Berk2010-Christoph: Difference between revisions
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==[[User:Christoph Neyer|Christoph Neyer]] 15:54, 7 June 2010 (EDT)== | ==[[User:Christoph Neyer|Christoph Neyer]] 15:54, 7 June 2010 (EDT)== | ||
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet). <br> | Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet). <br> | ||
Gel purified Bjh1882 and Bjh2245. <br> | Gel purified Bjh1882 and Bjh2245. <br> |
Revision as of 14:59, 7 June 2010
Christoph Neyer 15:54, 7 June 2010 (EDT)
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.