Berk2010-Tim
From OpenWetWare
first column is PDD: payload delivery device
second column is the payload plus some stuff that may inhibit vacuole merging (secretion system)
third column is the BAC that contains other secretion system components
PDD's are not stable, need to make competent cells of BAC plus payload and do fresh transforms of PDD's everytime you assay
We have to work with MG1655
Other notes:
Mg needs to be added for all steps of cloning the PDD's 1M MgSO4 10µL into rescue step (after heatshock) 400µL spread on plate (if +Spec, add 20µL and spread) 30µL into ea mL TB media (use TB for growing up for assay)