Berk2010-Christoph: Difference between revisions
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Bjh2245 Vectors: KA <br> | Bjh2245 Vectors: KA <br> | ||
Bca1091 Vectors: CK <br> | Bca1091 Vectors: CK <br> | ||
pMLL4-AC <br> | |||
pMLL5-CK <br> | |||
pMLL6-KA <br> | |||
pMLL7-AK <br> | |||
pMLL8-KC <br> | |||
pMLL9-CA <br> | |||
Bjh1882+pMLL4 <br> | |||
Bjh2245+pMLL6 <br> | |||
Bca1091+pMLL5 <br> | |||
Revision as of 16:42, 7 June 2010
Christoph Neyer 15:54, 7 June 2010 (EDT)
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet).
Gel purified Bjh1882 and Bjh2245.
Small fragment zymo cleanup of BCA1091.
Christoph Neyer 16:35, 7 June 2010 (EDT)
Couldn't see fragments. Turns out Bjh2245 is 97bp and Bjh1882 is ~680bps.
Re-cutting Bjh2245 and Bjh1882.
Small fragment digest of Bjh2245.
Gel purified Bjh1882.
Christoph Neyer 19:30, 7 June 2010 (EDT)
Bjh1882 Vectors: AC
Bjh2245 Vectors: KA
Bca1091 Vectors: CK
pMLL4-AC
pMLL5-CK
pMLL6-KA
pMLL7-AK
pMLL8-KC
pMLL9-CA
Bjh1882+pMLL4
Bjh2245+pMLL6
Bca1091+pMLL5
