Berkeley iGEM10

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(New page: ==~~~~== EcoXho's for the first round of assembly (ecobammed into assembly vectors) 400px appropriate fragment lengths <pre> layout on ecoXho gel FRAGMENTS ...)
(Tim Hsiau 16:16, 1 June 2010 (EDT))
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<pre>
<pre>
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layout on ecoXho gel FRAGMENTS
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layout on ecoXho gel FRAGMENTS part size
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1 6-2 1307 1605
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1 6-2 1307 1605 35
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2 6-11 1318 1605
+
2 6-11 1318 1605 46
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3 6-2 1307 1605
+
3 6-2 1307 1605 35
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4 6-11 1318 1605
+
4 6-11 1318 1605 46
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5 6-4 2703 1605
+
5 6-4 2703 1605 1431
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6 6-12 1869 1605
+
6 6-12 1869 1605 597
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7 6-4 2703 1605
+
7 6-4 2703 1605 1431
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8 6-12 1869 1605
+
8 6-12 1869 1605 597
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9 6-5 1513 1605
+
9 6-5 1513 1605 241
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10 6-13 1671 1605
+
10 6-13 1671 1605 399
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11 6-5 1513 1605
+
11 6-5 1513 1605 241
-
12 6-13 1671 1605
+
12 6-13 1671 1605 399
-
13 6-7 3060 1605
+
13 6-7 3060 1605 1788
-
14 6-19 1500 1605
+
14 6-19 1500 1605 228
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15 6-7 3060 1605
+
15 6-7 3060 1605 1788
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16 6-19 1500 1605
+
16 6-19 1500 1605 228
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17 6-22 2298 1605
+
17 6-22 2298 1605 1026
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18 7-9 1797 1680
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18 7-9 1797 1680 597
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19 6-22 2298 1605
+
19 6-22 2298 1605 1026
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20 7-10 1300 1680
+
20 7-10 1300 1680 100
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21 6-23 1363 1605
+
21 6-23 1363 1605 91
-
22 7-11 1246 1680
+
22 7-11 1246 1680 46
-
23 6-23 1363 1605
+
23 6-23 1363 1605 91
-
24 7-13 1599 1680
+
24 7-13 1599 1680 399
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25 6-24 #N/A 1605
+
25 6-24 #N/A 1605 #N/A
-
26 7-14 1599 1680
+
26 7-14 1599 1680 399
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27 6-24 #N/A 1605
+
27 6-24 #N/A 1605 #N/A
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28 7-19 1428 1680
+
28 7-19 1428 1680 228
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29 7-2 1235 1680
+
29 7-2 1235 1680 35
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30 7-23 1291 1680
+
30 7-23 1291 1680 91
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31 7-5 1441 1680
+
31 7-5 1441 1680 241
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32 7-24 #N/A 1680
+
32 7-24 #N/A 1680 #N/A
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33 7-24 #N/A 1680
+
33 7-24 #N/A 1680 #N/A
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34 5-23 1291 1473
+
34 5-23 1291 1473 91
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35 5-3 1233 1473
+
35 5-3 1233 1473 33
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36 5-23 1291 1473
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36 5-23 1291 1473 91
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37 5-3 1233 1473
+
37 5-3 1233 1473 33
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38 8-1 1307 1473
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38 8-1 1307 1473 35
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39 5-8 2444 1473
+
39 5-8 2444 1473 1244
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40 8-1 1307 1473
+
40 8-1 1307 1473 35
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41 5-8 2444 1473
+
41 5-8 2444 1473 1244
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42 8-4 2703 1473
+
42 8-4 2703 1473 1431
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43 5-15 1290 1473
+
43 5-15 1290 1473 90
-
44 8-4 2703 1473
+
44 8-4 2703 1473 1431
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45 5-20 2023 1473
+
45 5-20 2023 1473 823
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46 8-6 1591 1473
+
46 8-6 1591 1473 319
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47 5-20 2023 1473
+
47 5-20 2023 1473 823
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48 8-6 1591 1473
+
48 8-6 1591 1473 319
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+
-
+
-
+
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1 8-17 3117 1473
+
1 8-17 3117 1473 1845
-
2 4-10 1166 1679
+
2 4-10 1166 1679 100
-
3 8-17 3117 1473
+
3 8-17 3117 1473 1845
-
4 4-10 1166 1679
+
4 4-10 1166 1679 100
-
5 8-18 3090 1473
+
5 8-18 3090 1473 1818
-
6 4-16 1156 1679
+
6 4-16 1156 1679 90
-
7 8-18 3090 1473
+
7 8-18 3090 1473 1818
-
8 4-16 1156 1679
+
8 4-16 1156 1679 90
-
9 8-21 1504 1473
+
9 8-21 1504 1473 232
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10 4-17 2911 1679
+
10 4-17 2911 1679 1845
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11 8-21 1504 1473
+
11 8-21 1504 1473 232
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12 4-17 2911 1679
+
12 4-17 2911 1679 1845
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13 4-9 1663 1679
+
13 4-9 1663 1679 597
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14 4-18 2884 1679
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14 4-18 2884 1679 1818
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15 4-9 1663 1679
+
15 4-9 1663 1679 597
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16 4-18 2884 1679
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16 4-18 2884 1679 1818
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17 9-3 1099 1605
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17 9-3 1099 1605 33
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18 9-20 1889 1605
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18 9-20 1889 1605 823
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19 9-3 1099 1605
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19 9-3 1099 1605 33
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20 9-20 1889 1605
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20 9-20 1889 1605 823
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21 9-8 2310 1605
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21 9-8 2310 1605 1244
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22 9-23 1157 1605
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22 9-23 1157 1605 91
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23 9-8 2310 1605
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23 9-8 2310 1605 1244
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24 9-23 1157 1605
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24 9-23 1157 1605 91
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25 9-11 1112 1605
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25 9-11 1112 1605 46
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26
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26
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27 9-11 1112 1605
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27 9-11 1112 1605 46
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28
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28
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29 9-13 1465 1605
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29 9-13 1465 1605 399
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30
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30
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31 9-13 1465 1605
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31 9-13 1465 1605 399
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</pre>
</pre>

