Berk2006-StockBoxes

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Procedure: Combine equal volumes of cells and glycerol (50% cells, 50%glycerol). Freeze in cryo vial.

Box1

A	B	C	D	E	F	G	H	I
J01004	J01008	J01063	J01028	J01064	J01021	J01022	J01023	J01024(KAN
J01020	J01030	J01099	J01100	J01027	J01052	J01055	J01050	J01060
J01057	J01058	J01059	J01067	J01068	J01076	J01076	J01075	J01093
J01092	J01094	J01096	J01098	J01129/MC1061	J01002/MC1061	J01093/MC1061	pSB3C6-J01122/TGI	pAC997-1/MC1061
J01001	J01002	J01003	J01109	J01021	J01022	J01023	J01124	J01126
J01127	J01066	pSB3C6-J01022-B	BW20767	pJ23006 #1 in TG1 (jca)	-	pSB3C6-J01093 (TGI/Clone 1)	pSB3C6-J01024 (TGI/Clone 1)	TG1
pJ23006-I13522 version number one. delete this comment when sequence data confirms accuracy.	pJ23006-J23007	pJ23006-J23008

Box2

A	B	C	D	E	F	G	H	I
LOCK2	ID500	IL2007	R0040	R0051	ROO80 pSB2K3	PK3	PK2	E0420 pSB1A2
E0430	E0840	13521 SB1A3	I3006	E0024	E1010 pSB2K3	C0051	C0040 tetR	B0015
BBa_B0020 RBS strong	B0034	E6030 pSB1Ak3	oriTF	0lambda	oriTR	oriTR	lock1	traJR
oriTF on RFP	J01020	J01021	TlambdaOlambda	Rlambda	T6	J01024	J01022	J01023
pSpoIIE Amp	pSpoIIE kan	pSB1A2-I13522 in JM109	pSB1A2-S03155	994 Library 06/27/06

Box3

A	B	C	D	E	F	G	H	I
pUC19	pKD46-1	pKD13	pKD46-2	RP4 (Amp,Kan,Tet)	R35:tetR	2:1 RBS:tetR	2:1 RBS:cI	RP1 x K1200 Kan
RP1 xK1200 ND	RP4 (Amp,Kan,Tet)	Top10	RP1 xK1200 Amp	pK38 Kan (F)	PCV	top10	Frost F	R0040
B0015	E0430 pSB1A2	I05115	spo0A topo 1:1	spo0A topo 1:2	Terminator BBa_B0015	spoIIE topo 1:1	2:2 RBS:cI	i51840
E0030 RBS strong	R0040 pSB1A2	I0500	spoIIE topo 1:2	E0840	C0051 cI rep	pSpoIIa + YFP	I12007 pSB2K3	pKD46 in DH10B

Box4: TlambdaOlambda F plasmid KCM competent cells.

Box5: Rlambda R plasmid KCM competent cells.

Box6: Glycerol Stocks of EC100D::pir+ and pOX38

Box7: pSB3C6-J01122 KCM Competent cells (orange and green stripes).

Box 8: TG1

Back Compartment: Olambda F plasmid (OriT knockout) KCM competent cells.