Berkeley iGEM10

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(Tim Hsiau 16:16, 1 June 2010 (EDT))
(Tim Hsiau 16:16, 1 June 2010 (EDT))
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EcoXho's for the first round of assembly (ecobammed into assembly vectors)
EcoXho's for the first round of assembly (ecobammed into assembly vectors)
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[[Image:Igem_ecoxhos.png|400px]]
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[[Image:Igem ecoxhos annotated.png|400px]]

Revision as of 20:18, 1 June 2010

Tim Hsiau 16:16, 1 June 2010 (EDT)

EcoXho's for the first round of assembly (ecobammed into assembly vectors)


appropriate fragment lengths


layout on ecoXho gel		FRAGMENTS		part size
1	6-2	1307	1605	35
2	6-11	1318	1605	46
3	6-2	1307	1605	35
4	6-11	1318	1605	46
5	6-4	2703	1605	1431
6	6-12	1869	1605	597
7	6-4	2703	1605	1431
8	6-12	1869	1605	597
9	6-5	1513	1605	241
10	6-13	1671	1605	399
11	6-5	1513	1605	241
12	6-13	1671	1605	399
13	6-7	3060	1605	1788
14	6-19	1500	1605	228
15	6-7	3060	1605	1788
16	6-19	1500	1605	228
17	6-22	2298	1605	1026
18	7-9	1797	1680	597
19	6-22	2298	1605	1026
20	7-10	1300	1680	100
21	6-23	1363	1605	91
22	7-11	1246	1680	46
23	6-23	1363	1605	91
24	7-13	1599	1680	399
25	6-24	#N/A	1605	#N/A
26	7-14	1599	1680	399
27	6-24	#N/A	1605	#N/A
28	7-19	1428	1680	228
29	7-2	1235	1680	35
30	7-23	1291	1680	91
31	7-5	1441	1680	241
32	7-24	#N/A	1680	#N/A
33	7-24	#N/A	1680	#N/A
34	5-23	1291	1473	91
35	5-3	1233	1473	33
36	5-23	1291	1473	91
37	5-3	1233	1473	33
38	8-1	1307	1473	35
39	5-8	2444	1473	1244
40	8-1	1307	1473	35
41	5-8	2444	1473	1244
42	8-4	2703	1473	1431
43	5-15	1290	1473	90
44	8-4	2703	1473	1431
45	5-20	2023	1473	823
46	8-6	1591	1473	319
47	5-20	2023	1473	823
48	8-6	1591	1473	319
				
				
				
1	8-17	3117	1473	1845
2	4-10	1166	1679	100
3	8-17	3117	1473	1845
4	4-10	1166	1679	100
5	8-18	3090	1473	1818
6	4-16	1156	1679	90
7	8-18	3090	1473	1818
8	4-16	1156	1679	90
9	8-21	1504	1473	232
10	4-17	2911	1679	1845
11	8-21	1504	1473	232
12	4-17	2911	1679	1845
13	4-9	1663	1679	597
14	4-18	2884	1679	1818
15	4-9	1663	1679	597
16	4-18	2884	1679	1818
17	9-3	1099	1605	33
18	9-20	1889	1605	823
19	9-3	1099	1605	33
20	9-20	1889	1605	823
21	9-8	2310	1605	1244
22	9-23	1157	1605	91
23	9-8	2310	1605	1244
24	9-23	1157	1605	91
25	9-11	1112	1605	46
26				
27	9-11	1112	1605	46
28				
29	9-13	1465	1605	399
30				
31	9-13	1465	1605	399
32				
33				
34				
35				

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