Beta-lactamase
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==Colorimetric reporter for B-lac activity== | ==Colorimetric reporter for B-lac activity== | ||
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| + | ==Plasmid stabilization== | ||
| + | *Beta-lactamase gene ''bla'' or ''ampR'' is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and [[carbenicillin]]. | ||
NITROCEFIN (GLAXO RESEARCH 87/312)<br> | NITROCEFIN (GLAXO RESEARCH 87/312)<br> | ||
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For the rapid chromogenic detection of beta-lactamase activity.<br> | For the rapid chromogenic detection of beta-lactamase activity.<br> | ||
[http://www.oxoid.com/uk/index.asp?mpage=iproductdetail&pre=SR0112&l=EN&x= Oxiod Inc. page on Nitrocefin] | [http://www.oxoid.com/uk/index.asp?mpage=iproductdetail&pre=SR0112&l=EN&x= Oxiod Inc. page on Nitrocefin] | ||
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| + | ==References== | ||
| + | <biblio> | ||
| + | #Anderson-Lancet-1965 pmid=14238093 | ||
| + | #Datta-BiochemJ-1966 pmid=5328167 | ||
| + | #Evans-JBacteriol-1968 pmid=4971890 | ||
| + | #MolecularCloning isbn=0-87969-577-3 | ||
| + | #Gao-JACS-2003 pmid=16220906 | ||
| + | </biblio> | ||
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| + | See [http://www.tsienlab.ucsd.edu/Publications/Gao%202003%20JACS%20-%20Novel%20Fluorogenic%20Substrates.pdf here] for the full article. | ||
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| + | [[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]] | ||
Current revision
Colorimetric reporter for B-lac activity
Plasmid stabilization
- Beta-lactamase gene bla or ampR is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin.
NITROCEFIN (GLAXO RESEARCH 87/312)
Code: SR0112
For the rapid chromogenic detection of beta-lactamase activity.
Oxiod Inc. page on Nitrocefin
References
- ANDERSON ES and DATTA N. . pmid:14238093.
- Datta N and Richmond MH. . pmid:5328167.
- Evans J, Galindo E, Olarte J, and Falkow S. . pmid:4971890.
- Joseph Sambrook, David W. Russell. Molecular cloning. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 2001. isbn:0-87969-577-3.
- Gao W, Xing B, Tsien RY, and Rao J. . pmid:16220906.
See here for the full article.
