Beta-lactamase: Difference between revisions
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==Colorimetric reporter for B-lac activity== | ==Colorimetric reporter for B-lac activity== | ||
==Plasmid stabilization== | |||
*Beta-lactamase gene ''bla'' or ''ampR'' is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and [[carbenicillin]]. | |||
NITROCEFIN (GLAXO RESEARCH 87/312)<br> | NITROCEFIN (GLAXO RESEARCH 87/312)<br> | ||
| Line 5: | Line 8: | ||
For the rapid chromogenic detection of beta-lactamase activity.<br> | For the rapid chromogenic detection of beta-lactamase activity.<br> | ||
[http://www.oxoid.com/uk/index.asp?mpage=iproductdetail&pre=SR0112&l=EN&x= Oxiod Inc. page on Nitrocefin] | [http://www.oxoid.com/uk/index.asp?mpage=iproductdetail&pre=SR0112&l=EN&x= Oxiod Inc. page on Nitrocefin] | ||
==References== | |||
<biblio> | |||
#Anderson-Lancet-1965 pmid=14238093 | |||
#Datta-BiochemJ-1966 pmid=5328167 | |||
#Evans-JBacteriol-1968 pmid=4971890 | |||
#MolecularCloning isbn=0-87969-577-3 | |||
#Gao-JACS-2003 pmid=16220906 | |||
</biblio> | |||
See [http://www.tsienlab.ucsd.edu/Publications/Gao%202003%20JACS%20-%20Novel%20Fluorogenic%20Substrates.pdf here] for the full article. | |||
[[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]] | [[Category:Material]] [[Category:Enzyme]] [[Category:Reporter]] | ||
Latest revision as of 11:28, 5 August 2009
Colorimetric reporter for B-lac activity
Plasmid stabilization
- Beta-lactamase gene bla or ampR is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin.
NITROCEFIN (GLAXO RESEARCH 87/312)
Code: SR0112
For the rapid chromogenic detection of beta-lactamase activity.
Oxiod Inc. page on Nitrocefin
References
- ANDERSON ES and DATTA N. RESISTANCE TO PENICILLINS AND ITS TRANSFER IN ENTEROBACTERIACEAE. Lancet. 1965 Feb 20;1(7382):407-9. DOI:10.1016/s0140-6736(65)90004-8 |
- Datta N and Richmond MH. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem J. 1966 Jan;98(1):204-9. DOI:10.1042/bj0980204 |
- Evans J, Galindo E, Olarte J, and Falkow S. Beta-lactamase of R factors. J Bacteriol. 1968 Oct;96(4):1441-2. DOI:10.1128/jb.96.4.1441-1442.1968 |
- ISBN:0-87969-577-3
- Gao W, Xing B, Tsien RY, and Rao J. Novel fluorogenic substrates for imaging beta-lactamase gene expression. J Am Chem Soc. 2003 Sep 17;125(37):11146-7. DOI:10.1021/ja036126o |
See here for the full article.
