Berk2010-Christoph: Difference between revisions
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Small fragment zymo cleanup of BCA1091. <br> | Small fragment zymo cleanup of BCA1091. <br> | ||
==[[User:Christoph Neyer|Christoph Neyer]] 16:35, 7 June 2010 (EDT)== | ==[[User:Christoph Neyer|Christoph Neyer]] 16:35, 7 June 2010 (EDT)== | ||
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps. | Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps. <br> | ||
Re-cutting Bjh2245 and Bjh1882. | Re-cutting Bjh2245 and Bjh1882. <br> | ||
Small fragment digest of Bjh2245. | Small fragment digest of Bjh2245. <br> | ||
Gel purified Bjh1882. | Gel purified Bjh1882. <br> | ||
Revision as of 13:36, 7 June 2010
==Christoph Neyer 15:54, 7 June 2010 (EDT)==
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.
