BioMicroCenter:DNA Sample Preparation: Difference between revisions
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SPRIworks DNA sample preparation is performed on the [[BioMicroCenter:SPRI-Works|SPRI-te Nucleic Acid Extractor]] from Beckman Coulter Genomics. The SPRI-te automates end-repair, A-tailing, adapter ligation, and size-selection, saving hours of hands-on time. Samples are then amplified by PCR. <br> | SPRIworks DNA sample preparation is performed on the [[BioMicroCenter:SPRI-Works|SPRI-te Nucleic Acid Extractor]] from Beckman Coulter Genomics. The SPRI-te automates end-repair, A-tailing, adapter ligation, and size-selection, saving hours of hands-on time. Samples are then amplified by PCR. <br> | ||
Revision as of 14:38, 18 June 2012
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SPRIworks DNA sample preparation is performed on the SPRI-te Nucleic Acid Extractor from Beckman Coulter Genomics. The SPRI-te automates end-repair, A-tailing, adapter ligation, and size-selection, saving hours of hands-on time. Samples are then amplified by PCR.
In order to test the performance of the SPRI-te, the same ChIP-seq sample underwent preparation for Illumina sequencing by both an experienced technician and on the SPRI-te. The numbers of unique sequencing reads and significant peaks were doubled in the SPRI-te samples when compared to the manual preparations.
SPRI-te prepared samples have produced high-quality data even when sample input was below the manufacturer's recommendations. Varying quantities (200ng, 50, 12.5, 3.2, 0.8 and 0.2) of 50bp ladder from New England Biolabs were prepared on the SPRI-te and yielded similar concentrations and nearly identical traces when analyzed by BioAnalyzer.
