BioMicroCenter:ChIP - Revision history

Kevin K. Thai: /* ChIP-seq */

2012-07-05T13:45:59Z

ChIP-seq

←Older revision		Revision as of 13:45, 5 July 2012
Line 38:		Line 38:
	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.		IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
	\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>
-	\|-	+	\|}
		+	{\|
		+	\|- valign="top"
	\|rowspan="2" width=350\| '''Material Required''' <BR>		\|rowspan="2" width=350\| '''Material Required''' <BR>
	Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.		Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.

Kevin K. Thai: /* ChIP-seq */

2012-07-05T13:44:55Z

ChIP-seq

←Older revision		Revision as of 13:44, 5 July 2012
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-	{\| ~~cellpadding="10"~~	+	{\|
-	\|-	+	\|- valign="top"
	\|rowspan="2" width=350\| '''Antibody Tests''' <BR>		\|rowspan="2" width=350\| '''Antibody Tests''' <BR>
	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.		IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.

Kevin K. Thai: /* ChIP-seq */

2012-07-05T13:40:20Z

ChIP-seq

←Older revision		Revision as of 13:40, 5 July 2012
Line 38:		Line 38:
	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.		IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
	\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>
-	\|}
	\|-		\|-
	\|rowspan="2" width=350\| '''Material Required''' <BR>		\|rowspan="2" width=350\| '''Material Required''' <BR>
	Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.		Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.
	\|[[Image:ChIPmaterial.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:ChIPmaterial.jpg\|thumb\|center\|300px]] <BR>
-		+	\|}

	== ChIP Seq Literature ==		== ChIP Seq Literature ==

Kevin K. Thai at 13:39, 5 July 2012

2012-07-05T13:39:32Z

←Older revision		Revision as of 13:39, 5 July 2012
Line 38:		Line 38:
	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.		IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
	\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>
		+	\|}
	\|-		\|-
	\|rowspan="2" width=350\| '''Material Required''' <BR>		\|rowspan="2" width=350\| '''Material Required''' <BR>
	Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.		Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.
	\|[[Image:ChIPmaterial.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:ChIPmaterial.jpg\|thumb\|center\|300px]] <BR>
-	\|}	+

	== ChIP Seq Literature ==		== ChIP Seq Literature ==

Kevin K. Thai at 13:36, 5 July 2012

2012-07-05T13:36:55Z

←Older revision		Revision as of 13:36, 5 July 2012
Line 36:		Line 36:
	\|-		\|-
	\|rowspan="2" width=350\| '''Antibody Tests''' <BR>		\|rowspan="2" width=350\| '''Antibody Tests''' <BR>
-	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC. ~~<BR>~~	+	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
	\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>
		+	\|-
		+	\|rowspan="2" width=350\| '''Material Required''' <BR>
		+	Please refer to the chart on the right for materials required from users before proceeding with ChIP prep on the IP-Star. When submitting samples, please fill out a submission form stating the concentrations and catalog number of each antibody, as well as the downstream applications following ChIP. If you would like to pick up your samples for ChIP-qPCR, you can coordinate that with Shmulik.
		+	\|[[Image:ChIPmaterial.jpg\|thumb\|center\|300px]] <BR>
	\|}		\|}

Kevin K. Thai: /* Sample Preparation */

2012-07-05T13:31:17Z

Sample Preparation

←Older revision		Revision as of 13:31, 5 July 2012
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	10. Submit lysate for ChIP		10. Submit lysate for ChIP

-	Sonication conditions vary broadly between different cell types and different sonicators so you will want to optimize your conditions to produce material in the 100-~~500nt rage~~. We strongly recommend that you not freeze your lysate before submitting ~~it for~~ ChIP. Therefore, it is critical that you coordinate with BioMicro Center staff members to be sure that space is available on the IP-Star when your cells are ready. For scheduling, you may email Shmulik Matola at Shmulikm@mit.edu.	+	Sonication conditions vary broadly between different cell types and different sonicators so you will want to optimize your conditions to produce material in the 100-500bp range. We strongly recommend that you not freeze your lysate before submitting to ChIP prep. Therefore, it is critical that you coordinate with BioMicro Center staff members to be sure that space is available on the IP-Star when your cells are ready. For scheduling, you may email Shmulik Matola at Shmulikm@mit.edu.

	=== IP Conditions ===		=== IP Conditions ===

Kevin K. Thai: /* ChIP-seq */

2012-07-05T13:28:01Z

ChIP-seq

←Older revision		Revision as of 13:28, 5 July 2012
Line 36:		Line 36:
	\|-		\|-
	\|rowspan="2" width=350\| '''Antibody Tests''' <BR>		\|rowspan="2" width=350\| '''Antibody Tests''' <BR>
-	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.	+	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC. <BR>
	\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>		\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>
	\|}		\|}

Kevin K. Thai: /* ChIP-seq */

2012-07-03T15:03:31Z

ChIP-seq

←Older revision		Revision as of 15:03, 3 July 2012
Line 37:		Line 37:
	\|rowspan="2" width=350\| '''Antibody Tests''' <BR>		\|rowspan="2" width=350\| '''Antibody Tests''' <BR>
	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.		IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
		+	\|[[Image:AntibodyTests.jpg\|thumb\|center\|300px]] <BR>
	\|}		\|}

Kevin K. Thai: /* ChIP-seq */

2012-07-03T15:02:34Z

ChIP-seq

←Older revision		Revision as of 15:02, 3 July 2012
Line 36:		Line 36:
	\|-		\|-
	\|rowspan="2" width=350\| '''Antibody Tests''' <BR>		\|rowspan="2" width=350\| '''Antibody Tests''' <BR>
-	\|IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.	+	IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
	\|}		\|}

Kevin K. Thai: /* Antibody Tests */

2012-07-03T15:02:11Z

Antibody Tests

←Older revision		Revision as of 15:02, 3 July 2012
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-	=~~= Antibody Tests ==~~	+	{\| cellpadding="10"
-		+	\|-
-	\|-	+	\|rowspan="2" width=350\| '''Antibody Tests''' <BR>
	\|IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.		\|IP-Star conditions were initially validated using V6.5 ESCs. Select antibodies were used for testing, and the following chart contains some of the results. For more information about specific conditions and robustness of antibodies used, please contact Shmulik Matola at the BMC.
-	\|-	+	\|}

	== ChIP Seq Literature ==		== ChIP Seq Literature ==