Beta-glucuronidase protocols
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==Plate Screen== | ==Plate Screen== | ||
===Materials=== | ===Materials=== | ||
| - | *Media of choice | + | *Media of choice |
| - | *X-gluc stock solution ( | + | *Agar |
| + | *X-gluc stock solution (50mg/mL) | ||
===Method=== | ===Method=== | ||
| - | + | #Prepare your liquid media and add the desired amount of agar (usually 1-2%). | |
| + | #Autoclave the media for the requisite time. | ||
| + | #Add 1.2μL of X-gluc stock solution for every mL of media (e.g. if making 1L of media add 1.2ml of stock solution) | ||
| + | #If desired, add antibiotic. | ||
| + | #Pour plates. | ||
===Notes=== | ===Notes=== | ||
Revision as of 01:38, 5 July 2011
Contents |
Introduction
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.
Plate Screen
Materials
- Media of choice
- Agar
- X-gluc stock solution (50mg/mL)
Method
- Prepare your liquid media and add the desired amount of agar (usually 1-2%).
- Autoclave the media for the requisite time.
- Add 1.2μL of X-gluc stock solution for every mL of media (e.g. if making 1L of media add 1.2ml of stock solution)
- If desired, add antibiotic.
- Pour plates.
