BioMicroCenter:2100BioAnalyzer
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
The Agilent 2100 BioAnalyzer allows rigorous quantification of small samples of DNA, RNA, and Protein. In addition, the bioanalyzer is able to preform flow cytometry on small numbers of cells. Most samples submitted for analysis on the BioAnalyzer can be processed within 24 hours.
NUCLEIC ACID
The DNA and RNA chips preform gel electrophoresis on samples as small as 1ul with rigorous quantification.
DNA
DNA - How it works (Agilent)
DNA arrays come in three size ranges:
- 25bp - 1,000bp
- 100bp - 7,500bp
- 100bp - 12,000bp
Please make sure to specify which size range you are interested in when you drop off your samples.
RNA
RNA - Analysis Specifications (Agilent)
RNA arrays are segregated by the concentration of RNA used and the type of RNAs being analyzed
- Standard RNA: 25-500 ng/ul total RNA
- High Sensitivity: 0.05-5 ng/ul total RNA
- small RNA: 0.05-2 ng/ul miRNAs
PROTEIN
Tired of playing with Coomassie Blue? We can run your protein samples on the BioAnalyzer too -- and make pretty pictures! Even down to the level of sensitivity of silver stains - and quantify your bands to boot! All with only 5ul of sample.
Information from Agilent: Protein - How it works (Agilent)
Protein arrays Protein Weight Sensitivity * Protein 80 5kD- 80kD 60ng/ul * Protein 230 14kD-230kD 30ng/ul * High Sensitivity 10kD-250kD 0.3ng/ul
FLOW CYTOMETRY
In addition to quantification of Nucleic Acid, the BioAnalyzer is capable of preforming simple two color flow cytometry assays. In brief, six samples each 10 µl with 20,000 prestained cells are loaded onto the chip and the fluorescence intensities in two channels for about 750 single cells per sample are measured within 25 minutes.
Information from Agilent: Cells - How it works (Agilent)
PRICES
Sample Type Price
-------------- ---------------
DNA $10 per sample
RNA $10 per sample
small RNA $20 per sample
Protein $10 per sample
High Sens. Ptn $25 per sample
Flow Cytometry $20 per sample
FORMS
Download the BioAnalyzer form FORMS
SOFTWARE
A downloadable version of the BioAnalyzer software is available for PCs from Agilent. You can obtain a copy of it here: http://biomicro-bioanalyzer.mit.edu/
