BioMicroCenter:Multiplexed Sequencing: Difference between revisions
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(New page: {{BioMicroCenter}} == Multiplexed Sequencing == Multiplexed sequencing is a process of applying a "tag" to samples during sample preparation and thus allowing for multisample pooling w...) |
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== Multiplexed Sequencing == | == Multiplexed Sequencing == | ||
Multiplexed sequencing is a process of applying a "tag" to samples during sample preparation | Multiplexed sequencing is a process of applying a "tag" or "barcode" to samples during sample preparation, allowing up to 12 sampels to be run in the same lane. The tags are read out as a second read following repriming using the [[PEM]]. The samples are differentiated during the pipeline analysis. This application can be useful when studying samples when you need less then 3-6 million reads per sample by helping to decrease the cost and time of sequencing. | ||
The kit provided by Illumina supplies 12 oligos containing unique barcodes 6nt in length in addition to the standard primers used for sequencing. For more information visit: http://www.illumina.com/downloads/SQ_Indexing_770-2008-011.pdf | |||
Revision as of 14:04, 8 January 2009
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Multiplexed Sequencing
Multiplexed sequencing is a process of applying a "tag" or "barcode" to samples during sample preparation, allowing up to 12 sampels to be run in the same lane. The tags are read out as a second read following repriming using the PEM. The samples are differentiated during the pipeline analysis. This application can be useful when studying samples when you need less then 3-6 million reads per sample by helping to decrease the cost and time of sequencing.
The kit provided by Illumina supplies 12 oligos containing unique barcodes 6nt in length in addition to the standard primers used for sequencing. For more information visit: http://www.illumina.com/downloads/SQ_Indexing_770-2008-011.pdf
