Berk2010-Tahoura: Difference between revisions
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[[June]] | [[June]] | ||
=[[User:Tahoura Samad|Tahoura Samad]] 18:16, 6 July 2010 (EDT)= | |||
==PDD Subgroup Work== | |||
*talked to Jin. He was not able to look at the combinations from Friday. :( | |||
*Jin says to let all the different combinations rest, and focus on the 1934 payload. | |||
*Jin also says to start the minimal media testing to see if pb6 works. | |||
==Lifeact== | |||
*ran colony PCR for 32 colonies. | |||
==NLS== | |||
*check sequencing results with Amy. | |||
=[[User:Tahoura Samad|Tahoura Samad]] 13:22, 5 July 2010 (EDT)= | |||
*picked and grew up 1, 2, 6, 7, to make competent cells. | |||
=[[User:Tahoura Samad|Tahoura Samad]] 13:22, 2 July 2010 (EDT)= | =[[User:Tahoura Samad|Tahoura Samad]] 13:22, 2 July 2010 (EDT)= | ||
*most of my transformations with the new competent cells were successful. | *most of my transformations with the new competent cells were successful. |
Revision as of 15:16, 6 July 2010
Tahoura Samad 18:16, 6 July 2010 (EDT)
PDD Subgroup Work
- talked to Jin. He was not able to look at the combinations from Friday. :(
- Jin says to let all the different combinations rest, and focus on the 1934 payload.
- Jin also says to start the minimal media testing to see if pb6 works.
Lifeact
- ran colony PCR for 32 colonies.
NLS
- check sequencing results with Amy.
Tahoura Samad 13:22, 5 July 2010 (EDT)
- picked and grew up 1, 2, 6, 7, to make competent cells.
Tahoura Samad 13:22, 2 July 2010 (EDT)
- most of my transformations with the new competent cells were successful.
Competent cell | Number | PDD | Growth in initial competent cells | Growth in Small Scale Competent Cells | Result of Assay with Jin | Conclusion | |
pBca156-Bjh2343 | 1 | 2331 | 0 | tons of colonies | NOT DONE | transform new, assay | |
pBca156-Bjh2343 | 1 | 2333 | 0 | tons of colonies | NOT DONE | transform new, assay | |
pBca156-Bjh2343 | 1 | 2348 | 1 colony (twice) | tons of colonies | NOT DONE | transform new, assay | |
pBca156-Bjh2343 | 1 | 2349 | 0 | 50-100 colonies | NOT DONE | transform new, assay | |
pBca156-Bjh2343 | 1 | 2291 | 0 | 100-200 colonies | NOT DONE | transform new, assay | |
pBca156-Bjh2343 with BAC | 7 | 2331 | 0 | 15 colonies | NOT DONE | Today | |
pBca156-Bjh2343 with BAC | 7 | 2333 | 0 | 2 colonies | NOT DONE | Today | |
pBca156-Bjh2343 with BAC | 7 | 2348 | 0 | 4 colonies | NOT DONE | Today | |
pBca156-Bjh2343 with BAC | 7 | 2349 | 0 | 0 | DEAD | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh2343 with BAC | 7 | 2291 | 0 | 0 | DEAD | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh2344 | 2 | 2331 | 0 | tons of colonies | NOT DONE | make new, transform, assay | |
pBca156-Bjh2344 | 2 | 2333 | 0 | tons of colonies | NOT DONE | make new, transform, assay | |
pBca156-Bjh2344 | 2 | 2348 | 12 colonies | TO DO | V (UNSTABLE) | make new, transform new and old, assay | |
pBca156-Bjh2344 | 2 | 2349 | 6 colonies | TO DO | V V | make new, transform new and old, assay | |
pBca156-Bjh2344 | 2 | 2291 | 7 colonies | TO DO | V V | make new, transform new and old, assay | |
pBca156-Bjh2344 with BAC | 6 | 2331 | tons of colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh2344 with BAC | 6 | 2333 | tons of colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh2344 with BAC | 6 | 2348 | 1 colony (twice) | 100-200 colonies | NOT DONE | Today | |
pBca156-Bjh2344 with BAC | 6 | 2349 | 1 colony(twice) | 35-50 colonies | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh2344 with BAC | 6 | 2291 | 35-50 colonies | 100-200 colonies | V V V | transform new, assay | |
pBca156-Bjh1934 | 3 | 2331 | 35-50 colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh1934 | 3 | 2333 | <100 colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh1934 | 3 | 2348 | tons of colonies | X | V V | transform old, assay | |
pBca156-Bjh1934 | 3 | 2349 | tons of colonies | X | V V | transform old, assay | |
pBca156-Bjh1934 | 3 | 2291 | tons of colonies | X | V | transform old,assay | |
pBca156-Bjh1934 with BAC | 5 | 2331 | tons of colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh1934 with BAC | 5 | 2333 | tons of colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh1934 with BAC | 5 | 2348 | tons of colones | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh1934 with BAC | 5 | 2349 | 1o colonies | X | X | \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ | |
pBca156-Bjh1934 with BAC | 5 | 2291 | tons of colonies | X | V V V | transform old, assay |
- competent cell checks for new 1 and new 6 looked good.
- miniprepped IBB as CA lefty, CK lefty and CK righty, and sent out for sequencing with ca998.
Tahoura Samad 13:38, 1 July 2010 (EDT)
- controls for JTK030 looked good with no growth on Kan, Amp, Cam, good growth on LB, and a little growth on Spec, which is the normal result because of mutations in the bacteria. Those are labeled with double black stripes, in a box in the back of the -80 in the 2nd row from the top.
- picked and grew up the results of PCA for the new nuclear localization tag. They had plenty of colonies, with only one background green colony. Will miniprep them tomorrow.
- did small scale competent cell prep of 1 and 6.
- did the following transformations (1old:2348), (5 old:2291) (6 old:2291) (6 new:2291),(3 old :2348) (3 old: 2349) (3 old:2291) (2 old:2348) (2 old: 2349)(2 old:2291) (2 new:2348) (2 new:2349) (2 new: 2291), (1 new:2331) (1 new:2333) (1 new:2348) (1 new:2349) (1 new: 2291) (6 new 2348) (6 new:2349), (IBB into lefty).
- redid assembly of stage 0 of life act but with 2.4 μL of each DNA and 1.2 μL of 5x NEB2.