BioBuilding: Synthetic Biology for Students: Lab 1--Protocol B: Difference between revisions
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#Mix this stock growth solution, by swirling the bottle or vortexing gently.<br> | #Mix this stock growth solution, by swirling the bottle or vortexing gently.<br> | ||
#If you will be using a spectrophotometer, set aside 2 ml of this mixture for each student group into a small sterile culture tube. This aliquot will serve as the blank for the spectrophotometer.<br> | #If you will be using a spectrophotometer, set aside 2 ml of this mixture for each student group into a small sterile culture tube. This aliquot will serve as the blank for the spectrophotometer.<br> | ||
#Move | #Move 75 ml of the broth solution to 125ml sterile erlenmeyer flask and add 2ml of bacteria from one of the overnight cultures, e.g. strain 1-1.<br> | ||
#Repeat the addition of 2ml of bacteria to | #Repeat the addition of 2ml of bacteria to 75 ml of broth in the erlenmeyer flasks for each of the overnight cultures. <br> | ||
#Cover the flasks with foil and start them gently stirring on the stir plates.<br> | #Cover the flasks with foil and start them gently stirring on the stir plates.<br> | ||
#Remove 2 ml from each sample to read the starting density of each. If you are testing all 4 samples you should now have 5 small test tubes (4 with bacterial dilutions and one blank).<br> | #Remove 2 ml from each sample to read the starting density of each. If you are testing all 4 samples you should now have 5 small test tubes (4 with bacterial dilutions and one blank).<br> |
Revision as of 23:06, 22 July 2011
Eau That Smell Lab |
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LAB 1: Eau that smell --Protocol B
Protocol BYou teacher will inform you if you need to conduct the Day 1 or Day 2 procedures.Day 1We will be receiving our bacterial strains with the plasmids already inserted. The strains may come in the form of a "stab" or "slant," a test tube with a small amount of bacteria on a slanted media, in which case you will have to streak out the bacteria onto a petri dish to continue the experiment. If the bacteria have arrived on petri dishes, you can proceed to Day 2.
A video of this procedure is here. Day 2:
A video of this procedure is here. Day 3:A video of this procedure is here.
Procedure, if no spectrophotometer is availableThe turbidity of the bacterial populations can be estimated using the McFarland Turbidity Scale. This method uses suspensions of a 1% BaCl2 in 1% H2SO4 that are visually similar to suspensions of various populations of E. coli.
Data TableIn your lab notebook, you will need to construct a data table as shown below for each of the samples.
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