Berk2006-StockBoxes
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Procedure: Combine equal volumes of cells and glycerol (50% cells, 50%glycerol). Freeze in cryo vial.
Box1
| A | B | C | D | E | F | G | H | I | |
|---|---|---|---|---|---|---|---|---|---|
| J01004 | J01008 | J01063 | J01028 | J01064 | J01021 | J01022 | J01023 | J01024(KAN | |
| J01020 | J01030 | J01099 | J01100 | J01027 | J01052 | J01055 | J01050 | J01060 | |
| J01057 | J01058 | J01059 | J01067 | J01068 | J01076 | J01076 | J01075 | J01093 | |
| J01092 | J01094 | J01096 | J01098 | J01129/MC1061 | J01002/MC1061 | J01093/MC1061 | pSB3C6-J01122/TGI | pAC997-1/MC1061 | |
| J01001 | J01002 | J01003 | J01109 | J01021 | J01022 | J01023 | J01124 | J01126 | |
| J01127 | J01066 | pSB3C6-J01022-B | BW20767 | - | pSB3C6-J01093 (TGI/Clone 1) | pSB3C6-J01024 (TGI/Clone 1) | TG1 | ||
| pJ23006-I13522 | pJ23006-J23007 | pJ23006-J23008 | pSB1A2-J23012 | pSB1A2-J23018-2 | "pSB1A2"-J01007 | "pSB1A2"-J01006 | |||
Box2
| A | B | C | D | E | F | G | H | I | ||
|---|---|---|---|---|---|---|---|---|---|---|
| LOCK2 | ID500 | IL2007 | R0040 | R0051 | ROO80 pSB2K3 | PK3 | PK2 | E0420 pSB1A2 | ||
| E0430 | E0840 | 13521 SB1A3 | I3006 | E0024 | E1010 pSB2K3 | C0051 | C0040 tetR | B0015 | ||
| BBa_B0020 RBS strong | B0034 | E6030 pSB1Ak3 | oriTF | 0lambda | oriTR | oriTR | lock1 | traJR | ||
| oriTF on RFP | J01020 | J01021 | TlambdaOlambda | Rlambda | T6 | J01024 | J01022 | J01023 | ||
| pSpoIIE Amp | pSpoIIE kan | pSB1A2-I13522 in JM109 | pSB1A2-S03155 | 994 Library 06/27/06 | pSB1A2-T9002 in TGI | pSB1A2-P0412 in TGI | pSB1A2-I13603 in TGI | pSB1A2-F2620 in TGI | ||
| pSB1A2-I13601 in TGI | pSB1AK3-F1610 in TGI | pSB1A2-R0040+F1610#1_07/25/06 | pSB1A2-F2620+P0412#1_07/25/06 | |||||||
Box3
| A | B | C | D | E | F | G | H | I | |
|---|---|---|---|---|---|---|---|---|---|
| pUC19 | pKD46-1 | pKD13 | pKD46-2 | RP4 (Amp,Kan,Tet) | R35:tetR | 2:1 RBS:tetR | 2:1 RBS:cI | RP1 x K1200 Kan | |
| RP1 xK1200 ND | RP4 (Amp,Kan,Tet) | Top10 | RP1 xK1200 Amp | p0X38 Kan (F) | PCV | top10 | Frost F | R0040 | |
| B0015 | E0430 pSB1A2 | I05115 | spo0A topo 1:1 | spo0A topo 1:2 | Terminator BBa_B0015 | spoIIE topo 1:1 | 2:2 RBS:cI | i51840 | |
| E0030 RBS strong | R0040 pSB1A2 | I0500 | spoIIE topo 1:2 | E0840 | C0051 cI rep | pSpoIIa + YFP | I12007 pSB2K3 | pKD46 in DH10B | |
| pKD46 x Rlambda | pKD46 x pOX38 | pBca1020-Bca1022 lib hit C2 | pBca1020-Bca1022 lib hit D7 | pBca1020-Bca1022 lib hit C5 | pBca1020-Bca1022 lib hit H12 | pBca1020-Bca1022 lib hit E1 | pBca1020-Bca1022 lib hit B10 | pBca1020-Bca1022 lib hit E11 | |
| pBca1020-Bca1022 lib hit F3 | pBca1020-Bca1022 lib hit A5 | pBca1020-Bca1022 lib hit F6 | pBca1020-Bca1022 lib hit E10 | pBca1020-Bca1022 lib hit C1 | pBca1020-Bca1022 lib hit B9 | pBca1020-Bca1022 lib hit F4 | pBca1020-Bca1022 lib hit D7 | pBca1020-Bca1022 lib hit C2 | |
| pBca1020-Bca1022 lib hit F12 | pBca1020-Bca1022 lib hit A2 | pBca1020-Bca1022 lib hit E8 | 1022A | 1022B | 1022C | 1022D | 1022E | ||
Box4: TlambdaOlambda F plasmid KCM competent cells.
Box5: Rlambda R plasmid KCM competent cells.
Box6: Glycerol Stocks of EC100D::pir+ and pOX38
Box7: pSB3C6-J01122 KCM Competent cells (orange and green stripes).
Box 8: TG1
Back Compartment: Olambda F plasmid (OriT knockout) KCM competent cells.
