Beta-lactamase
From OpenWetWare
(Difference between revisions)
| Line 1: | Line 1: | ||
==Colorimetric reporter for B-lac activity== | ==Colorimetric reporter for B-lac activity== | ||
| + | |||
| + | ==Plasmid stabilization== | ||
| + | *Beta-lactamase gene ''bla'' or ''ampR'' is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin. | ||
NITROCEFIN (GLAXO RESEARCH 87/312)<br> | NITROCEFIN (GLAXO RESEARCH 87/312)<br> | ||
Revision as of 14:28, 5 August 2009
Colorimetric reporter for B-lac activity
Plasmid stabilization
- Beta-lactamase gene bla or ampR is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin.
NITROCEFIN (GLAXO RESEARCH 87/312)
Code: SR0112
For the rapid chromogenic detection of beta-lactamase activity.
Oxiod Inc. page on Nitrocefin
References
- ANDERSON ES and DATTA N. . pmid:14238093.
- Datta N and Richmond MH. . pmid:5328167.
- Evans J, Galindo E, Olarte J, and Falkow S. . pmid:4971890.
- Joseph Sambrook, David W. Russell. Molecular cloning. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 2001. isbn:0-87969-577-3.
- Gao W, Xing B, Tsien RY, and Rao J. . pmid:16220906.
See here for the full article.