Revision as of 19:59, 1 June 2010

Tim Hsiau 16:16, 1 June 2010 (EDT)

EcoXho's for the first round of assembly (ecobammed into assembly vectors)


appropriate fragment lengths


layout on ecoXho gel		FRAGMENTS		part size
1	6-2	1307	1605	35
2	6-11	1318	1605	46
3	6-2	1307	1605	35
4	6-11	1318	1605	46
5	6-4	2703	1605	1431
6	6-12	1869	1605	597
7	6-4	2703	1605	1431
8	6-12	1869	1605	597
9	6-5	1513	1605	241
10	6-13	1671	1605	399
11	6-5	1513	1605	241
12	6-13	1671	1605	399
13	6-7	3060	1605	1788
14	6-19	1500	1605	228
15	6-7	3060	1605	1788
16	6-19	1500	1605	228
17	6-22	2298	1605	1026
18	7-9	1797	1680	597
19	6-22	2298	1605	1026
20	7-10	1300	1680	100
21	6-23	1363	1605	91
22	7-11	1246	1680	46
23	6-23	1363	1605	91
24	7-13	1599	1680	399
25	6-24	#N/A	1605	#N/A
26	7-14	1599	1680	399
27	6-24	#N/A	1605	#N/A
28	7-19	1428	1680	228
29	7-2	1235	1680	35
30	7-23	1291	1680	91
31	7-5	1441	1680	241
32	7-24	#N/A	1680	#N/A
33	7-24	#N/A	1680	#N/A
34	5-23	1291	1473	91
35	5-3	1233	1473	33
36	5-23	1291	1473	91
37	5-3	1233	1473	33
38	8-1	1307	1473	35
39	5-8	2444	1473	1244
40	8-1	1307	1473	35
41	5-8	2444	1473	1244
42	8-4	2703	1473	1431
43	5-15	1290	1473	90
44	8-4	2703	1473	1431
45	5-20	2023	1473	823
46	8-6	1591	1473	319
47	5-20	2023	1473	823
48	8-6	1591	1473	319
				
				
				
1	8-17	3117	1473	1845
2	4-10	1166	1679	100
3	8-17	3117	1473	1845
4	4-10	1166	1679	100
5	8-18	3090	1473	1818
6	4-16	1156	1679	90
7	8-18	3090	1473	1818
8	4-16	1156	1679	90
9	8-21	1504	1473	232
10	4-17	2911	1679	1845
11	8-21	1504	1473	232
12	4-17	2911	1679	1845
13	4-9	1663	1679	597
14	4-18	2884	1679	1818
15	4-9	1663	1679	597
16	4-18	2884	1679	1818
17	9-3	1099	1605	33
18	9-20	1889	1605	823
19	9-3	1099	1605	33
20	9-20	1889	1605	823
21	9-8	2310	1605	1244
22	9-23	1157	1605	91
23	9-8	2310	1605	1244
24	9-23	1157	1605	91
25	9-11	1112	1605	46
26				
27	9-11	1112	1605	46
28				
29	9-13	1465	1605	399
30				
31	9-13	1465	1605	399
32				
33				
34				
35				

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