BioMicroCenter:2100BioAnalyzer

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(New page: {{BioMicroCenter}} 200px The Agilent 2100 BioAnalyzer allows rigorous quantification of small samples of DNA, RNA, and Protein. In addition, the bioanaly...)
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The Agilent 2100 BioAnalyzer allows rigorous quantification of small samples of DNA, RNA, and Protein. In addition, the bioanalyzer is able to preform flow cytometry on small numbers of cells. Most samples submitted for analysis on the BioAnalyzer can be processed within 24 hours. <br>
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The Agilent 2100 BioAnalyzer is a nanofluidics device that preforms size fractionation and quantification of small samples of DNA, RNA, or Protein. In addition, the BioAnalyzer is able to preform flow cytometry on small numbers of cells. Samples can be run on the BioAnalyzer either by individual scientists or by the BioMicro Center. Most samples submitted to the BMC for analysis on the BioAnalyzer can be processed within 24 hours.
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== NUCLEIC ACID ==
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More information about the BioAnalyzer, including examples of the data that we generate in the BioMicro Center can be found on the '''[[BioMicroCenter:BioAnalyzer_INFO | BioAnalyzer Information]]''' page.
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The DNA and RNA chips preform gel electrophoresis on samples as small as 1ul with rigorous quantification.  
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===DNA===
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== BioMicro Center Services ==
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[http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/dnasolutions/pages/default.aspx  DNA] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/pages/gp1565.aspx  How it works] ''(Agilent)''
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Samples may be run on the BioAnalyzer either as a full service with sample preparation done by BioMicro Center technicians, or by individual scientists as supervised users following a one hour training session by [[BioMicroCenter:People | Manlin or Justin]].
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DNA arrays come in three size ranges:
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[[Image:BioMicro_BA_electro..jpg|right|400px]]
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*  25bp -  1,000bp
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* 100bp -  7,500bp
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* 100bp - 12,000bp
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Please make sure to specify which size range you are interested in when you drop off your samples.
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===RNA===
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=== Full Service ===
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[http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/smallrnaassay/pages/default.aspx  RNA] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/rnasolutions/pages/gp38725.aspx  Analysis Specifications] ''(Agilent)''
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Samples can be dropped off in the BioMicro Center for analysis along with the '''[[BioMicroCenter:Forms | SAMPLE SUBMISSION FORM]]'''. Completed analysis will be sent to your email address. Finished analysis includes the electropherogram (right) as well as automated quantification of peak volumes (mass/molarity). We also can send the data file to allow you to perform your own quantification (please indicate on the submission form).
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=== Supervised Service ===
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Samples can also be run by individual users on the BioAnalyzer after a user has received training by [[BioMicroCenter:People | Manlin or Justin]] (Core Labs only please). This service is being run on a trial basis and will be discontinued if we find the machine is abused. We do request that you use the chips in the BioMicro Center to help us turn over our inventory (so the kits are always in date). Once you are trained in the use of the BioAnalyzer you are welcome to use the machine off business hours (please use the sign in sheet next to the BioAnalyzer).  During regular business hours, you must check with someone in BMC to confirm that the machine is available.
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RNA arrays are segregated by the concentration of RNA used and the type of RNAs being analyzed
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'''IMPORTANT: BIOMICRO CENTER BUSINESS ALWAYS HAS PRIORITY ON THE BIOANALYZER'''
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* Standard RNA:     25-500 ng/ul total RNA
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* High Sensitivity: 0.05-5 ng/ul total RNA
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* small RNA:        0.05-2 ng/ul miRNAs
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== PROTEIN ==
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    '''Sample Type'''        '''Price (per chip)'''
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Tired of playing with Coomassie Blue? We can run your protein samples on the BioAnalyzer too -- and make pretty pictures! Even down to the level of sensitivity of silver stains - and quantify your bands to boot! All with only 5ul of sample.
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    --------------      ---------------
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    Training $50 (per session)
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    DNA         $40 
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    RNA                $40 
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    small RNA          $55 
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    Protein            $40 
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    High Sens. Ptn      $70 
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    Flow Cytometry      $40
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Rates are slightly higher then the cost of the individual chips to include the cost of the service contract, etc.
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Information from Agilent: [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/proteinkits/pages/default.aspx  Protein] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/pages/gp1566.aspx  How it works] ''(Agilent)''
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=== [[BioMicroCenter:BioAnalyzer_INFO | Available Analyses]] ===
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''' DNA '''
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* DNA 1000 Kit: 25bp -  1,000bp, 0.1-50ng/uL   
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* DNA 7500 Kit: 100bp - 7,500bp, 0.1-50ng/uL
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* DNA 12000 Kit: 100bp - 12,000bp, 0.1-50ng/ul
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* High Sensitivity DNA kit: 50bp - 7,000bp, 5-500pg/ul
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    Protein arrays    Protein Weight    Sensitivity
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''' RNA '''
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     * Protein 80        5kD- 80kD          60ng/ul
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* RNA Nano:     25-500 ng/ul total RNA
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    * Protein 230      14kD-230kD          30ng/ul
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* RNA Pico: 0.05-5 ng/ul total RNA
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    * High Sensitivity  10kD-250kD        0.3ng/ul
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* small RNA:        0.05-2 ng/ul miRNAs
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''' PROTEIN '''
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* Protein 80        5kD- 80kD          60ng/ul
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* Protein 230      14kD-230kD          30ng/ul
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* High Sensitivity  10kD-250kD        0.3ng/ul
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== FLOW CYTOMETRY ==
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''' FLOW CYTOMETRY '''
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In addition to quantification of Nucleic Acid, the BioAnalyzer is capable of preforming simple two color flow cytometry assays. In brief, six samples each 10 µl with 20,000 prestained cells are loaded onto the chip and the fluorescence intensities in two channels for about 750 single cells per sample are measured within 25 minutes.
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== Software ==
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Agilent is now providing software for the analysis of BioAnalyzer data to be used on your own PCs. Users have found it somewhat clunky, but it does give you more freedom. The software is available for download from BioMicro at '''http://bmc-apps.mit.edu/''' or from Agilent at http://www.chem.agilent.com/en-US/products/instruments/lab-on-a-chip/2100bioanalyzer/pages/formpage730.aspx
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Information from Agilent: [http://www.chem.agilent.com/en-US/products/instruments/lab-on-a-chip/2100bioanalyzer/cellsolutions/pages/default.aspx  Cells] - [http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/pages/gp4700.aspx How it works] ''(Agilent)''
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== [[BioMicroCenter:Pricing|PRICES]] ==
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    '''Sample Type'''        '''Price'''
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    --------------      ---------------
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    DNA                $10  per sample
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    RNA                $10  per sample
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    small RNA          $20  per sample
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    Protein            $10  per sample
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    High Sens. Ptn      $25  per sample
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    Flow Cytometry      $20  per sample
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== [[BioMicroCenter:Forms | FORMS]] ==
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Software Tips and Tricks
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'''Download the BioAnalyzer form [[BioMicroCenter:Forms | FORMS]]'''
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*Click the Peak Description button on the top toolbar to switch between peak height, concentration, size, molarity, and migration time.
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*Toggle the X axis to change from time to base pairs.
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*Using your own standard ladder with corresponding concentrations will generate calibrations and standard curves.
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*Customize your own color-coded flags.
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*Changing parameters on Globing Scaling will set the same scaling standards for each sample per run.

