Berk2010-Tim

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Tim Hsiau (Talk | contribs)
(New page: Image:Jin_Notes.JPG first column is PDD: payload delivery device second column is the payload plus some stuff that may inhibit vacuole merging (secretion system) third column is the...)
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Revision as of 19:06, 4 June 2010

Image:Jin_Notes.JPG

first column is PDD: payload delivery device

second column is the payload plus some stuff that may inhibit vacuole merging (secretion system)

third column is the BAC that contains other secretion system components

PDD's are not stable, need to make competent cells of BAC plus payload and do fresh transforms of PDD's everytime you assay


Other notes:

Mg needs to be added for all steps of cloning the PDD's
1M MgSO4
10µL into rescue step (after heatshock)
400µL spread on plate (if +Spec, add 20µL and spread)
30µL into ea mL TB media (use TB for growing up for assay)
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