Berk2006-StockBoxes

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Procedure: Combine equal volumes of cells and glycerol (50% cells, 50%glycerol). Freeze in cryo vial.

Box1

A B C D E F G H I
J01004J01008J01063J01028J01064J01021J01022J01023J01024(KAN
J01020J01030J01099J01100J01027J01052J01055J01050J01060
J01057J01058J01059J01067J01068J01076J01076J01075J01093
J01092J01094J01096J01098J01129/MC1061J01002/MC1061J01093/MC1061pSB3C6-J01122/TGIpAC997-1/MC1061
J01001J01002J01003J01109J01021J01022J01023J01124J01126
J01127J01066pSB3C6-J01022-BBW20767 -pSB3C6-J01093 (TGI/Clone 1)pSB3C6-J01024 (TGI/Clone 1) TG1
pJ23006-I13522pJ23006-J23007pJ23006-J23008pSB1A2-J23012pSB1A2-J23018-2"pSB1A2"-J01007"pSB1A2"-J01006


Box2

A B C D E F G H I
LOCK2ID500IL2007R0040R0051ROO80 pSB2K3PK3PK2E0420 pSB1A2
E0430E084013521 SB1A3I3006E0024E1010 pSB2K3C0051C0040 tetRB0015
BBa_B0020 RBS strongB0034E6030 pSB1Ak3oriTF0lambdaoriTRoriTRlock1traJR
oriTF on RFPJ01020J01021TlambdaOlambdaRlambdaT6J01024J01022J01023
pSpoIIE AmppSpoIIE kanpSB1A2-I13522 in JM109pSB1A2-S03155994 Library 06/27/06pSB1A2-T9002 in TGIpSB1A2-P0412 in TGIpSB1A2-I13603 in TGIpSB1A2-F2620 in TGI
pSB1A2-I13601 in TGIpSB1AK3-F1610 in TGIpSB1A2-R0040+F1610#1_07/25/06pSB1A2-F2620+P0412#1_07/25/06

Box3

A B C D E F G H I
pUC19pKD46-1pKD13pKD46-2RP4 (Amp,Kan,Tet)R35:tetR2:1 RBS:tetR2:1 RBS:cIRP1 x K1200 Kan
RP1 xK1200 NDRP4 (Amp,Kan,Tet)Top10RP1 xK1200 Ampp0X38 Kan (F)PCVtop10Frost FR0040
B0015E0430 pSB1A2I05115spo0A topo 1:1spo0A topo 1:2Terminator BBa_B0015spoIIE topo 1:12:2 RBS:cIi51840
E0030 RBS strongR0040 pSB1A2I0500spoIIE topo 1:2E0840C0051 cI reppSpoIIa + YFPI12007 pSB2K3pKD46 in DH10B
pKD46 x RlambdapKD46 x pOX38 pBca1020-Bca1022 lib hit C2 pBca1020-Bca1022 lib hit D7 pBca1020-Bca1022 lib hit C5 pBca1020-Bca1022 lib hit H12 pBca1020-Bca1022 lib hit E1 pBca1020-Bca1022 lib hit B10 pBca1020-Bca1022 lib hit E11
pBca1020-Bca1022 lib hit F3 pBca1020-Bca1022 lib hit A5 pBca1020-Bca1022 lib hit F6 pBca1020-Bca1022 lib hit E10 pBca1020-Bca1022 lib hit C1 pBca1020-Bca1022 lib hit B9 pBca1020-Bca1022 lib hit F4 pBca1020-Bca1022 lib hit D7 pBca1020-Bca1022 lib hit C2
pBca1020-Bca1022 lib hit F12 pBca1020-Bca1022 lib hit A2 pBca1020-Bca1022 lib hit E8 1022A 1022B 1022C 1022D 1022E

Box4: TlambdaOlambda F plasmid KCM competent cells.

Box5: Rlambda R plasmid KCM competent cells.

Box6: Glycerol Stocks of EC100D::pir+ and pOX38

Box7: pSB3C6-J01122 KCM Competent cells (orange and green stripes).

Box 8: TG1

Back Compartment: Olambda F plasmid (OriT knockout) KCM competent cells.

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