Berk2010-Conor

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Conor McClune 14:38, 7 June 2010 (EDT)

(with Tahoura)
Transformed (spec)1256-2343 and (spec)1256-2344 into MG1655. Used minipreps of spec1256-2343 and spec1256-2344 from box and diluted 10x. Mixed 50ul MG1655 with 17.5ul KCN and added 1ul diluted DNA. Incubated for 1hr and plated on Spec. Left in incubator to grow
Plated pB6 (BAC), place in incubator. Needs to be picked and miniprepped tomorrow.
Transformed pBca1256-Bjh1934 into MG1655. Used procedure from (1)
Attempted to enter parts into clotho --> would not save

Manual Assembly

To Do:

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