Revision as of 15:00, 6 September 2011

Image:BioMicroCenter-header6.jpg

The Agilent 2100 BioAnalyzer is a nanofluidics device that preforms size fractionation and quantification of small samples of DNA, RNA, or Protein. In addition, the BioAnalyzer is able to preform flow cytometry on small numbers of cells. Samples can be run on the BioAnalyzer either by individual scientists or by the BioMicro Center. Most samples submitted to the BMC for analysis on the BioAnalyzer can be processed within 24 hours.

More information about the BioAnalyzer, including examples of the data that we generate in the BioMicro Center can be found on the BioAnalyzer Information page.

Contents

BioMicro Center Services

Samples may be run on the BioAnalyzer either as a full service with sample preparation done by BioMicro Center technicians, or by individual scientists as supervised users following a one hour training session by Manlin or Justin.

Full Service

Samples can be dropped off in the BioMicro Center for analysis along with the SAMPLE SUBMISSION FORM. Completed analysis will be sent to your email address. Finished analysis includes the electropherogram (right) as well as automated quantification of peak volumes (mass/molarity). We also can send the data file to allow you to perform your own quantification (please indicate on the submission form).

Supervised Service

Samples can also be run by individual users on the BioAnalyzer after a user has received training by Manlin or Justin (Core Labs only please). This service is being run on a trial basis and will be discontinued if we find the machine is abused. We do request that you use the chips in the BioMicro Center to help us turn over our inventory (so the kits are always in date). Once you are trained in the use of the BioAnalyzer you are welcome to use the machine off business hours (please use the sign in sheet next to the BioAnalyzer). During regular business hours, you must check with someone in BMC to confirm that the machine is available.

IMPORTANT: BIOMICRO CENTER BUSINESS ALWAYS HAS PRIORITY ON THE BIOANALYZER

    Sample Type         Price (per chip)
    --------------      ---------------
    Training		$50 (per session)
    DNA		        $40   
    RNA                 $40  
    small RNA           $55  
    Protein             $40  
    High Sens. Ptn      $70  
    Flow Cytometry      $40 
    

Rates are slightly higher then the cost of the individual chips to include the cost of the service contract, etc.

Available Analyses

DNA

  • DNA 1000 Kit: 25bp - 1,000bp, 0.1-50ng/uL
  • DNA 7500 Kit: 100bp - 7,500bp, 0.1-50ng/uL
  • DNA 12000 Kit: 100bp - 12,000bp, 0.1-50ng/ul
  • High Sensitivity DNA kit: 50bp - 7,000bp, 5-500pg/ul

RNA

  • RNA Nano: 25-500 ng/ul total RNA
  • RNA Pico: 0.05-5 ng/ul total RNA
  • small RNA: 0.05-2 ng/ul miRNAs

PROTEIN

  • Protein 80 5kD- 80kD 60ng/ul
  • Protein 230 14kD-230kD 30ng/ul
  • High Sensitivity 10kD-250kD 0.3ng/ul

FLOW CYTOMETRY

Software

Agilent is now providing software for the analysis of BioAnalyzer data to be used on your own PCs. Users have found it somewhat clunky, but it does give you more freedom. The software is available for download from BioMicro at http://bmc-apps.mit.edu/ or from Agilent at http://www.chem.agilent.com/en-US/products/instruments/lab-on-a-chip/2100bioanalyzer/pages/formpage730.aspx

Software Tips and Tricks

  • Click the Peak Description button on the top toolbar to switch between peak height, concentration, size, molarity, and migration time.
  • Toggle the X axis to change from time to base pairs.
  • Using your own standard ladder with corresponding concentrations will generate calibrations and standard curves.
  • Customize your own color-coded flags.
  • Changing parameters on Globing Scaling will set the same scaling standards for each sample per run.
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