bca1025-1||pSB3C6-Bca1025-2||psB1A2-Bca1041||pJ23006-Bca9094||pSB1A2-Bca1061-1 ||pSB3CG0-Bca9075 ||pJ23006-Bca1058-1 ||pJ23006-Bca9103-2|||
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||pJ23006-Bca1059-1||pJ23006-Bca1059-2||pJ23006-Bca1062-1||pJ23006-Bca1062-2||pSB1A2-Bca1072-1||pSB1A2-Bca1072-2||pSB1A2-Bca1073-1||pSB1A2-Bca1073-2|||
|}
|}

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Procedure: Combine equal volumes of cells and glycerol (50% cells, 50%glycerol). Freeze in cryo vial.

Box1

A B C D E F G H I
J01004J01008J01063J01028J01064J01021J01022J01023J01024(KAN
J01020J01030J01099J01100J01027J01052J01055J01050J01060
J01057J01058J01059J01067J01068J01076J01076J01075J01093
J01092J01094J01096J01098J01129/MC1061J01002/MC1061J01093/MC1061pSB3C6-J01122/TGIpAC997-1/MC1061
J01001J01002J01003J01109J01021J01022J01023J01124J01126
J01127J01066pSB3C6-J01022-BBW20767pJ23006-J23009 -pSB3C6-J01093 (TGI/Clone 1)pSB3C6-J01024 (TGI/Clone 1) TG1
pJ23006-I13522pJ23006-J23007pJ23006-J23008pSB1A2-J23012pSB1A2-J23018-2"pSB1A2"-J01007"pSB1A2"-J01006
pJ23006-J23030-1pJ23006-J23031-1pJ23006-J23032-1pJ23006-J23033-1pJ23006-J23034-3pJ23006-J23035-1pJ23006-J23036-1pJ23006-J23043-1
pJ23006-J23044-1pJ23006-J23022-1pJ23006-J23070-1pJ23006-J23066-1pJ23006-J23062(trna key)-1pSB1AT3J23087 (dOriTf,dTrbC)J23089 (dOriTr,dTraG)

Box2

A B C D E F G H I
LOCK2ID500IL2007R0040R0051ROO80 pSB2K3PK3PK2E0420 pSB1A2
E0430E084013521 SB1A3I3006E0024E1010 pSB2K3C0051C0040 tetRB0015
BBa_B0020 RBS strongB0034E6030 pSB1Ak3oriTF0lambdaoriTRoriTRlock1traJR
oriTF on RFPJ01020J01021TlambdaOlambdaRlambdaT6J01024J01022J01023
pSpoIIE AmppSpoIIE kanpSB1A2-I13522 in JM109pSB1A2-S03155994 Library 06/27/06pSB1A2-T9002 in TGIpSB1A2-P0412 in TGIpSB1A2-I13603 in TGIpSB1A2-F2620 in TGI
pSB1A2-I13601 in TGIpSB1AK3-F1610 in TGIpSB1A2-R0040+F1610#1_07/25/06pSB1A2-F2620+P0412#1_07/25/06J23016 (pOX38 trbCf KO)CamRJ23015 (pOX38 OriT KO)CamRJ23056 (CamS pOX38 trbCf KO)-1J23056 (CamS pOX38 trbCf KO)-2
[lock 3n] J23093pSB1A2-J23038 #4pJ23006-J23061pSB3C6-J23090 #1J23039 J23040 pSB3C6-J23071-2pSB3C6-J23072-2
pJ23019-J23072 #5J23057 (TraG KO clone#2)pBca1020-J23038 #2pSB3C6-J23077 #1pSB1A2-J23078 #1J23055 #1 (JCA_092006)psB1AG0-J23078
pSB3C6-Bca9109-2 pSB3C6-Bca9114-1pSB3C6-Bca9114-2pSB3C6-Bca9115-1pSB3C6-Bca9115-2

Box3

A B C D E F G H I
pUC19pKD46-1pKD13pKD46-2RP4 (Amp,Kan,Tet)R35:tetR2:1 RBS:tetR2:1 RBS:cIRP1 x K1200 Kan
RP1 xK1200 NDRP4 (Amp,Kan,Tet)Top10RP1 xK1200 Ampp0X38 Kan (F)PCVtop10Frost FR0040
B0015E0430 pSB1A2I05115spo0A topo 1:1spo0A topo 1:2Terminator BBa_B0015spoIIE topo 1:12:2 RBS:cIi51840
E0030 RBS strongR0040 pSB1A2I0500spoIIE topo 1:2E0840C0051 cI reppSpoIIa + YFPI12007 pSB2K3pKD46 in DH10B
pKD46 x RlambdapKD46 x pOX38 pBca1020-Bca1022 lib hit C2 pBca1020-Bca1022 lib hit D7 pBca1020-Bca1022 lib hit C5 pBca1020-Bca1022 lib hit H12 pBca1020-Bca1022 lib hit E1 pBca1020-Bca1022 lib hit B10 pBca1020-Bca1022 lib hit E11
pBca1020-Bca1022 lib hit F3 pBca1020-Bca1022 lib hit A5 pBca1020-Bca1022 lib hit F6 pBca1020-Bca1022 lib hit E10 pBca1020-Bca1022 lib hit C1 pBca1020-Bca1022 lib hit B9 pBca1020-Bca1022 lib hit F4 pBca1020-Bca1022 lib hit D7 pBca1020-Bca1022 lib hit C2
pBca1020-Bca1022 lib hit F12 pBca1020-Bca1022 lib hit A2 pBca1020-Bca1022 lib hit E8 pBca1020-Bca1022 lib A pBca1020-Bca1022 lib B pBca1020-Bca1022 lib D pJ23076 in TG1 pSB1A2-J23119
pSB3C6-Bca1025-1pSB3C6-Bca1025-2psB1A2-Bca1041pJ23006-Bca9094pSB1A2-Bca1061-1 pSB3CG0-Bca9075 pJ23006-Bca1058-1 pJ23006-Bca9103-2
pJ23006-Bca1059-1pJ23006-Bca1059-2pJ23006-Bca1062-1pJ23006-Bca1062-2pSB1A2-Bca1072-1pSB1A2-Bca1072-2pSB1A2-Bca1073-1pSB1A2-Bca1073-2

Box4: TlambdaOlambda F plasmid KCM competent cells.

Box5: Rlambda R plasmid KCM competent cells.

Box6: Glycerol Stocks of EC100D::pir+ and pOX38

Box7: pSB3C6-J01122 KCM Competent cells (orange and green stripes).

Box 8: TG1

Back Compartment: Olambda F plasmid (OriT knockout) KCM competent cells.

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