Berk2010-Christoph: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 9: | Line 9: | ||
[[Image:Gel_purify_bjh1882.JPG|300px]]<br> | [[Image:Gel_purify_bjh1882.JPG|300px]]<br> | ||
Gel purified Bjh1882. <br> | Gel purified Bjh1882. <br> | ||
==[[User:Christoph Neyer|Christoph Neyer]] 19:30, 7 June 2010 (EDT)== | |||
Bjh1882 Vectors: AC | |||
Bjh2245 Vectors: KA | |||
Bca1091 Vectors: CK | |||
Revision as of 16:30, 7 June 2010
Christoph Neyer 15:54, 7 June 2010 (EDT)
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.
Christoph Neyer 19:30, 7 June 2010 (EDT)
Bjh1882 Vectors: AC Bjh2245 Vectors: KA Bca1091 Vectors: CK
