Beta-galactosidase - Revision history

Jakob Suckale: links to protocols and X-Gal

Jakob Suckale — Tue, 10 Jun 2008 15:28:16 GMT

links to protocols and X-Gal

←Older revision		Revision as of 15:28, 10 June 2008
Line 15:		Line 15:

	==Protocols==		==Protocols==
-	~~See~~ [[beta-galactosidase assay]]	+	* [[beta-galactosidase assay]]
		+	* [[LacZ staining of cells]]
		+
		+	==See also==
		+	* [[X-Gal]]

	==Reference==		==Reference==
Line 31:		Line 35:
	#Vidal-Aroca-2006 pmid=16629389		#Vidal-Aroca-2006 pmid=16629389
	</biblio>		</biblio>
		+

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Austin J. Che at 20:48, 4 April 2008

Austin J. Che — Fri, 04 Apr 2008 20:48:05 GMT

←Older revision		Revision as of 20:48, 4 April 2008
Line 15:		Line 15:

	==Protocols==		==Protocols==
-	#[[~~Beta-Galactosidase Assay (A better Miller)]]~~	+	See [[beta-galactosidase assay]]
-	~~#[[BE.109:Protein engineering/Assessing~~ beta-galactosidase]]	+
-	~~#See also [http://www.fccc.edu/research/labs/golemis/betagal/beta_gal_yeast.html Beta-galactosidase assays in yeast~~]	+

	==Reference==		==Reference==

Austin J. Che at 18:33, 20 March 2008

Austin J. Che — Thu, 20 Mar 2008 18:33:58 GMT

←Older revision		Revision as of 18:33, 20 March 2008
Line 11:		Line 11:
	*You can measure lacZ activity using flow cytometry. See [http://www.cyto.purdue.edu/flowcyt/research/micrflow/jepras/jepras2.htm A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion]		*You can measure lacZ activity using flow cytometry. See [http://www.cyto.purdue.edu/flowcyt/research/micrflow/jepras/jepras2.htm A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion]
	**This paper uses [http://probes.invitrogen.com/media/pis/mp02904.pdf C<sub>12</sub>FDG] from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like ''Escherichia coli'' <cite>Plovins-ApplEnvironMicrobio-1994</cite>		**This paper uses [http://probes.invitrogen.com/media/pis/mp02904.pdf C<sub>12</sub>FDG] from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like ''Escherichia coli'' <cite>Plovins-ApplEnvironMicrobio-1994</cite>
		+	* Another fluorogenic substrate is 4-methylumbelliferyl β-D-galactopyranoside (MUG) which works in bacteria, yeast, and mammalian cells (without requiring permeabilization/lysis). <cite>Vidal-Aroca-2006</cite>
	*Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype <cite>Zamenhof-JBacteriol-1972</cite>. '''(If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.)'''		*Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype <cite>Zamenhof-JBacteriol-1972</cite>. '''(If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.)'''

Line 30:		Line 31:
	#WorthingtonBiochem http://www.worthington-biochem.com/BG/default.html		#WorthingtonBiochem http://www.worthington-biochem.com/BG/default.html
	#Serebriiskii-AnalBiochem-2000 pmid=10998258		#Serebriiskii-AnalBiochem-2000 pmid=10998258
		+	#Vidal-Aroca-2006 pmid=16629389
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Reshma P. Shetty at 20:38, 9 January 2008

Reshma P. Shetty — Wed, 09 Jan 2008 20:38:29 GMT

←Older revision		Revision as of 20:38, 9 January 2008
Line 16:		Line 16:
	#[[Beta-Galactosidase Assay (A better Miller)]]		#[[Beta-Galactosidase Assay (A better Miller)]]
	#[[BE.109:Protein engineering/Assessing beta-galactosidase]]		#[[BE.109:Protein engineering/Assessing beta-galactosidase]]
		+	#See also [http://www.fccc.edu/research/labs/golemis/betagal/beta_gal_yeast.html Beta-galactosidase assays in yeast]

	==Reference==		==Reference==
Line 28:		Line 29:
	#Thibodeau-Biotechniques-2004 pmid=15038156		#Thibodeau-Biotechniques-2004 pmid=15038156
	#WorthingtonBiochem http://www.worthington-biochem.com/BG/default.html		#WorthingtonBiochem http://www.worthington-biochem.com/BG/default.html
		+	#Serebriiskii-AnalBiochem-2000 pmid=10998258
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Reshma P. Shetty at 22:02, 8 November 2007

