Berk2010-Tahoura: Difference between revisions
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===[[User:Tahoura Samad|Tahoura Samad]] 14:15, 7 June 2010 (EDT)=== | ===[[User:Tahoura Samad|Tahoura Samad]] 14:15, 7 June 2010 (EDT)=== | ||
referenced Jins notes:[[Image:jin_Notes2.JPG|200px]] | |||
1. (with Conor) Transformed Mg1655 cells (from -80 freezer) from Jin with two PDD 2343, 2344( from freezer box). | 1. (with Conor) Transformed Mg1655 cells (from -80 freezer) from Jin with two PDD 2343, 2344( from freezer box). | ||
-Diluted DNA by 10x ( 9 μL dH20, 1 DNA), added 1 μL to cells. | -Diluted DNA by 10x ( 9 μL dH20, 1 DNA), added 1 μL to cells. | ||
2. pB6 (BAC) see image, arrived from Germany. Wear gloves because its BSL 2. Currently in fridge. TIm streaked it on a plate. (Note: Streak such that the concentration is reduced as you go along.) The part we need arrived in bacteria, so we need to grow them up and miniprep them to get the DNA we need. | |||
3. Transformed MG1655 cells with pBca1256-Bjh1934 (from Jin's Overflow box 1). Tube name: 1256-1934 into MG1655 | |||
4. Plated our transformations (2343 in MG1655 and 2344 in 1655) on Spec plates (yellow). Plate names: 2343 into MG1655, 2344 into MG1655 | |||
Revision as of 13:10, 7 June 2010
Tahoura Samad 14:15, 7 June 2010 (EDT)
referenced Jins notes:
1. (with Conor) Transformed Mg1655 cells (from -80 freezer) from Jin with two PDD 2343, 2344( from freezer box). -Diluted DNA by 10x ( 9 μL dH20, 1 DNA), added 1 μL to cells. 2. pB6 (BAC) see image, arrived from Germany. Wear gloves because its BSL 2. Currently in fridge. TIm streaked it on a plate. (Note: Streak such that the concentration is reduced as you go along.) The part we need arrived in bacteria, so we need to grow them up and miniprep them to get the DNA we need. 3. Transformed MG1655 cells with pBca1256-Bjh1934 (from Jin's Overflow box 1). Tube name: 1256-1934 into MG1655 4. Plated our transformations (2343 in MG1655 and 2344 in 1655) on Spec plates (yellow). Plate names: 2343 into MG1655, 2344 into MG1655
