BioBricks construction tutorial
From OpenWetWare
Contacts
Barry Canton, Jason Kelly, Will Bosworth
Aim
Describe the steps of a standard BioBricks assembly for two parts and include images of expected results (e.g., gels, seq files, etc). This will provide a walkthrough for someone new to BioBricks construction to practice with parts that are known together well.
Steps
Assumed starting point is from glycerol stocks of insert and vector.
Streak plates of insert and vector from glycerol stocks
Grow 5ml Cultures of each from a single colony
Miniprep plasmid DNA from cultures
Restriction digest insert and vector with appropriate enzymes
Extract vector and insert from gel
Ligate insert and vector
Transform ligation product into competent cells
Can use electroporation or chemical transformation