Reshma P. Shetty — Thu, 08 Nov 2007 22:02:26 GMT

←Older revision		Revision as of 22:02, 8 November 2007
Line 27:		Line 27:
	#Zamenhof-JBacteriol-1972 pmid=4552986		#Zamenhof-JBacteriol-1972 pmid=4552986
	#Thibodeau-Biotechniques-2004 pmid=15038156		#Thibodeau-Biotechniques-2004 pmid=15038156
		+	#WorthingtonBiochem http://www.worthington-biochem.com/BG/default.html
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Reshma P. Shetty at 19:23, 3 November 2007

Reshma P. Shetty — Sat, 03 Nov 2007 19:23:55 GMT

←Older revision		Revision as of 19:23, 3 November 2007
Line 26:		Line 26:
	// original β-galactosidase assay by Miller		// original β-galactosidase assay by Miller
	#Zamenhof-JBacteriol-1972 pmid=4552986		#Zamenhof-JBacteriol-1972 pmid=4552986
		+	#Thibodeau-Biotechniques-2004 pmid=15038156
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Reshma P. Shetty at 20:49, 18 October 2007

Reshma P. Shetty — Thu, 18 Oct 2007 20:49:14 GMT

←Older revision		Revision as of 20:49, 18 October 2007
Line 11:		Line 11:
	*You can measure lacZ activity using flow cytometry. See [http://www.cyto.purdue.edu/flowcyt/research/micrflow/jepras/jepras2.htm A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion]		*You can measure lacZ activity using flow cytometry. See [http://www.cyto.purdue.edu/flowcyt/research/micrflow/jepras/jepras2.htm A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion]
	**This paper uses [http://probes.invitrogen.com/media/pis/mp02904.pdf C<sub>12</sub>FDG] from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like ''Escherichia coli'' <cite>Plovins-ApplEnvironMicrobio-1994</cite>		**This paper uses [http://probes.invitrogen.com/media/pis/mp02904.pdf C<sub>12</sub>FDG] from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like ''Escherichia coli'' <cite>Plovins-ApplEnvironMicrobio-1994</cite>
-	*Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype <cite>Zamenhof-JBacteriol-1972</cite>. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.)	+	*Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype <cite>Zamenhof-JBacteriol-1972</cite>. '''(If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.)'''

	==Protocols==		==Protocols==

Reshma P. Shetty at 20:48, 18 October 2007

Reshma P. Shetty — Thu, 18 Oct 2007 20:48:59 GMT

←Older revision		Revision as of 20:48, 18 October 2007
Line 11:		Line 11:
	*You can measure lacZ activity using flow cytometry. See [http://www.cyto.purdue.edu/flowcyt/research/micrflow/jepras/jepras2.htm A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion]		*You can measure lacZ activity using flow cytometry. See [http://www.cyto.purdue.edu/flowcyt/research/micrflow/jepras/jepras2.htm A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion]
	**This paper uses [http://probes.invitrogen.com/media/pis/mp02904.pdf C<sub>12</sub>FDG] from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like ''Escherichia coli'' <cite>Plovins-ApplEnvironMicrobio-1994</cite>		**This paper uses [http://probes.invitrogen.com/media/pis/mp02904.pdf C<sub>12</sub>FDG] from Invitrogen. However, FDG might be a better substrate in gram-negative bacteria like ''Escherichia coli'' <cite>Plovins-ApplEnvironMicrobio-1994</cite>
		+	*Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype <cite>Zamenhof-JBacteriol-1972</cite>. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.)

	==Protocols==		==Protocols==
Line 24:		Line 25:
	#Miller-1972 isbn=0879691069		#Miller-1972 isbn=0879691069
	// original β-galactosidase assay by Miller		// original β-galactosidase assay by Miller
		+	#Zamenhof-JBacteriol-1972 pmid=4552986
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Reshma P. Shetty at 14:42, 15 October 2007

Reshma P. Shetty — Mon, 15 Oct 2007 14:42:34 GMT

←Older revision		Revision as of 14:42, 15 October 2007
Line 23:		Line 23:
	#Plovins-ApplEnvironMicrobio-1994 pmid=7811104		#Plovins-ApplEnvironMicrobio-1994 pmid=7811104
	#Miller-1972 isbn=0879691069		#Miller-1972 isbn=0879691069
		+	// original β-galactosidase assay by Miller
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]

Reshma P. Shetty at 14:39, 15 October 2007

Reshma P. Shetty — Mon, 15 Oct 2007 14:39:24 GMT

←Older revision		Revision as of 14:39, 15 October 2007
Line 22:		Line 22:
	#Ullmann-Bioessays-1992 pmid=1345751		#Ullmann-Bioessays-1992 pmid=1345751
	#Plovins-ApplEnvironMicrobio-1994 pmid=7811104		#Plovins-ApplEnvironMicrobio-1994 pmid=7811104
		+	#Miller-1972 isbn=0879691069
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]]