BioMicroCenter:Genome Seq: Difference between revisions
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== Genome Seq == | == Genome Seq == | ||
[[Image:BioMicroCenter-72_72_errorRate.png | 400px | right]] | |||
De novo sequencing can be done using the Genome Analyzer. | ''De novo'' sequencing can be done using the Genome Analyzer. Read lengths have recently been increased and data associated with these long reads look very good! http://illumina.com/downloads/ch2-ILMN_ProdGuide_DNAsequencing.pdf | ||
http://illumina.com/downloads/ch2-ILMN_ProdGuide_DNAsequencing.pdf | |||
== Sample Submission Guidelines == | == Sample Submission Guidelines == | ||
Revision as of 13:38, 31 March 2009
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Genome Seq
De novo sequencing can be done using the Genome Analyzer. Read lengths have recently been increased and data associated with these long reads look very good! http://illumina.com/downloads/ch2-ILMN_ProdGuide_DNAsequencing.pdf
Sample Submission Guidelines
Users are required to prepare their own libraries for sequencing by following the corresponding Illumina Protocols. If this is a service that you would like the BMC to perform in the future please visit the Ideas page.
Samples which are ready for sequencing should be gel-purified according to your protocol, and provided as at least 5uL of solution at a concentration of at least 10 nM in EB/ Tween 0.1%. To calculate your sample’s molar concentration, you must know the average length of your product.
When submitting a sample you must supply the BMC with the molar concentration, average length, and species of origin of all samples.
We encourage all those submitting samples to allow us to run QC analysis, using the 2100 BioAnalyzer, to obtain/confirm concentration information. Cyber green and qPCR QC analysis also coming soon!
Samples generated using standard kits with Illumina PCR primers can be submitted without sequencing primers. Custom sample preparations using customized PCR primer sequences should be provided along with a complementary sequencing primer.
Data
Images acquired from the solexa sequencer are processed through the bundled solexa inage extraction pipeline to get the sequence and quality score for each base. The data is aligned to a reference genome, if requested, using an interative ELAND algorithm. An in-depth QC report is included in the package. Sample QC report is here.
Turnaround Time
Each Genome Analyzer processes 8 samples per run, or 7 samples plus a control. The control is typically used to improve sequence quality. Full flowcells can be run usually within two weeks of submission. Partial submissions of less than eight samples (or 7 with control) are put into a project queue, where they join existing samples or await others before processing. Wait times for partial submissions vary depending on demand from other users.
Once the run has begun approximately 10 days are required for clustering, sequencing, and data analysis for a Paired End read (36 bp read x 2).
Pricing
Illumina sequencing is currently available only for labs associated with the BioMicro Center Core departments. Sequencing is currently available at an introductory price:
Paired End Reads (priced per lane):
7 lane run | 8 lane run
$1105 | $980
Genome Seq Literature
<biblio>
- Paper1 pmid=18677319
