Berk2010-Christoph: Difference between revisions
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Gel purified Bjh1882 and Bjh2245. <br> | Gel purified Bjh1882 and Bjh2245. <br> | ||
Small fragment zymo cleanup of BCA1091. <br> | Small fragment zymo cleanup of BCA1091. <br> | ||
==[[User:Christoph Neyer|Christoph Neyer]] 16:35, 7 June 2010 (EDT)== | |||
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps. | |||
Re-cutting Bjh2245 and Bjh1882. | |||
Small fragment digest of Bjh2245. | |||
Gel purified Bjh1882. | |||
Revision as of 13:35, 7 June 2010
==Christoph Neyer 15:54, 7 June 2010 (EDT)==
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps. Re-cutting Bjh2245 and Bjh1882. Small fragment digest of Bjh2245. Gel purified Bjh1882.
